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    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL
    1.
    发明申请
    METHODS FOR ENHANCING MICROBIAL PRODUCTION OF SPECIFIC LENGTH FATTY ALCOHOLS IN THE PRESENCE OF METHANOL 审中-公开
    在甲醇存在下增强特定长度脂肪醇的微生物生产方法

    公开(公告)号：WO2015077752A1

    公开(公告)日：2015-05-28

    申请号：PCT/US2014/067282

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti ,  ANDRAE, Stefan

    IPC: C12N1/19 ,  C12N1/15 ,  C12N1/13 ,  C12N1/11

    CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/1022 ,  C12N9/88 ,  C12N9/90 ,  C12P7/04 ,  C12Y101/01244 ,  C12Y202/01003 ,  C12Y401/02043 ,  C12Y503/01027

    Abstract: The invention provides non-naturally occurring microbial organisms having a formaldehyde fixation pathway, a formate assimilation pathway, and/or a methanol metabolic pathway in combination with a fatty alcohol, fatty aldehyde, fatty acid or isopropanol pathway, wherein the microbial organisms selectively produce a fatty alcohol, fatty aldehyde or fatty acid of a specified length or isopropanol. The microbial organisms provided advantageously enhance the production of substrates and/or pathway intermediates for the production of chain length specific fatty alcohols, fatty aldehydes, fatty acids or isopropanol. In some aspects, the microbial organisms of the invention have select gene disruptions or enzyme attenuations that increase production of fatty alcohols, fatty aldehydes or fatty acids. The invention additionally provides methods of using the above microbial organisms to produce a fatty alcohol, a fatty aldehyde, a fatty acid or isopropanol.

    Abstract translation: 本发明提供具有甲醛固定通道，甲酸同化途径和/或与脂肪醇，脂肪醛，脂肪酸或异丙醇途径组合的甲醇代谢途径的非天然存在的微生物生物，其中所述微生物有选择地产生 脂肪醇，脂肪醛或指定长度的脂肪酸或异丙醇。 提供的微生物有利地增强了用于生产链长特异性脂肪醇，脂肪醛，脂肪酸或异丙醇的底物和/或途径中间体的生产。 在一些方面，本发明的微生物有选择性的基因破坏或酶衰减，其增加脂肪醇，脂肪醛或脂肪酸的产生。 本发明还提供了使用上述微生物生产脂肪醇，脂肪醛，脂肪酸或异丙醇的方法。

    ALCOHOL DEHYDROGENASE VARIANTS
    3.
    发明申请
    ALCOHOL DEHYDROGENASE VARIANTS 审中-公开
    酒精脱水变异

    公开(公告)号：WO2015051298A2

    公开(公告)日：2015-04-09

    申请号：PCT/US2014/059135

    申请日：2014-10-03

    Applicant: GENOMATICA, INC.

    Inventor： ANDRAE, Stefan ,  KUCHINSKAS, Michael Patrick ,  LI, Jingyi ,  NAGARAJAN, Harish ,  PHARKYA, Priti

    IPC: C12P7/16 ,  C12N1/21

    CPC classification number: C12N9/0006 ,  C12P7/18 ,  C12P7/24 ,  C12Y101/01001 ,  C12Y101/01244 ,  Y02P20/127

    Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD + -dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

    Abstract translation: 本文描述了与其未修饰的对应物相比，能够分别甲醇或乙醇转化为甲醛或乙醛的至少两倍的非天然NAD +依赖性醇脱氢酶（ADH）。 描述了编码非天然醇脱氢酶的核酸，以及包含核酸的表达构建体和包含核酸或表达构建体的工程化细胞。 还描述了表达非天然NAD +依赖性醇脱氢酶的工程细胞，任选地包括一种或多种另外的代谢途径转基因，甲醇代谢途径基因，靶产物途径基因，包括细胞的细胞培养组合物，促进生产的方法 的靶产物或其中间体，包括靶产物或中间体的组合物，以及由目标产物或中间体制成的产品。

    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS
    6.
    发明申请
    MICROORGANISMS AND METHODS FOR IMPROVING PRODUCT YIELDS ON METHANOL USING ACETYL-COA SYNTHESIS 审中-公开
    使用乙酰辅酶A合成改善甲醇产品的微生物和方法

    公开(公告)号：WO2015084633A1

    公开(公告)日：2015-06-11

    申请号：PCT/US2014/067287

    申请日：2014-11-25

    Applicant: GENOMATICA, INC.

    Inventor： OSTERHOUT, Robin, E. ,  BURGARD, Anthony, P. ,  PHARKYA, Priti ,  ANDRAE, Stefan

    IPC: C12N1/19 ,  C12N1/15 ,  C12N1/13 ,  C12N1/11

    CPC classification number: C12P5/026 ,  C12N1/14 ,  C12N1/20 ,  C12N15/70 ,  C12N15/81 ,  C12P7/02 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/10 ,  Y02E50/343

    Abstract: The invention provides non-naturally occurring microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA. In some embodiments, the microbial organisms having such pathways also include pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation. The enhanced carbon flux through acetyl-CoA, in combination with pathways for generating reducing equivalents, formaldehyde fixation and/or formate assimilation can, in some embodiments, be used for production of a bioderived compound. Accordingly, in some embodiments, the microbial organisms of the invention can include a pathway capable of producing a bioderived compound of the invention. The invention still further provides a bioderived compound produced by a microbial organism of the invention, culture medium having the bioderived compound of the invention, compositions having the bioderived compound of the invention, a biobased product comprising the bioderived compound of the invention, and a process for producing a bioderived compound of the invention.

    Abstract translation: 本发明提供了非天然存在的微生物，其含有通过乙酰辅酶A的碳通量的酶途径和/或代谢修饰。 在一些实施方案中，具有这种途径的微生物生物体还包括产生还原当量的途径，甲醛固定和/或甲酸盐同化。 在一些实施方案中，通过乙酰辅酶A增强的碳通量与产生还原当量的途径，甲醛固定和/或甲酸盐同化可用于生产生物活性化合物。 因此，在一些实施方案中，本发明的微生物体可以包括能够产生本发明生物活性化合物的途径。 本发明还提供了由本发明的微生物生物产生的生物活性化合物，具有本发明的生物活性化合物的培养基，具有本发明的生物活性化合物的组合物，包含本发明的生物活性化合物的生物基产物和一种方法 用于生产本发明的生物活性化合物。

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