公开(公告)号：SG10201402770YA

公开(公告)日：2014-08-28

申请号：SG10201402770Y

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： MAY ANDREW ,  CHEN PEILIN ,  WANG JUN ,  KAPER FIONA ,  ANDERSON MEGAN

Abstract: The present invention provides an elastomer microfluidic device that enables simultaneous amplification of multiple nucleic acid samples with multiple pairs of primers in a matrix format as well as recovery of the products e.g. for next generation sequencing. The flow of samples and reagents as well as the compartmentalization of individual reactions in the multiple channels and chambers is controlled using valves and dilation pumping / volumetric capacitive pumping.

公开(公告)号：CA3018687A1

公开(公告)日：2010-10-07

申请号：CA3018687

申请日：2010-04-02

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  KAPER FIONA ,  MAY ANDREW ,  WANG JUN

IPC: G01N33/48 ,  B81B3/00 ,  B81B7/04 ,  C12M1/34 ,  C12M1/36 ,  C12Q1/6853

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.