公开(公告)号：BR112013011198B1

公开(公告)日：2022-03-15

申请号：BR112013011198

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： MA CHE ,  WONG CHI-HUEY ,  TSENG YUNG-CHIEH

IPC: C12N15/09 ,  C12P21/06

Abstract: métodos para produção de partículas virais com glicosilação simplificada de proteínas de superfície. métodos para produção de partículas virais com glicosilação simplificada em proteínas estruturais ou de superfície são fornecidos. quando usadas como alvos para produção de vacinas, a natureza conservada de tais sítios gera vacinas que são menos sensíveis a mutações virais. é discutido o uso de inibidores de glicosilação para produção de vírus com perfis de glicosilação simplificada. são fornecidos uma descrição ilustrativa do vírus da influenza e métodos para a produção de partículas do vírus da influenza monoglicosiladas. são fornecidos métodos para a produção de formas monoglicosiladas do vírus da influenza a, nibrg-14 (h5nl).

公开(公告)号：IL253160A

公开(公告)日：2021-09-30

申请号：IL25316017

申请日：2017-06-25

Applicant: ACADEMIA SINICA

IPC: A61K39/00 ,  G01N33/574

Abstract: The present disclosure relates to methods and compositions which can modulate the globoseries glycosphingolipid synthesis. Particularly, the present disclosure is directed to glycoenzyme inhibitor compound and compositions and methods of use thereof that can modulate the synthesis of globoseries glycosphingolipid SSEA-3/SSEA-4/GloboH in the biosynthetic pathway; particularly, the glycoenzyme inhibitors target the alpha-4GalT; beta- 4GalNAcT-I; or beta-3GalT-V enzymes in the globoseries synthetic pathway. Additionally, the present disclosure is also directed to vaccines, antibodies, and/or immunogenic conjugate compositions targeting the SSEA-3/SSEA-4/GLOBO H associated epitopes (natural and modified) which elicit antibodies and/or binding fragment production useful for modulating the globoseries glycosphingolipid synthesis. Moreover, the present disclosure is also directed to the method of using the compositions described herein for the treatment or detection of hyperproliferative diseases and/or conditions. Furthermore, the instant disclosure also relates to cancer stem cell biomarkers for disgnostic and therapeutic uses.

公开(公告)号：ES2847249T3

公开(公告)日：2021-08-02

申请号：ES17771049

申请日：2017-03-22

Applicant: ACADEMIA SINICA

Inventor： HU CHE-MING ,  CHEN HUI-WEN ,  YAO BING-YU

IPC: A61K9/51 ,  A61K31/522 ,  A61K48/00

Abstract: Una nanopartícula polimérica para encapsular un agente bioactivo hidrofílico, comprendiendo la nanopartícula polimérica una envoltura polimérica impermeable al agua, y uno o más núcleos acuosos encerrados por la envoltura polimérica, conteniendo cada uno de los uno o más núcleos acuosos el agente bioactivo hidrofílico, en donde la envoltura polimérica está formada de un polímero que contiene un segmento no polar y un grupo terminal polar y tiene un grosor de 8-20 nm y la nanopartícula polimérica tiene un diámetro externo de 100-600 nm.

公开(公告)号：ES2831256T3

公开(公告)日：2021-06-08

申请号：ES11838917

申请日：2011-11-04

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  TSENG YUNG-CHIEH

IPC: C12P21/06 ,  A61K39/145 ,  C12N7/00 ,  C12N15/09 ,  C12P21/00

Abstract: Un método para producir un virus de la gripe desglicosilado que comprende un antígeno hemaglutinina (HA) y que tiene un solo resto de GlcNAc unido a un sitio de N-glicosilación, comprendiendo el método: a) cultivar un virus de la gripe que comprende un antígeno hemaglutinina (HA) en un hospedante adecuado en presencia de una cantidad eficaz de un inhibidor de a-manosidasa I, para producir glicoproteínas Man9(GlcNAc)2, en el que el hospedante adecuado es un huevo de gallina embrionado libre de patógenos específicos (SPF); b) recuperar el virus de la gripe producido en a); c) poner en contacto el virus de la gripe recuperado en b) con una endoglicosidasa (Endo H) para producir dicho virus de la gripe desglicosilado; y d) aislar el virus de la gripe desglicosilado.

公开(公告)号：IL253161A

公开(公告)日：2021-02-28

申请号：IL25316117

申请日：2017-06-25

Applicant: ACADEMIA SINICA

IPC: A61K39/00 ,  C07H15/04 ,  C07H15/26 ,  C07K16/30 ,  C07K16/44

Abstract: The present disclosure is directed to vaccines, antibodies, and/or immunogenic conjugate compositions targeting the SSEA3/SSEA4/GloboH associated epitopes (natural and modified) which elicit antibodies and/or binding fragment production useful for modulating the globo-series glycosphingolipid synthesis. The present disclosure relates to methods and compositions which can modulate the globo-series glycosphingolipid synthesis. Particularly, the present disclosure is directed to glycoenzyme inhibitor compound and compositions and methods of use thereof that can modulate the synthesis of globo-series glycosphingolipid SSEA3/SSEA4/GloboH in the biosynthetic pathway; particularly, the glycoenzyme inhibitors target the alpha-4GalT; beta-4GalNAcT-I; or beta-3GalT-V enzymes in the globo-series synthetic pathway. Moreover, the present disclosure is also directed to the method of using the compositions described herein for the treatment or detection of hyperproliferative diseases and/or conditions.

公开(公告)号：IL250957A

公开(公告)日：2021-01-31

申请号：IL25095717

申请日：2017-03-06

Applicant: ACADEMIA SINICA

IPC: A61K39/39 ,  A61K39/00 ,  C07H15/04 ,  C07H15/18

Abstract: Glycosphingolipids (GSLs) compositions and methods for iNKT-independent induction of chemokines are disclosed.

公开(公告)号：ES2784189T3

公开(公告)日：2020-09-23

申请号：ES10756977

申请日：2010-03-26

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  MA CHE ,  WANG CHENG-CHI ,  CHEN JUINE-RUEY

IPC: A61K39/12 ,  A61K39/145

Abstract: Una composición inmunogénica que comprende: una glicoproteína hemaglutinina (HA) del virus de la gripe parcialmente glicosilada, en donde la glicoproteína HA comprende uno o más sitios de glicosilación a los que está unido un glicano, y en donde el glicano consiste en un residuo azúcar, dos residuos azúcar o tres residuos azúcar.

公开(公告)号：HK1254775A1

公开(公告)日：2019-07-26

申请号：HK18113821

申请日：2018-10-30

Applicant: ACADEMIA SINICA

Inventor： WONG CHI-HUEY ,  WU CHUNG-YI ,  TSAI TSUNG-I

IPC: C12P20060101

公开(公告)号：AU2017315677A1

公开(公告)日：2019-04-11

申请号：AU2017315677

申请日：2017-08-23

Applicant: ACADEMIA SINICA ,  CHO PHARMA INC ,  LIN NAN HORNG

Inventor： LIN NAN-HORNG ,  HUANG LIN-YA ,  SHIVATARE SACHIN S ,  CHEN LI-TZU ,  WONG CHI-HUEY ,  WU CHUNG-YI ,  CHENG TING

IPC: A61K38/47 ,  C12N9/24 ,  C12P21/02

Abstract: A mutant of EndoS2 includes one or more mutations in the sequence of a wild-type EndoS2 (SEQ ID NO: 1), wherein the one or more mutations are in a peptide region located within residues 133-143, residues 177-182, residues 184-189, residues 221-231, and/or residues 227-237, wherein the mutant of EndoS2 has a low hydrolyzing activity and a high tranglycosylation activity, as compared to those of the wild-type EndoS2. A method for preparing an engineered glycoprotein using the mutant of EndoS2 includes coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is a glycan oxazoline.

公开(公告)号：IL244326A

公开(公告)日：2019-03-31

申请号：IL24432616

申请日：2016-02-28

Applicant: ACADEMIA SINICA

IPC: A61K31/7012 ,  A61K31/739 ,  A61K35/14

Abstract: Glycosphingolipids (GSLs) bearing α-glucose (α-Glc) that preferentially stimulate human invariant NKT (iNKT) cells are provided. GSLs with α-glucose (α-Glc) that exhibit stronger induction in humans (but weaker in mice) of cytokines and chemokines and expansion and/or activation of immune cells than those with α-galactose (α-Gal) are disclosed. GSLs bearing α-glucose (α-Glc) and derivatives of α-Glc with F at the 4 and/or 6 positions are provided. Methods for iNKT-independent induction of chemokines by the GSL with α-Glc and derivatives thereof are disclosed. Methods for immune stimulation in humans using GSLs with α-Glc and derivatives thereof are provided.