公开(公告)号：US09709557B2

公开(公告)日：2017-07-18

申请号：US14632168

申请日：2015-02-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Yue ,  Shih-Jung Huang ,  Jolene Bradford

IPC: G01N33/52 ,  C12Q1/68 ,  C09B1/516 ,  C07H21/04 ,  G01N21/64

CPC classification number: G01N33/52 ,  C07C225/36 ,  C07C237/10 ,  C07C2603/24 ,  C07D205/04 ,  C07D213/36 ,  C07D233/61 ,  C07D249/04 ,  C07D295/13 ,  C07D295/192 ,  C07H21/04 ,  C09B1/5165 ,  C12Q1/6816 ,  C12Q1/6841 ,  G01N21/6428 ,  G01N2021/6441

Abstract: Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.

公开(公告)号：US09644099B2

公开(公告)日：2017-05-09

申请号：US15180516

申请日：2016-06-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gayle Buller ,  Jixiang Liu ,  Stephen Yue ,  Jolene Ann Bradford

IPC: C07D413/04 ,  G01N33/52 ,  C09B23/01 ,  C07D409/04 ,  C07D413/14 ,  C07D417/04 ,  G01N33/58 ,  G01N21/64

CPC classification number: C09B23/0075 ,  C07D409/04 ,  C07D413/04 ,  C07D413/14 ,  C07D417/04 ,  G01N21/6486 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6441

Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

公开(公告)号：US08822695B2

公开(公告)日：2014-09-02

申请号：US13777910

申请日：2013-02-26

Applicant: Life Technologies Corporation

Inventor： Gayle Buller ,  Jixiang Liu ,  Stephen Yue ,  Jolene Bradford

IPC: C07D409/04 ,  G01N33/52

CPC classification number: C09B23/0075 ,  C07D409/04 ,  C07D413/04 ,  C07D413/14 ,  C07D417/04 ,  G01N21/6486 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6441

Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

Abstract translation: 本发明提供了在约400nm处最佳激发并具有至少约80nm的斯托克斯位移的染料化合物。 这些染料可用于检测样品中的分析物和染料缀合物的制备。

公开(公告)号：US20140076726A1

公开(公告)日：2014-03-20

申请号：US13895207

申请日：2013-05-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Matthew BEAUDET ,  William Gregory Cox ,  Stephen Yue

IPC: C12Q1/68

CPC classification number: G01N21/64 ,  C07D417/06 ,  C09B23/02 ,  C09B23/04 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N1/30 ,  G01N2001/302 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2565/125

Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.

Abstract translation: 本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。 所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此 确定核酸的存在。 花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。

公开(公告)号：US20130224735A1

公开(公告)日：2013-08-29

申请号：US13861123

申请日：2013-04-11

Applicant: Life Technologies Corporation

Inventor： Jolene BRADFORD ,  Ching-Ying Cheung ,  Shih-Jung Huang ,  Patrick Pinson ,  Stephen Yue

IPC: G01N21/64

CPC classification number: G01N33/582 ,  C07D215/10 ,  C07D215/227 ,  C07D277/72 ,  C07D277/74 ,  C07D417/04 ,  C07D417/06 ,  C07D417/14 ,  C12Q1/6876 ,  G01N21/6428 ,  G01N21/6486 ,  G01N2021/6439 ,  G01N2201/06113 ,  Y10T436/143333

Abstract: Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.