公开(公告)号：SI2844739T1

公开(公告)日：2019-11-29

申请号：SI201331572

申请日：2013-04-30

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  NOSTRO MARIA CRISTINA ,  HOLTZINGER AUDREY ,  SARANGI FARIDA

IPC: C12N5/00 ,  A61K35/00 ,  C12P21/00

公开(公告)号：SG10201708195RA

公开(公告)日：2017-11-29

申请号：SG10201708195R

申请日：2014-04-02

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  CRAFT APRIL M

Abstract: The invention relates to a method for generating chondrocytes and/or cartilage, the method comprising:a. culturing a primitive streak-like mesoderm population with a paraxial mesoderm specifying cocktail comprising a FGF agonist and a BMP inhibitor to specify a paraxial mesoderm population expressing cell surface CD73, CD105 and/or PDGFR-beta;b. generating a chondrocyte precursor population comprising:i. culturing the paraxial mesoderm population expressing CD73, CD105 and/or PDGFR-beta at a high cell density;ii. culturing the high cell density CD73+, CD105+ and/or PDGFRbeta+ paraxial mesoderm population with a TGFbeta3 agonist in serum free media to produce a high cell density Sox9+, collagen 2+ chondrocyte precursor population; andc. culturing the high cell density Sox9+, collagen 2+ chondrocyte precursor population

公开(公告)号：AU2014247679A1

公开(公告)日：2015-11-12

申请号：AU2014247679

申请日：2014-04-02

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  CRAFT APRIL M

IPC: C12N5/077 ,  A61K35/32 ,  A61P19/00 ,  C12N5/073 ,  C12Q1/02 ,  G01N33/48 ,  G01N33/50

Abstract: A method for generating chondrocytes and/or cartilage, optionally articular like non-hypertrophic chondrocyte cells and/or cartilage like tissue and/or hypertrophic chondrocyte like cells and/or cartilage like tissue, the method comprising: a. culturing a primitive streak-like mesoderm population, optionally a CD56+, PDGFRalpha+ KDR- primitive streak-like mesoderm population, with a paraxial mesoderm specifying cocktail comprising: i. a FGF agonist; ii. a BMP inhibitor; optionally Noggin, LDN-193189, Dorsomorphin; and iii. optionally one or more of a TGFbeta inhibitor, optionally SB431524; and a Wnt inhibitor, optionally DKK1, IWP2, or XAV939; to specify a paraxial mesoderm population expressing cell surface CD73, CD105 and/or PDGFR-beta; b. generating a chondrocyte precursor population comprising: i. culturing the paraxial mesoderm population expressing CD73, CD105 and/or PDGFR-beta at a high cell density optionally in serum free or serum containing media; ii. culturing the high cell density CD73+, CD105+ and/or PDGFRbeta+ paraxial mesoderm population with a TGFbeta3 agonist in serum free media to produce a high cell density Sox9+, collagen 2+ chondrocyte precursor population; and c. either i. culturing the high cell density Sox9+, collagen 2+ chondrocyte precursor population with the TGFbeta3 agonist for an extended period of time to produce an articular like non-hypertrophic chondrocyte cells and/or cartilage like tissue; or ii. culturing the high cell density Sox9+ collagen2+ chondrocyte precursor population with a BMP4 agonist for an extended period of time to produce a hypertrophic chondrocyte like cells and/or cartilage like tissue.

公开(公告)号：CA2901377A1

公开(公告)日：2014-08-21

申请号：CA2901377

申请日：2014-02-18

Applicant: HOSPITAL FOR SICK CHILDREN ,  UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

IPC: C12N5/071 ,  A61K35/12 ,  A61K35/407 ,  A61P1/16 ,  C12N5/0735 ,  C12Q1/02

Abstract: Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates.

公开(公告)号：AU2011293052A1

公开(公告)日：2013-03-28

申请号：AU2011293052

申请日：2011-08-26

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  CRAFT APRIL M ,  DUBOIS NICOLE C

IPC: C12N5/077 ,  C12N5/0775 ,  C12Q1/04 ,  C12Q1/68 ,  G01N33/567

Abstract: The present invention relates to methods for enriching pluripotent stem cell-derived cardiomyocyte progenitor cells and cardiomyocyte cells based on SIRPA expression.

公开(公告)号：AU2014321101B2

公开(公告)日：2020-12-03

申请号：AU2014321101

申请日：2014-09-12

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  WITTY ALEC DRAKE ,  KATTMAN STEVEN JAMES

IPC: C12N5/077 ,  C12Q1/68 ,  G01N33/50

Abstract: Provided are methods and products for obtaining cardiovascular lineage cells from hPSCs. The method for obtaining a cardiomyocyte lineage or an epicardial lineage cell population from human pluripotent stem cells (hPSCs) comprises one or more of the following steps: (a) contacting BMP component primed hPSCs with a cardiovascular mesoderm programming cocktail suitable for inducing the hPSCs to differentiate to a cardiovascular mesoderm cell population under conditions suitable for the programming cocktail to penetrate the hPSCs and culturing the contacted hPSCs for a period of time to generate a KDR+ and PDGFRalpha+ cardiovascular mesoderm cell population; (b) contacting the cardiovascular mesoderm cell population with a cardiovascular progenitor specification cocktail suitable to specify a NKX2-5+ or WT1+ cardiovascular progenitor cell population under conditions suitable for the specification cocktail to penetrate the cardiovascular mesoderm cell population and culturing the contacted cardiovascular mesoderm cell population for a period of time to generate a NKX2-5+ or WT1+ cardiovascular progenitor cell population; and (d) contacting the cardiovascular progenitor cell population with a maturation cocktail under conditions suitable for the maturation cocktail to penetrate the cardiovascular progenitor cell population and culturing the contacted cardiovascular progenitor population for a period of time to produce a cardiovascular population optionally cardiomyocyte lineage cells expressing cardiac troponin T (cTnT) and/or SIRPA and/or epicardial lineage cells expressing WT1.

公开(公告)号：ES2747967T3

公开(公告)日：2020-03-12

申请号：ES13784648

申请日：2013-04-30

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  NOSTRO MARIA CRISTINA ,  HOLTZINGER AUDREY ,  SARANGI FARIDA

IPC: C12N5/071 ,  A61K35/39 ,  C12N5/02 ,  C12N5/073 ,  C12P21/02

Abstract: Un procedimiento para producir células progenitoras pancreáticas NKX6-1+ a partir de una población de células endodérmicas, comprendiendo el procedimiento poner en contacto a la población de células endodérmicas con una combinación de 1) al menos un componente de EGF seleccionado a partir de EGF, un conjugado activo de EGF y un fragmento activo de EGF; y 2) nicotinamida o una sal de la misma para inducir la diferenciación de al menos una porción de la población de células endodérmicas en células progenitoras.

公开(公告)号：HK1254968A1

公开(公告)日：2019-08-02

申请号：HK18114075

申请日：2018-11-05

Applicant: UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  NOSTRO MARIA CRISTINA ,  HOLTZINGER AUDREY ,  SARANGI FARIDA

IPC: C12N20060101 ,  C12Q20060101

公开(公告)号：SG10201803131TA

公开(公告)日：2018-06-28

申请号：SG10201803131T

申请日：2014-02-18

Applicant: UNIV HEALTH NETWORK ,  HOSPITAL FOR SICK CHILDREN

Inventor： KELLER GORDON ,  OGAWA SHINICHIRO ,  GHANEKAR ANAND ,  BEAR CHRISTINE ,  KAMATH BINITA M ,  OGAWA MINA ,  SURAPISITCHAT JAMES

Abstract: METHODS FOR GENERATING HEPATOCYTES AND CHOLANGIOCYTES FROM PLURIPOTENT STEM CELLS Methods for producing hepatocyte and/or cholangiocyte lineage cells from pluripotent stem cells, the method comprising (a) specifying the extended nodal agonist treated induced endodermal cell population to obtain a cell population comprising hepatocyte and/or cholangiocyte progenitors by contacting the extended nodal agonist treated induced endodermal cell population with specification media comprising a FGF agonist and a BMP4 agonist and/or active conjugates and/or fragments thereof; (b) inducing maturation, and optionally further lineage specification and/or expansion of the hepatocyte and/or cholangiocyte progenitors of the cell population to obtain a population comprising hepatocyte lineage cells such as hepatoblasts, hepatocytes and/or cholangiocytes, the inducing maturation step comprising generating aggregates of the cell population. Optionally, the method also comprises activating the cAMP pathway within the aggregates and forming co-aggregates . [Fig. 11]

公开(公告)号：SG10201709546YA

公开(公告)日：2017-12-28

申请号：SG10201709546Y

申请日：2012-11-21

Applicant: SUNNYBROOK RES INST ,  UNIV HEALTH NETWORK

Inventor： KELLER GORDON ,  ZUNIGA-PFLUCKER JUAN CARLOS ,  KENNEDY MARION J ,  STURGEON CHRISTOPHER MICHAEL ,  DITADI ANDREA ,  AWONG GENEVE SHEANDRA

Abstract: There is described herein a method of enriching a population of stem cells for hematopoietic progenitors. The method comprises inducing hematopoietic differentiation in a population of human embryonic stem cells or human induced pluripotent stem cells; sorting the population based on expression of CD43 and at least one of CD34, CD31 and CD144; and selecting a fraction that is at least one of CD34+CD43−, CD31+CD43− and CD144+CD43−. Also provided are populations of hematopoietic progenitors obtained by the methods described herein.