公开(公告)号：US20160027629A1

公开(公告)日：2016-01-28

申请号：US14875527

申请日：2015-10-05

Applicant: Fluidigm Canada Inc.

Inventor： Alexei Antonov ,  Dmitry Roman Bandura

IPC: H01J49/00

CPC classification number: H01J49/0036 ,  H01J49/0027

Abstract: A mass spectrometer has a particle introduction system and a vaporizer, atomizer, and ionizer configured to produce ions from elements associated with the particle. An ion mass-to-charge ratio analyzer is configured to separate ions according to their mass-to-charge ratio. A detector is positioned to detect at least some of the separated ions. A digital processor is configured to: (a) acquire data from the detector including at least first data in a primary detection group defined to comprise one or more mass-to-charge ratio channels of the mass spectrometer; (b) determine whether or not ions detected during at least one sampling cycle meet at least one selection criterion indicating a presence of a particle in the mass spectrometer; and (c) determine whether or not to use data in a secondary detection group based on whether or not the at least one selection criterion is met.

Abstract translation: 质谱仪具有粒子引入系统和配置成从与颗粒相关的元件产生离子的蒸发器，雾化器和离子发生器。 离子质荷比分析仪被配置为根据其质荷比分离离子。 定位检测器以检测至少一些分离的离子。 数字处理器被配置为：（a）从检测器采集数据，其中包括被定义为包括质谱仪的一个或多个质荷比通道的初级检测组中的至少第一数据; （b）确定在至少一个采样周期期间检测到的离子是否满足指示质谱仪中颗粒存在的至少一个选择标准; 以及（c）基于是否满足所述至少一个选择标准来确定是否在次级检测组中使用数据。

公开(公告)号：US20160025731A1

公开(公告)日：2016-01-28

申请号：US14875553

申请日：2015-10-05

Applicant: FLUIDIGM CANADA INC.

Inventor： MITCHELL A. WINNIK ,  MARK NITZ ,  VLADIMIR BARANOV ,  XUDONG LOU

IPC: G01N33/574 ,  C08F220/56

CPC classification number: C08F8/32 ,  C08F8/14 ,  C08F8/42 ,  C08F220/36 ,  C08F220/56 ,  G01N33/532 ,  G01N33/57426 ,  G01N33/58 ,  Y10T436/24

Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).

公开(公告)号：US09177767B2

公开(公告)日：2015-11-03

申请号：US14309591

申请日：2014-06-19

Applicant: Fluidigm Canada Inc.

Inventor： Alexei Antonov ,  Dmitry Roman Bandura

IPC: H01J49/26 ,  H01J49/00

CPC classification number: H01J49/0036 ,  H01J49/0027

Abstract: A mass spectrometer has a particle introduction system and a vaporizer, atomizer, and ionizer configured to produce ions from elements associated with the particle. An ion mass-to-charge ratio analyzer is configured to separate ions according to their mass-to-charge ratio. A detector is positioned to detect at least some of the separated ions. A digital processor is configured to: (a) acquire data from the detector including at least first data in a primary detection group defined to comprise one or more mass-to-charge ratio channels of the mass spectrometer; (b) determine whether or not ions detected during at least one sampling cycle meet at least one selection criterion indicating a presence of a particle in the mass spectrometer; and (c) determine whether or not to use data in a secondary detection group based on whether or not the at least one selection criterion is met.

Abstract translation: 质谱仪具有粒子引入系统和配置成从与颗粒相关的元件产生离子的蒸发器，雾化器和离子发生器。 离子质荷比分析仪被配置为根据其质荷比分离离子。 定位检测器以检测至少一些分离的离子。 数字处理器被配置为：（a）从检测器采集数据，其中包括被定义为包括质谱仪的一个或多个质荷比通道的初级检测组中的至少第一数据; （b）确定在至少一个采样周期期间检测到的离子是否满足指示质谱仪中颗粒存在的至少一个选择标准; 以及（c）基于是否满足所述至少一个选择标准来确定是否在次级检测组中使用数据。

公开(公告)号：US20150011422A1

公开(公告)日：2015-01-08

申请号：US14327960

申请日：2014-07-10

Applicant: Fluidigm Canada Inc.

Inventor： Olga Ornatsky

IPC: C12Q1/42

CPC classification number: G01N33/6848 ,  C12Q1/42 ,  C12Q1/485 ,  G01N33/569 ,  G01N33/58 ,  G01N33/6842 ,  G01N2333/91205 ,  G01N2333/916 ,  G01N2440/14 ,  G01N2458/15 ,  G01N2560/00 ,  Y10S435/975

Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes

Abstract translation: 提供了涉及翻译后修饰的酶的方法和试剂盒。 该方法采用元素分析法，包括ICP-MS。 这些方法允许通过参与翻译后修饰的酶（包括激酶和磷酸酶enyzmes）方便和准确地分析底物的翻译后修饰

公开(公告)号：US12013391B2

公开(公告)日：2024-06-18

申请号：US16383241

申请日：2019-04-12

Applicant: Fluidigm Canada Inc.

Inventor： Vladimir Baranov ,  Alexander Loboda ,  Michael Sullivan

IPC: G01N33/53 ,  G01N33/58 ,  H01J49/10

CPC classification number: G01N33/5306 ,  G01N33/58 ,  H01J49/105 ,  G01N2458/15 ,  G01N2560/00

Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.

公开(公告)号：US12000838B2

公开(公告)日：2024-06-04

申请号：US17050716

申请日：2019-04-26

Applicant: Fluidigm Canada Inc.

Inventor： Alexander V. Loboda ,  Olga Ornatsky ,  Vladimir Baranov ,  Nikita Zabinyakov ,  Anastasia Mavropoulos ,  Daniel Majonis ,  Bedilu Allo ,  Taunia Closson

IPC: G01N33/68 ,  C07K1/13 ,  G01N1/28

CPC classification number: G01N33/6848 ,  C07K1/13 ,  G01N1/28 ,  G01N2458/15 ,  G01N2560/00

Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.

公开(公告)号：US20220310373A1

公开(公告)日：2022-09-29

申请号：US17620049

申请日：2020-06-17

Applicant: Fluidigm Canada Inc.

Inventor： Paul Corkum ,  Alexander Loboda ,  David M. Rayner

IPC: H01J49/00 ,  H01J49/16 ,  H01J49/04 ,  G01N15/14

Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.

公开(公告)号：US20220288246A1

公开(公告)日：2022-09-15

申请号：US17632438

申请日：2020-08-07

Applicant: Fluidigm Canada Inc. ,  THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

Inventor： Peng Liu ,  Daniel Majonis ,  Vladimir Baranov ,  Mitchel A. Winnik ,  Hyungjun Cho

IPC: A61K51/10 ,  A61K51/06

Abstract: A new class of mass-tag polymers is provided, which include enriched metal isotopes such as zirconium and hafnium mass tags. The chemistry of these new mass tags are different from that of lanthanide mass tags, and opens up new mass channels that can be used in mass cytometry. These polymers may be used for mass cytometry, therapeutic delivery of a radioactive isotope, or screening of a therapeutic isotope. Aspects include a kit, method of making, and method of using a polymer, isotopic composition, or both. A kit may include a polymer. The polymer may include pendant groups that chelate an enriched isotope, such as zirconium and/or hafnium. The kit may include an isotopic composition comprising an enriched zirconium or hafnium isotope. Polymers may be conjugated to a biologically active material. Aspects may also include making a kit. Aspects include use of a kit, such as for mass cytometry.

公开(公告)号：US20220093382A1

公开(公告)日：2022-03-24

申请号：US17470769

申请日：2021-09-09

Applicant: Fluidigm Canada Inc.

Inventor： Alexander Loboda ,  Raymond Jong ,  Michael Sullivan ,  Serguei Vorobiev ,  Robert Rotenberg ,  Emil D. Stratulativ ,  Maxim Voronov ,  Mark Armstrong

IPC: H01J49/10 ,  H01J49/00 ,  H01J49/04 ,  H05H1/24 ,  H05H1/30

Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.

公开(公告)号：US20210404968A1

公开(公告)日：2021-12-30

申请号：US17287923

申请日：2019-10-28

Applicant: Fluidigm Canada Inc.

Inventor： Alexander V. Loboda ,  Adam Carew

IPC: G01N21/73 ,  H01J49/10 ,  H05H1/30

Abstract: We describe in this application the analysis of samples using elemental or mass spectrometry and the analysis of samples, such as biological samples by suspension mass cytometry or imaging mass cytometry and an inductively coupled plasma torch with reverse vortex flow for elemental analysis and a method of operating an ICP torch configured to interface with a spectrometer.