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    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    21.
    发明申请
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 审中-公开
    在甲醇存在下增强特定长度脂肪醇的微生物生产方法

    公开(公告)号：WO2015077752A1

    公开(公告)日：2015-05-28

    申请号：PCT/US2014/067282

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti ,  ANDRAE, Stefan

    IPC: C12N1/19 ,  C12N1/15 ,  C12N1/13 ,  C12N1/11

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

    Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

    Abstract translation: 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶衰减，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明还提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。

    METHODS FOR INCREASING PRODUCT YIELDS
    24.
    发明申请
    METHODS FOR INCREASING PRODUCT YIELDS 审中-公开
    增加产品线的方法

    公开(公告)号：WO2013012975A1

    公开(公告)日：2013-01-24

    申请号：PCT/US2012/047278

    申请日：2012-07-19

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P1/04 ,  C12N1/21

    CPC classification number: C12P7/04 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a precursor. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    Abstract translation: 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为前体的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE
    27.
    发明申请
    MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF FUMARATE, MALATE, AND ACRYLATE 审中-公开
    富马酸和丙烯酸的生物合成的微生物和方法

    公开(公告)号：WO2009155382A1

    公开(公告)日：2009-12-23

    申请号：PCT/US2009/047715

    申请日：2009-06-17

    Applicant: GENOMATICA, INC. ,  BURK, Mark, J. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti

    Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti

    IPC: C12P5/02 ,  C12P7/42

    CPC classification number: C12P7/46 ,  C12N9/88 ,  C12P7/40

    Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.

    Abstract translation: 非天然存在的真核生物或原核生物包括当基因破坏降低酶的活性时，编码在赋予生物体中增加的富马酸盐，苹果酸或丙烯酸酯产生的酶的基因中发生的一种或多种基因破坏。 一个或多个基因破坏使丙烯酸酯的生产增加到生物体上。 生产丙烯酸酯的生物体具有丙烯酸酯途径，至少一种编码丙烯酸酯途径酶的外源核酸以足够的量表达以产生丙烯酸酯，丙烯酸酯途径包含脱羧酶。 产生富马酸盐，苹果酸盐或丙烯酸盐的方法包括培养这些生物体。

    MICROORGANISMS FOR PRODUCING 4C-5C COMPOUNDS WITH UNSATURATION AND METHODS RELATED THERETO
    30.
    发明申请
    MICROORGANISMS FOR PRODUCING 4C-5C COMPOUNDS WITH UNSATURATION AND METHODS RELATED THERETO 审中-公开
    用于生产具有不均匀性的4C-5C化合物的微生物和与之相关的方法

    公开(公告)号：WO2016004334A1

    公开(公告)日：2016-01-07

    申请号：PCT/US2015/039037

    申请日：2015-07-02

    Applicant: GENOMATICA, INC.

    Inventor： CULLER, Stephanie J. ,  BURK, Mark J. ,  OSTERHOUT, Robin E. ,  PHARKYA, Priti

    IPC: C12N15/70 ,  C12P1/04 ,  C12P5/02 ,  C12P7/16

    CPC classification number: C12P7/04 ,  C12N15/52 ,  C12P5/026 ,  C12P7/40 ,  Y02E50/343

    Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol,. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

    Abstract translation: 本发明提供具有丁二烯，巴豆醇，2,4-戊二烯酸酯，3-丁烯-2-醇或3-丁烯-1-醇途径的非天然存在的微生物生物。 微生物生物体含有至少一种编码途径中酶的外源核酸。 本发明另外提供了生产丁二烯，巴豆醇，2,4-戊二烯酸酯，3-丁烯-2-醇或3-丁烯-1-醇的方法。 该方法可以包括培养丁二烯，巴豆醇，2,4-戊二烯酸酯，3-丁烯-2-醇或3-丁烯-1-醇生产微生物，其中微生物生物表达至少一种外源核酸编码 巴豆醇，2,4-戊二烯酸酯，3-丁烯-2-醇或3-丁烯-1-醇的条件下，足够的条件和足够的时间段的途径酶。

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