中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

84 records (took 0.071 seconds)

    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    1.
    发明申请
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 审中-公开
    在甲醇存在下增强特定长度脂肪醇的微生物生产方法

    公开(公告)号：WO2015077752A1

    公开(公告)日：2015-05-28

    申请号：PCT/US2014/067282

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti ,  ANDRAE, Stefan

    IPC: C12N1/19 ,  C12N1/15 ,  C12N1/13 ,  C12N1/11

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

    Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

    Abstract translation: 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶衰减，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明还提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。

    MICROORGANISMS AND METHODS FOR PRODUCING (3R)-HYDROXYBUTYL (3R)-HYDROXYBUTYRATE
    2.
    发明申请
    MICROORGANISMS AND METHODS FOR PRODUCING (3R)-HYDROXYBUTYL (3R)-HYDROXYBUTYRATE 审中-公开
    （3R） - 羟基丁酸（3R） - 羟基丁酸酯的微生物和方法

    公开(公告)号：WO2014190251A1

    公开(公告)日：2014-11-27

    申请号：PCT/US2014/039322

    申请日：2014-05-23

    Applicant: GENOMATICA, INC.

    Inventor： ADELSTEIN, Benjamin ,  OSTERHOUT, Robin, E. ,  BURK, Mark, J.

    IPC: C12P1/04

    CPC classification number: C12P7/62 ,  A23L33/135 ,  A23V2002/00 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07B2200/05 ,  C07C69/675 ,  C12N9/18 ,  C12N15/52 ,  C12P7/42 ,  C12R1/01 ,  C12R1/645 ,  C12Y101/01035 ,  C12Y101/011 ,  C12Y301/01067 ,  C12Y301/08001 ,  Y02P20/127

    Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-l,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)- hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)- hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.

    Abstract translation: 本文提供了具有生产（3R） - （3R） - 羟基丁酸酯途径的非天然存在的微生物生物，其中生物体还可包含（R）-1,3-丁二醇途径，（3R） - 羟基丁酸酯 途径，（3R） - 羟基丁酰辅酶A途径，乙酰乙酸途径，乙酰乙酰辅酶A途径，（3R） - 羟基丁基ACP途径或乙酰乙酰-ACP途径。 另外提供了使用微生物生物体和（3R） - 羟基丁基（3R） - 羟基丁酸酯生产和分离（3R） - （3R） - 羟基丁酸的羟基丁酸的方法和方法。 还提供了使用本发明的微生物生物体产生的（3R） - 羟基丁酸（3R） - 羟基丁酸酯来治疗或预防疾病，病症或病症的方法。

    METHODS FOR INCREASING PRODUCT YIELDS
    5.
    发明申请
    METHODS FOR INCREASING PRODUCT YIELDS 审中-公开
    增加产品线的方法

    公开(公告)号：WO2013012975A1

    公开(公告)日：2013-01-24

    申请号：PCT/US2012/047278

    申请日：2012-07-19

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P1/04 ,  C12N1/21

    CPC classification number: C12P7/04 ,  C12N15/52 ,  C12P7/18 ,  C12P7/42

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a reductive TCA or Wood-Ljungdahl pathway in which at least one exogenous nucleic acid encoding these pathway enzymes is expressed in a sufficient amount to enhance carbon flux through acetyl-CoA. A method for enhancing carbon flux through acetyl-CoA includes culturing theses non-naturally occurring microbial organisms under conditions and for a sufficient period of time to produce a product having acetyl-CoA as a precursor. Another non-naturally occurring microbial organism includes at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of carbon monoxide or hydrogen, thereby increasing the yield of redox-limited products via carbohydrate-based carbon feedstock. A method for enhancing the availability of reducing equivalents in the presence of carbon monoxide or hydrogen includes culturing this organism for a sufficient period of time to produce a product.

    Abstract translation: 非天然存在的微生物体包括具有还原性TCA或Wood-Ljungdahl途径的微生物生物体，其中至少一种编码这些途径酶的外源核酸以足够的量表达以增强通过乙酰辅酶A的碳通量。 通过乙酰辅酶A增强碳通量的方法包括在条件和足够的时间内培养这些非天然存在的微生物，以产生具有乙酰辅酶A作为前体的产物。 另一种非天然存在的微生物生物体包括至少一种编码以足够量表达的酶的外源核酸，以增强在一氧化碳或氢气存在下还原当量的可用性，从而通过碳水化合物 - 碳原料。 在一氧化碳或氢气存在下增强还原当量的可用性的方法包括在足够的时间内培养该生物体以产生产物。

    MICROORGANISM FOR PRODUCING PRIMARY ALCOHOLS AND RELATED COMPOUNDS AND METHODS RELATED THERETO
    8.
    发明申请
    MICROORGANISM FOR PRODUCING PRIMARY ALCOHOLS AND RELATED COMPOUNDS AND METHODS RELATED THERETO 审中-公开
    生产主要酒精的微生物及相关化合物及其相关方法

    公开(公告)号：WO2012177726A1

    公开(公告)日：2012-12-27

    申请号：PCT/US2012/043293

    申请日：2012-06-20

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P5/02 ,  C12N15/74 ,  C12N1/00

    CPC classification number: C12P7/04 ,  C12N15/52 ,  C12P7/24 ,  C12P7/6409 ,  C12P7/6436

    Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols and at least one exogenous nucleic acid that encodes an enzyme that increases the yields of the primary alcohol by (i) enhancing carbon fixation via the reductive TCA cycle, and/or (ii) accessing additional reducing equivalents from gaseous carbon sources and/or syngas components such as CO, C02, and/or H2. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

    Abstract translation: 本发明提供了具有至少一种外源基因插入和/或一种或多种基因破坏的非天然存在的微生物生物体，所述基因破坏产生伯醇和至少一种编码增加产量的酶的外源核酸 通过（i）通过还原TCA循环增强碳固定，和/或（ii）从气态碳源和/或合成气组分如CO，CO 2和/或H 2获得另外的还原当量。 制备长链醇的方法包括培养这些非天然存在的微生物。

    MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO
    9.
    发明申请
    MICROORGANISMS FOR PRODUCING 1,3-BUTANEDIOL AND METHODS RELATED THERETO 审中-公开
    用于生产1,3-丁二醇的微生物及其相关方法

    公开(公告)号：WO2012177619A2

    公开(公告)日：2012-12-27

    申请号：PCT/US2012/043117

    申请日：2012-06-19

    Applicant: GENOMATICA, INC. ,  BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURGARD, Anthony, P. ,  BURK, Mark, J. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12N1/21 ,  C12P7/18

    CPC classification number: C12P7/18 ,  C12N15/52

    Abstract: Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3 -BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3 -BDO pathway enzyme expressed in a sufficient amount to produce 1,3 -BDO. In some embodiments, the pathway includes reducing equivalents from CO or hydrogen. In certain embodiments, a 1,3-BDO pathway proceeds by way of central metabolites pyruvate, succinate or alpha-ketoglutarate. Also provided herein is a method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    Abstract translation: 本文提供了具有1,3-丁二醇（1,3-BOD）途径的非天然存在的微生物生物，并且包含至少一种编码1,3 -BDO途径酶的外源核酸，其以足够的量表达以产生1 ，3 -BDO。 在一些实施方案中，该途径包括从CO或氢中还原当量。 在某些实施方案中，1,3-BDO途径通过中枢代谢物丙酮酸，琥珀酸或α-酮戊二酸进行。 本文还提供了一种生产1,3-BDO的方法，包括在条件和足够的时间内培养这些微生物以产生1,3-BDO。

    MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO
    10.
    发明申请
    MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO 审中-公开
    生产甲基丙烯酸和甲基丙烯酸酯的微生物及其相关方法

    公开(公告)号：WO2012135789A2

    公开(公告)日：2012-10-04

    申请号：PCT/US2012/031733

    申请日：2012-03-30

    Applicant: GENOMATICA, INC. ,  BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    Inventor： BURK, Mark, J. ,  BURGARD, Anthony, P. ,  OSTERHOUT, Robin, E. ,  SUN, Jun ,  PHARKYA, Priti

    IPC: C12P7/40

    CPC classification number: C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12P19/32

    Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2- hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.

    Abstract translation: 本发明提供具有甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。 微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。 本发明另外提供了生产甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。 该方法可以包括培养产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物，其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸，其量足以产生甲基丙烯酸， 甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯。

Patent Agency Ranking