公开(公告)号：US11175204B1

公开(公告)日：2021-11-16

申请号：US15192107

申请日：2016-06-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C07D311/00 ,  G01N1/30 ,  C07D405/12 ,  C07D405/14 ,  G01N33/50

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

公开(公告)号：US20160355682A1

公开(公告)日：2016-12-08

申请号：US15180516

申请日：2016-06-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gayle BULLER ,  Jixiang Liu ,  Stephen Yue ,  Jolene Ann Bradford

IPC: C09B23/01 ,  G01N33/52

CPC classification number: C09B23/0075 ,  C07D409/04 ,  C07D413/04 ,  C07D413/14 ,  C07D417/04 ,  G01N21/6486 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6441

Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

公开(公告)号：US09513284B2

公开(公告)日：2016-12-06

申请号：US14080559

申请日：2013-11-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard Haugland ,  Wai-Yee Leung ,  Jixiang Liu

IPC: G01N33/536 ,  C07C309/38 ,  C07D207/16 ,  C07D207/46 ,  C07D211/56 ,  C07D211/62 ,  C07D401/12 ,  C07H17/08 ,  C07H19/04 ,  C07D211/60 ,  C07K14/77 ,  C07K16/18 ,  C09B57/00

CPC classification number: G01N33/536 ,  C07C309/38 ,  C07D207/16 ,  C07D207/46 ,  C07D211/56 ,  C07D211/60 ,  C07D211/62 ,  C07D401/12 ,  C07H17/08 ,  C07H19/04 ,  C07K14/77 ,  C07K16/18 ,  C09B57/001

Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.

公开(公告)号：US09400273B1

公开(公告)日：2016-07-26

申请号：US14801000

申请日：2015-07-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jixiang Liu ,  Hee Chol Kang ,  Kyle Gee ,  Christopher Langsdorf ,  Jolene Bradford ,  Gayle Buller

IPC: C12Q1/02 ,  G01N33/50 ,  C07D405/14 ,  C07D405/12

CPC classification number: G01N33/5008 ,  C07D405/12 ,  C07D405/14 ,  G01N33/533 ,  G01N2430/00

Abstract: Described herein are compounds, methods, and kits for long-term tracking of cell proliferation, differentiation, and/or function. The compounds of the present invention are novel cell-tracking reagents, efficiently excitable with a 405-nm violet laser, that provide bright fluorescence intensity, uniform cell staining, and good retention within cells as well as low toxicity toward cells.

Abstract translation: 本文描述了用于长期跟踪细胞增殖，分化和/或功能的化合物，方法和试剂盒。 本发明的化合物是能够以405nm紫激光激发的新型细胞追踪试剂，能够提供明亮的荧光强度，均匀的细胞染色和细胞内的良好保留以及对细胞的低毒性。

公开(公告)号：US20140193926A1

公开(公告)日：2014-07-10

申请号：US14080559

申请日：2013-11-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Richard HAUGLAND ,  Wai-Yee Leung ,  Jixiang Liu

IPC: G01N33/536

CPC classification number: G01N33/536 ,  C07C309/38 ,  C07D207/16 ,  C07D207/46 ,  C07D211/56 ,  C07D211/60 ,  C07D211/62 ,  C07D401/12 ,  C07H17/08 ,  C07H19/04 ,  C07K14/77 ,  C07K16/18 ,  C09B57/001

Abstract: The invention provides a novel class of reactive fluorescent agents that are based on a pyrene sulfonic acid nucleus. The agents are readily incorporated into conjugates with other species by reacting the reactive group with a group of complementary reactivity on the other species of the conjugate. Also provided are methods of using the compounds of the invention to detect and/or quantify an analyte in a sample. In an exemplary embodiment, the invention provides multi-color assays incorporating the compounds of the invention.

Abstract translation: 本发明提供了一种基于芘磺酸核的新型反应性荧光剂。 通过使反应性基团与缀合物的其他物质上的互补反应性基团反应，将药剂容易地与其它物质结合。 还提供了使用本发明化合物检测和/或定量样品中的分析物的方法。 在一个示例性实施方案中，本发明提供掺入本发明化合物的多色测定法。

公开(公告)号：US09644099B2

公开(公告)日：2017-05-09

申请号：US15180516

申请日：2016-06-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gayle Buller ,  Jixiang Liu ,  Stephen Yue ,  Jolene Ann Bradford

IPC: C07D413/04 ,  G01N33/52 ,  C09B23/01 ,  C07D409/04 ,  C07D413/14 ,  C07D417/04 ,  G01N33/58 ,  G01N21/64

CPC classification number: C09B23/0075 ,  C07D409/04 ,  C07D413/04 ,  C07D413/14 ,  C07D417/04 ,  G01N21/6486 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6441

Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

公开(公告)号：US08822695B2

公开(公告)日：2014-09-02

申请号：US13777910

申请日：2013-02-26

Applicant: Life Technologies Corporation

Inventor： Gayle Buller ,  Jixiang Liu ,  Stephen Yue ,  Jolene Bradford

IPC: C07D409/04 ,  G01N33/52

CPC classification number: C09B23/0075 ,  C07D409/04 ,  C07D413/04 ,  C07D413/14 ,  C07D417/04 ,  G01N21/6486 ,  G01N33/52 ,  G01N33/582 ,  G01N2021/6441

Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.

Abstract translation: 本发明提供了在约400nm处最佳激发并具有至少约80nm的斯托克斯位移的染料化合物。 这些染料可用于检测样品中的分析物和染料缀合物的制备。