MEDIUM FOR DETECTING TARGET MICROBES IN A SAMPLE

    公开(公告)号:CA2204121C

    公开(公告)日:2010-12-21

    申请号:CA2204121

    申请日:1995-10-27

    Applicant: IDEXX LAB INC

    Abstract: The invention features a medium for the detection of the presence or absence of a target microbe in a liquified environmental or biological sample comprising: a) an effective amount of a vitamin, an amino acid, an element and a salt ingredients operable to allow viability and reproduction of the tai.get microbe at a rate such that any detectable change in the sample is observable within 18 hours for a sample with less than 10 target microbes per 100 ml; and b) an effective amount of one or more nutrient-indicators provided in an amount sufficient to produce a detectable characteristic signal in the medium during growth of the target microbe; and the nutrient-indicator being operable to alter a detectable characteristic of the sample if metabolized by the target microbe so as to confirm the presence or absence of the target microbe in the sample. The invention also relates to methods for detecting the presence or absence of a target microbe in a liquified environmental or biological sample using the medium.

    25.
    发明专利
    未知

    公开(公告)号:AT245485T

    公开(公告)日:2003-08-15

    申请号:AT98956241

    申请日:1998-10-27

    Applicant: IDEXX LAB INC

    Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

    METHOD FOR QUANTIFICATION OF BIOLOGICAL MATERIAL IN A SAMPLE

    公开(公告)号:GR3037056T3

    公开(公告)日:2002-01-31

    申请号:GR20010401928

    申请日:2001-10-30

    Applicant: IDEXX LAB INC

    Abstract: Method for detection of a biological material in a sample. The method includes the steps of liquifying the sample (if necessary) and pouring the liquified sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquified sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

    27.
    发明专利
    未知

    公开(公告)号:BR9608728A

    公开(公告)日:1999-06-29

    申请号:BR9608728

    申请日:1996-05-31

    Applicant: IDEXX LAB INC

    Abstract: This invention relates to a method for detecting the existence or measuring the concentration of total viable bacteria in a test sample from a food product. A medium is provided which contains three or more different enzyme substrates each having a nutrient moiety and a detectable moiety linked together. When a substrate is hydrolysed by a bacterial enzyme to create a separate detectable moiety, it causes or produces a detectable signal. These substrates produce detectable signals when any one of a phosphatase enzyme, a glycosidase enzyme or a peptidase enzyme is present in the medium.

    Device and methods for determination of analyte in a solution

    公开(公告)号:AU1281299A

    公开(公告)日:1999-05-17

    申请号:AU1281299

    申请日:1998-10-27

    Applicant: IDEXX LAB INC

    Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

    DEVICE AND METHODS FOR DETERMINATION OF ANALYTE IN A SOLUTION

    公开(公告)号:CA2307122A1

    公开(公告)日:1999-05-06

    申请号:CA2307122

    申请日:1998-10-27

    Applicant: IDEXX LAB INC

    Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

    METHOD FOR QUANTIFICATION OF BIOLOGICAL MATERIAL IN A SAMPLE

    公开(公告)号:CA2237639C

    公开(公告)日:2011-01-11

    申请号:CA2237639

    申请日:1996-11-12

    Applicant: IDEXX LAB INC

    Abstract: Method for detection of a biological material in a sample. The method includes the steps of liquefying the sample (if necessary) and pouring the liquefied sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquefied sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

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