公开(公告)号：WO2005059595A3

公开(公告)日：2005-11-24

申请号：PCT/DE2004001969

申请日：2004-09-03

Applicant: LEICA MICROSYSTEMS

Inventor： SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G02B6/34 ,  G02B21/00 ,  G02B27/14

CPC classification number: G02B6/29395 ,  G02B6/29358 ,  G02B6/29362 ,  G02B21/0024 ,  G02B27/1086 ,  G02B27/126 ,  G02B27/145

Abstract: A device for the production of a light beam having several wavelengths, particularly an illuminating light beam for a preferably confocal scan microscope, comprising a beam combining arrangement (1) used to combine laser light beams (19) of different wavelengths, wherein said arrangement combines individual beam combining devices (2), characterized in that the beam combining devices (2) are arranged in a row or according to groups parallel to each other and are respectively configured in order to inject a laser light beam (19) having a wavelength of a defined wavelength range.

Abstract translation: 用于产生多个波长的综合光束，特别是照明光束为一个优选共焦扫描显微镜，具有Strahlvereinigeranordnung的装置（1）对于不同波长的激光光束的关联（19），所述组件包括个体光束组合器（2），其特征在于 光束组合器（2）被布置在串联或基团彼此平行，并且每个为一个激光光束（19）耦合被设计成具有一特定波长范围的波长。

公开(公告)号：WO0029876A3

公开(公告)日：2001-01-04

申请号：PCT/DE9903593

申请日：1999-11-11

Applicant: LEICA MICROSYSTEMS ,  STORZ RAFAEL

Inventor： STORZ RAFAEL

IPC: G02B21/06 ,  G02B21/00 ,  G02F1/37 ,  G02F1/39 ,  H01S3/00 ,  H01S5/00

CPC classification number: G02B21/0084 ,  G02B21/0024 ,  G02B21/0032 ,  G02B21/0056

Abstract: A method for operating a preferably confocal laser scanning microscope, whereby the laser system (1) includes at least one solid laser or a diode laser. The inventive method avoids undesirable falsification of image data and is characterized in that the scanning process or acquisition of data is synchronized with a substantially continuous intensity emission phase of said laser system (1). The laser system (1) and the data acquisition system (3) are synchronized via a control unit (2).

Abstract translation: 一种用于操作优选共焦激光扫描显微镜，其中，所述激光系统（1）包括至少一个固态激光器或二极管激光器是，为了避免与至少在很大程度上连续强度发射的相位记录，所述扫描处理的图像信息的不希望的扭曲和数据的方法 激光系统（1）被同步。 激光系统（1）和数据接收系统（3）由控制单元同步（2）。

公开(公告)号：DE502004011754D1

公开(公告)日：2010-11-18

申请号：DE502004011754

申请日：2004-04-02

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  STORZ RAFAEL ,  SEYFRIED VOLKER

IPC: G02B21/06 ,  G01T1/29 ,  G02B21/26

公开(公告)号：DE10261155B4

公开(公告)日：2010-05-06

申请号：DE10261155

申请日：2002-12-20

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL

IPC: G02B21/00

公开(公告)号：DE502004004346D1

公开(公告)日：2007-08-30

申请号：DE502004004346

申请日：2004-09-17

Applicant: LEICA MICROSYSTEMS

Inventor： SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G02B21/00 ,  G02B21/06 ,  G01J3/44 ,  G02B21/16

Abstract: Separating different emission wavelengths in a scanning microscope, is new. Separating different emission wavelengths in a scanning microscope comprises: (a) scanning a specimen with an illuminating light beam by passing the illuminating light beam over the specimen using a beam deflector; (b) selectively applying each of excitation wavelengths (1, 2, 3) to the illuminating light beam during the scanning according to a pre-definable illumination scheme; (c) detecting emission light coming from the specimen using detector(s), where the detector is read out upon each selective applying of a respective excitation wavelength to provide respective corresponding detected signals; and (d) associating the detected signals with the respective excitation wavelength using the illumination scheme. The emission light includes emission wavelengths corresponding to the excitation wavelengths. An independent claim is also included for an apparatus for separating different emission wavelengths in a scanning microscope, comprising: (a) light source(s) configured to generate an illuminating light beam; (b) a beam deflector configured to scan a specimen with the illuminating light beam by passing the illuminating light beam over the specimen; (c) a light control device configured to selectively apply each of excitation wavelengths to the illuminating light beam during a scanning according to a pre-definable illumination scheme; (d) detector(s) configured to pick up emission light coming from the specimen; and (e) a processing device configured to associate the detected signals with the respective excitation wavelengths using the illumination scheme.

公开(公告)号：DE10335466B4

公开(公告)日：2005-09-01

申请号：DE10335466

申请日：2003-08-02

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  KNEBEL WERNER

IPC: G02B21/06 ,  G02B21/00 ,  G01J3/06

Abstract: The scanning microscope (1) has an incoupling apparatus (31) with which light (33) other than the light (17) proceeding from the sample (7) is coupled into the detection beam path and conveyed to the detector (21). A closure apparatus automatically closes off the incoupling apparatus from the outside, in largely light-tight fashion, when the light guide (37) guiding the other light to the incoupling apparatus is removed. An independent claim is also included for confocal scanning microscope.

公开(公告)号：DE10361176A1

公开(公告)日：2005-07-14

申请号：DE10361176

申请日：2003-12-22

Applicant: LEICA MICROSYSTEMS

Inventor： SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G02B6/34 ,  G02B21/00 ,  G02B27/14 ,  G02B21/06 ,  G02B27/10

公开(公告)号：DE10340020A1

公开(公告)日：2005-03-31

申请号：DE10340020

申请日：2003-08-28

Applicant: LEICA MICROSYSTEMS

Inventor： BIRK HOLGER ,  SEYFRIED VOLKER ,  STORZ RAFAEL

IPC: G01J3/14 ,  G01J3/28 ,  G01J3/36 ,  G01J3/44 ,  G01N21/64 ,  G02B21/00 ,  G01J3/02

Abstract: The device has a detector array (4) disposed downstream of a prism (2). The detector array has a light-insensitive region and a light-sensitive region. The prism and the detector array are matched to each other such that a selectable wavelength component of a light beam (1) hits the light-insensitive region (6) and a remaining wavelength component of the light beam hits the light-sensitive region. An independent claim is also included for a method for suppressing excitation lines in a detection light beam of a microscope.

公开(公告)号：DE19944355B4

公开(公告)日：2004-11-18

申请号：DE19944355

申请日：1999-09-16

Applicant: LEICA MICROSYSTEMS

Inventor： HARTMANN NICOLE ,  STORZ RAFAEL

IPC: G02B5/30 ,  G02B21/00

Abstract: Optical arrangement placed in the beam path of a laser scanning microscope has a light source and a spectral selection element (2). After the element (2) is an additional optical component (8). Once the light has passed through this element the dispersion and doubly refracting properties of the detection light can be detected. Typically optical components (2, 8) are acousto-optical-modulators or acousto-optical-deflectors.

公开(公告)号：DE10231776A1

公开(公告)日：2004-01-22

申请号：DE10231776

申请日：2002-07-13

Applicant: LEICA MICROSYSTEMS

Inventor： STORZ RAFAEL ,  BIRK HOLGER

IPC: G02B21/00 ,  G02B21/06 ,  G02B21/16

Abstract: In a method for scanning microscopy a specimen, that contains at least one fluorescent dye, is illuminated with illuminating light. The detection light proceeding from scan points of the specimen is detected with a spectral detector that generates spectral data for each scan point. An amplitude value is determined from the spectral data for each fluorescent dye and transferred to a processing module.