公开(公告)号：EP3222728A1

公开(公告)日：2017-09-27

申请号：EP15861198.8

申请日：2015-11-19

Applicant: Institute for Basic Science

Inventor： KIM, Jin Soo ,  KOO, Tae Young

IPC: C12N15/63 ,  C12N9/16

CPC classification number: C12N15/746 ,  C12N9/16 ,  C12N9/22 ,  C12N15/11 ,  C12N15/63 ,  C12N15/8645 ,  C12N15/867

Abstract: The present invention relates to a method for regulating gene expression, comprising introducing into a cell each of a recombinant vector which expresses a first domain comprising N-terminus of a Cas9 protein, and a recombinant vector which expresses a second domain comprising C-terminus of a Cas9 protein, a composition comprising the recombinant vectors, a kit for regulating gene expression, and a method for intracellular production of Cas9 protein. Moreover, the present invention relates to a transformed cell introduced with a viral vector which packages the first domain, and a viral vector which packages the second domain, and to a composition comprising a virus produced therefrom.

Abstract translation: 本发明涉及调节基因表达的方法，包括向细胞中导入表达包含Cas9蛋白的N端的第一结构域的重组载体和表达第二结构域的重组载体，所述第二结构域包含 Cas9蛋白质，包含该重组载体的组合物，用于调节基因表达的试剂盒以及用于细胞内产生Cas9蛋白质的方法。 此外，本发明涉及导入了包装第一结构域的病毒载体和包装第二结构域的病毒载体的转化细胞，以及包含由其产生的病毒的组合物。

公开(公告)号：EP3219810A1

公开(公告)日：2017-09-20

申请号：EP15858194.2

申请日：2015-11-13

Applicant: Institute for Basic Science

Inventor： KIM, Jin Soo ,  KIM, Dae Sik ,  BAE, Sang Su

IPC: C12Q1/68 ,  C12N9/22 ,  G06F19/22

CPC classification number: C12N9/22 ,  C12Q1/68 ,  G06F19/22 ,  Y02A50/451

Abstract: The present disclosure relates to a method for detecting off-target sites of a programmable nuclease in a genome, and specifically, to a method for detecting off-target sites through data analysis by subjecting the genome isolated in vitro to programmable nucleases to cleave the genome and then performing whole genome sequencing or deep sequencing, and to a method for selecting on-target sites of a programmable nuclease, which minimizes the off-target effect, using this method. The Digenome-seq of the present disclosure can detect the off-target sites of a programmable nuclease on the genomic scale at a high degree of reproducibility, and thus can be used in the manufacture of programmable nucleases having high target specificity and the study thereof.

Abstract translation: 本发明涉及用于检测基因组中可编程核酸酶的脱靶位点的方法，具体涉及通过数据分析检测脱靶位点的方法，所述方法通过使体外分离的基因组经受可程序化的核酸酶以切割基因组 然后进行全基因组测序或深度测序，以及使用该方法选择使可脱靶效应最小化的可编程核酸酶的在靶位点的方法。 本公开的Digenome-seq可以高度重现性地在基因组规模上检测可编程核酸酶的脱靶位点，并因此可用于制备具有高靶特异性的可编程核酸酶及其研究。

公开(公告)号：EP3194578A1

公开(公告)日：2017-07-26

申请号：EP15830693.6

申请日：2015-08-06

Applicant: College of Medicine Pochon Cha University Industry-Academic Cooperation Foundation ,  Institute for Basic Science

Inventor： KIM, Jin Soo ,  HWANG, Dong Youn

IPC: C12N5/10 ,  C12N5/071 ,  C12N15/09

CPC classification number: C12N5/0696 ,  C07K14/70539 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2501/50 ,  C12N2501/70 ,  C12N2510/00 ,  C12Q1/6881 ,  C12Q2600/156

Abstract: The present invention relates to a method for producing immune-compatible cells or a cell population which comprises a step of editing one or two alleles of one or more immune-compatible antigen genes by gene deletion or modification in an isolated cell comprising at least one of the immune-compatible antigen genes selected from HLA (human leukocyte antigen)-A, HLA-B and HLA-DR, to immune-compatible cells produced by the method, and to a cell population comprising the immune-compatible cells produced by the method

Abstract translation: 本发明涉及用于产生免疫相容性细胞或细胞群的方法，其包括通过在分离的细胞中进行基因缺失或修饰来编辑一种或多种免疫相容性抗原基因的一个或两个等位基因的步骤，所述分离的细胞包含 选自HLA（人类白细胞抗原）-A，HLA-B和HLA-DR的免疫相容性抗原基因与由该方法产生的免疫相容性细胞相结合的细胞群，以及包含由该方法产生的免疫相容性细胞的细胞群 。

公开(公告)号：EP4549592A1

公开(公告)日：2025-05-07

申请号：EP23831933.9

申请日：2023-06-29

Applicant: Seoul National University R&DB Foundation ,  Institute for Basic Science

Inventor： KIM, Vic Narry ,  SEO, Jenny ,  JUNG, Soo-Jin

IPC: C12Q1/70 ,  C12Q1/6806 ,  C12N15/10

Abstract: The present disclosure relates to a novel regulatory element for enhancing RNA stability or mRNA translation; ZCCHC2 interacting with the regulatory element; and uses thereof. Being capable of increasing the expression of a target protein, the novel regulatory element for enhancing RNA stability or mRNA translation; and ZCCHC2 interacting with regulatory element according to the present disclosure are applicable in various fields, depending on the uses of the target protein.

公开(公告)号：EP4502163A1

公开(公告)日：2025-02-05

申请号：EP23775353.8

申请日：2023-03-24

Applicant: Institute for Basic Science

Inventor： KIM, Jin-Soo ,  MOK, Young Geun ,  BAE, Su-Ji

IPC: C12N15/82

Abstract: The present invention relates to a composition for base editing of plant cell organelle DNA and, in particular, to a composition and editing method for adenine-to-guanine editing of plant cell organelle DNA.

公开(公告)号：EP4349965A1

公开(公告)日：2024-04-10

申请号：EP22816510.6

申请日：2022-06-07

Applicant: Institute for Basic Science ,  Ulsan National Institute of Science and Technology

Inventor： CHO, Yoon-Kyoung ,  SUMIT, Kumar

IPC: C12N5/071 ,  C12N11/02 ,  B01J19/00

Abstract: The present invention relates to a nano-vesicle reactor and a method for producing the same. Specifically, the present invention provides a composition for producing a nano-vesicle reactor, characterized by comprising a first vesicle containing a first receptor on its surface and a second vesicle containing a second receptor on its surface, wherein the first receptor and the second receptor are bound with each other through a ligand system so that the first vesicle and the second vesicle are fused to form the nano-vesicle reactor ; and a method for producing the above nano-vesicle reactor. The nano-vesicle reactor of the present invention can be utilized in production of energy inside a cell through cellular enzymatic reaction, a drug delivered system, and a diagnostic system.

公开(公告)号：EP4248961A1

公开(公告)日：2023-09-27

申请号：EP21894778.6

申请日：2021-02-01

Applicant: Seoul National University R & DB Foundation ,  Institute for Basic Science

Inventor： KIM, V. Narry ,  KIM, Dongwan ,  KIM, Hyunjoon

IPC: A61K31/192 ,  A61K31/196 ,  A61P31/14

Abstract: The present invention relates to an antiviral composition for Betacoronavirus comprising rhein and meclofenamic acid as active components. When the antiviral composition of the present invention is used, an antiviral effect is demonstrated whereby the viral RNA level of SARS-CoV-2 and HCoV-OC43 is suppressed, and thus it is possible to treat or prevent COVID-19 or a cold.

公开(公告)号：EP4239083A2

公开(公告)日：2023-09-06

申请号：EP21886987.3

申请日：2021-11-02

Applicant: Seoul National University R & DB Foundation ,  Institute for Basic Science

Inventor： KIM, V. Narry ,  LEE, Sungyul ,  LEE, Young-suk

IPC: C12Q1/70 ,  C12Q1/6825

Abstract: The present invention relates to an RNA interactome capturing protocol and an antiviral composition discovered using same. When used, the antiviral composition and pharmaceutical composition of the present invention can effectively inhibit viral infection and suppress viral proliferation in vivo after viral infection.

公开(公告)号：EP3904518A1

公开(公告)日：2021-11-03

申请号：EP20763447.8

申请日：2020-02-25

Applicant: Seoul National University R & DB Foundation ,  Institute for Basic Science

Inventor： KIM, V. Narry ,  KIM, Haedong ,  KIM, Jimi

IPC: C12N15/113 ,  C12N9/12

Abstract: The present invention relates to a pharmaceutical composition for preventing or treating cancer, comprising a TUT4/7 expression modulator, wherein the pharmaceutical composition of the present invention can prevent cell division and hinder cancer development by inhibiting the function of TUT4/7, and can increase the amount of miR-324-5p and suppress the function of miR-324-3p.1, and therefore can be effectively used for prevention, treatment, or diagnosis of cancer.

公开(公告)号：EP3805265A1

公开(公告)日：2021-04-14

申请号：EP19814472.7

申请日：2019-06-05

Applicant: Institute for Basic Science ,  Korea Advanced Institute Of Science And Technology

Inventor： KOH, Gou Young ,  BAE, Jeomil ,  KIM, Jaeryung

IPC: C07K16/28 ,  A61K45/06 ,  A61P35/00 ,  A61K39/00

Abstract: The present invention relates to an antibody against Tie-2 or an antigen binding fragment thereof, a nucleic acid for coding same, a vector comprising said nucleic acid, a cell transformed with said vector, a method for producing said antibody or the antigen binding fragment thereof, and a composition for preventing or treating angiogenic diseases, comprising said antibody or the antigen binding fragment thereof.