公开(公告)号：JPS55149212A

公开(公告)日：1980-11-20

申请号：JP5695279

申请日：1979-05-11

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO

IPC: A61K9/00 ,  A61K9/02 ,  A61K9/70 ,  A61K47/32

Abstract: PURPOSE:Titled gradually releasing preparation made by dispersing methotrexate in a specific water-insoluble but hydrophilic polymer, thus being suitably applied in the cavity of uterus because of its appropriate flexibility and elasticity and used as a remedy for villioma with depressed side-effects. CONSTITUTION:Methotrexate is dispersed in a water-insoluble and hydrophilic polymer of 20-70%, preferably 25-55% water content at 37 deg.C to form a gradually releasing preparation of methotrexate. The polymer is, e.g., preferably a crosslinked homopolymer of acrylamide or N-vinylpyrrolidone or crosslinked polyvinyl alcohol. The polymer is made by mass polymerization. The effective components are prepd. by impregnation. The preparation is readily applied to the cavity of uterus by simple procedures and the irritation is reduced and it can stand the long-term staying. The elution rate of the effective components can be controlled by the water content. The concentration of methotrexate in the preparation is preferably 1-10% based on the carrier polymer.

公开(公告)号：JPH08154699A

公开(公告)日：1996-06-18

申请号：JP29843294

申请日：1994-12-01

Applicant: TORAY INDUSTRIES

Inventor： MURAO YASUO ,  KONO HAJIME ,  OSHIHARA WATARU

IPC: C12Q1/66

Abstract: PURPOSE: To obtain the subject solution, containing a cypridinoid luciferin and an acidic solute, capable of enhancing the stability of an aqueous solution of the cypridinoid luciferin, improved in luminous efficiency and stability of measured values and useful for a high-sensitivity sensing system, etc., for an enzyme immunoassay. CONSTITUTION: A cypridinoid luciferin and an acidic salute such as formic, propionic, malic, tartaric, ethylenediaminetetraacetic or citric acid are dissolved in a 10mM phosphoric acid buffer physiological saline solution, etc., at pH

公开(公告)号：JPH0377464B2

公开(公告)日：1991-12-10

申请号：JP953882

申请日：1982-01-26

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHUNTARO ,  MURAO YASUO

IPC: A61K39/00 ,  G01N33/545 ,  G02B23/14

公开(公告)号：JPS60252265A

公开(公告)日：1985-12-12

申请号：JP10897784

申请日：1984-05-29

Applicant: TORAY INDUSTRIES

Inventor： MURAO YASUO ,  HOSAKA SHIYUNTAROU

IPC: G01N33/52 ,  G01N21/76 ,  G01N33/53 ,  G01N33/532 ,  G01N33/537

Abstract: PURPOSE:To measure a biologically active material with high sensitivity by binding avidin to a water soluble org. high-polymer compd. bound with a light emitting agent and binding further biotin therewith to bring a light emitting reagent into reaction. CONSTITUTION:Avidin in bound to the water soluble org. high-polymer compd. bound with the light emitting agent. Further, the material binding biotin to the material binding specifically with a material to be measured or the biotin bound material bound to the same material, the material with which the material to be measured binds specifically and the material to be measured are brought into reaction in the case of measuring the biologically active material in the specimen. The light emitting reagent is brought into reaction with the complex formed by such reaction and the quantity of the light emitted by the reaction of the light emitting reagent bound with the complex by the biotin-avidin bond or the excess reagent remaining in the free state without binding with the complex is measured, by which the biologically active material is measured.

公开(公告)号：JPS58128326A

公开(公告)日：1983-07-30

申请号：JP953882

申请日：1982-01-26

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO

IPC: A61K39/00 ,  G01N33/545 ,  G02B23/14

Abstract: PURPOSE:To obtain the titled fine particles useful as an immunological assay reagent, by reducing the surfaces of polymeric fine particles e.g. acrylonitrile, binding the resultant functional groups directly or to other functional groups and then to an immunologically active substance. CONSTITUTION:The surfaces of polymeric fine particles, e.g. acrylonitrile or methacrylonitrile, are reduced, and the reactivity of the resultant functional groups is used and directly or bound to other functional groups and then to an immunologically active substance, e.g. a luetic treponema antigen, hepatitis B surface antigen or rubella antigen, to give the aimed fine particles containing the immobilized immunological active substance. A crosslinking monomer having at least two carbon-carbon double bonds in the molecule in an amount of 30mol% or less based on the total monomer may be added to crosslink the polymer. Divinylbenzene, divinyltoluene, etc. may be cited as the crosslinking agent.

公开(公告)号：JPS56141559A

公开(公告)日：1981-11-05

申请号：JP4361880

申请日：1980-04-04

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO ,  KAWABATA YASUROU

IPC: C08F8/00 ,  C08F8/12 ,  C08F20/00 ,  C08F20/26 ,  G01N33/543 ,  G01N33/545

Abstract: PURPOSE:To obtain the titled reagent which does not cause nonspecific flocculation and adsorption by using fine particles of a crosslinked polymer having specific repetitive units as a particulate carrier for fixing immune activity. CONSTITUTION:Fine particles of a crosslinked polymer having the repetitive units shown by the formula (R is H, methyl) and 0.03-10mu average particle sizes is used as a particulate carrier. The above-mentioned polymer is formed by (co)polymerizing, for example, 2,3-oxypropyl acrylate and/or methacrylate (the amount of using other copolymerizable monomers is about

公开(公告)号：JPS5630405A

公开(公告)日：1981-03-27

申请号：JP10661979

申请日：1979-08-23

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHIYUNTAROU ,  MURAO YASUO

IPC: C08F8/00 ,  C08F8/12 ,  G01N33/545

Abstract: PURPOSE:To obtain the title fine particle of medical importance, by the polymerization of an addition-polimerizable monomer mixture containing glycidyl methacrylate and a crosslinkable monomer in an organic solvent, followed by the hydrolysis of the precipitated fine polymer particle. CONSTITUTION:An addition-polimerizable monomer mixture, containing 50mol% or more of glycidyl methacrylate and 0.1-30mol% of crosslinkable monomer containing at least two polymerizable C-C double bonds in the molecule, is polymerized in an organic solvent such as ethyl acetate or butyl acetate, which dissolves said monomer mixture but precipitates the polymer produced by the polymerization, and the precipitated fine polymer particle is hydrolyzed, to obtain the title fine particle. The fine particle is a hydrophilic, water-insoluble fine particle, having an equilibrium moisture content of 10-90% at 25 deg.C and an average diameter of 0.1-10mu when containing moisture, and composed of a crosslinked polymer, in which at least 50mol% of the repeating unit is composed of a 2,3-dihydroxypropyl methacrylate unit.

公开(公告)号：JPH08262021A

公开(公告)日：1996-10-11

申请号：JP708796

申请日：1996-01-19

Applicant: TORAY INDUSTRIES

Inventor： MURAO YASUO ,  KONO HAJIME ,  NAKAMURA HARUJI

IPC: G01N33/535

Abstract: PURPOSE: To obtain a pyrocypris luciferase-labeled antibody whose yield is high and whose lumininescent-activity residual rate is excellent by a method wherein the antibody is prepared by an antibody to be labeled, by an anti- pyrocypris luciferase antibody and by pyrocypris luciferase. CONSTITUTION: An antibody to be labeled is reduced it is treated with an excess amount of a cross-linking agent which comprises maleimide groups at both terminals, the cross-linking operation of identical antibodies is suppressed, and the meleimide groups are introduced. Then, an anti-pyrocypris luciferase antibody is reduced, and it is reacted with the antibody which has introduced the maleimide groups, and the complex of the antibody is prepared. At this time, monoclonal antibody which is composed of a subclass unity is especially preferable as the antibody to be labeled and the anti-pyrocypris luciferase antibody. Then, the prepared complex antibody and a pyrocypris luciferase are reacted at an equal mole, and a labeled antibody is prepared.

公开(公告)号：JPS6315553B2

公开(公告)日：1988-04-05

申请号：JP17256380

申请日：1980-12-09

Applicant: TORAY INDUSTRIES

Inventor： HOSAKA SHUNTARO ,  MURAO YASUO

IPC: C08F8/00 ,  C08F8/32 ,  C08J3/20 ,  G01N33/543 ,  G01N33/545

公开(公告)号：JPS60218052A

公开(公告)日：1985-10-31

申请号：JP7262584

申请日：1984-04-13

Applicant: TORAY INDUSTRIES

Inventor： NAKANISHI TOSHIHARU ,  MURAO YASUO ,  MIURA KUMIKO

IPC: G01N21/47 ,  G01N21/82 ,  G01N33/543

Abstract: PURPOSE:To measure objectively quantitatively determined flocculation reaction even when the amount of a reagent is small by receiving and detecting a diffraction pattern formed of fine particles in a sample cell with coherent light. CONSTITUTION:When the sample cell 4 is irradiated with the coherent laser luminous flux through an expander 2, aperture 3, etc., the laser light is diffracted by fine particles in the cell 4 and the diffraction pattern is formed on the focal plane 6 of a Fourier transform lens 5. This pattern is photodetected and measured by a photoelectric tube 7 which scans the plate 6 under the control of a scanning part 8 and processed by an amplifier and filter part 11, which supplies a diffraction intensity signal to a controller part 12. Then, the controller part 12 reconstitutes a diffraction pattern distribution and compares it with a reference pattern to measure the objectively quantitatively determined flocculation reaction even when the amount of the reagent is small.