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公开(公告)号：KR100701672B1
公开(公告)日：2007-04-03
申请号：KR1020040103965
申请日：2004-12-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: C07K14/005 , A61K39/12 , A61K2039/523 , A61K2039/54 , A61K2039/552 , C12N2710/00022 , C12N2710/00034
Abstract: 본 발명은 한국형 돌돔 이리도바이러스의 면역원성을 갖는 단백질을 암호하는 유전자, 상기 유전자에 의해 암호되는 단백질, 상기 단백질에 의해 유래되는 합성 펩타이드, 상기 유전자를 포함하는 재조합 발현 벡터, 상기 재조합 벡터를 포함하는 형질전환체, 상기 형질전환체를 불활성화시켜 제조한 불활성화 백신 및 상기 백신을 이용하여 어류를 면역시키는 방법에 관한 것이다.
돌돔 이리도바이러스, 불활성화 백신-
公开(公告)号：KR102254461B1
公开(公告)日：2021-05-21
申请号：KR1020190152303
申请日：2019-11-25
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/6888
Abstract: 본발명은종래어류의미아미엔시스아비두스 (Miamiensis avidus)에의한스쿠티카병의원인이되는기생충의유전형을판별하기위하여시퀀싱방법을이용하던불편함을해소하고자고안된것으로, 미아미엔시스아비두스미토콘드리아의일부핵산서열을특이적으로증폭함으로써 1회의 PCR 반응으로미아미엔시스아비두스유전형 I과 II를정확하고신속하게판별할수 있는장점이있다.
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Patent Agency Ranking
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公开(公告)号：KR101918140B1
公开(公告)日：2018-11-16
申请号：KR1020180068300
申请日：2018-06-14
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/689
Abstract: 본발명은퀴놀론계항생제내성균판별용 PNA 프로브및 이를이용한퀴놀론계항생제내성균판별방법에관한것으로, 더욱상세하게는비브리오균, 연쇄구균또는에드워드균의퀴놀론계항생제내성유전자마커를선정하여증폭시킨다음, 증폭산물을특이적으로인식하는 PNA를혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기융해곡선의분석을통해융해온도로부터퀴놀론계항생제내성균을판별하는방법에관한것이다. 본발명에따르면비브리오균, 연쇄구균또는에드워드균의퀴놀론계항생제내성유무를간단·신속·정확하게판별할수 있으므로, 비브리오균, 연쇄구균또는에드워드균이유발하는수산생물전염병인비브리오병, 연쇄구균병또는에드워드병으로인해생기는피해를축소시키는데유용하다.
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38.发明授权연쇄구균 감염증 원인세균인 스트렙토코카스 파라우베리스의 판별 및 검출용 유전자 마커, 및 이를 이용한 원인세균의 판별 및 검출 방법 有权用于歧视和检测链球菌的基因标记及使用该方法鉴别和检测Parauberis的方法
公开(公告)号：KR101677951B1
公开(公告)日：2016-11-21
申请号：KR1020160046421
申请日：2016-04-15
Applicant: 대한민국(관리부서:국립수산과학원)
IPC: C12Q1/68
Abstract: 본발명은어류연쇄구균감염증원인세균의판별및 검출용유전자마커, 및이를이용한원인세균의판별및 검출방법에관한것으로, 더욱자세하게는어류연쇄구균감염증원인세균인스트렙토코카스파라우베리스()의 16S rDNA 유전자를코딩하는 DNA 염기서열중 세균특이적단일염기다형성(Single nucleotide polymorphism, SNP)을포함하는유전자마커를선정하여증폭시킨다음, 증폭산물을특이적으로인식하는펩티드핵산(Peptide Nucleic Acid, PNA)을혼성화시키고, 혼성화된산물의온도조절을통해온도별융해곡선을얻고, 상기얻은융해곡선의분석을통해융해온도로부터세균종을판별하거나어류의상기세균종의감염여부를검출하는방법에관한것이다. 본발명은어류질병원인세균인스트렙토코카스파라우베리스()의판별및/또는검출용유전자마커를선정하고, 상기유전자마커에특이적인펩티드핵산및 프라이머쌍을이용하여세균종마다서로다른형광의증폭및 융해곡선을나타내게함으로써어류질병원인세균을간단ㆍ신속ㆍ정확하게판별하고, 어류의상기세균의감염여부를검출할수 있는효과가있다.
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公开(公告)号：KR1020110136515A
公开(公告)日：2011-12-21
申请号：KR1020100056588
申请日：2010-06-15
Applicant: 대한민국(관리부서:국립수산과학원)
Abstract: PURPOSE: An inactivated vaccine for preventing vibrio harveyi infection, containing vibrio harveyi HN strain as an antigen is provided to reduce mortality of fishes and to enhance productivity of fishes. CONSTITUTION: A Vibrio harveyi HN(deposit number KCTC 11705BP) strain is isolated from flatfishes with vibrio harveyi infection. An inactivated vaccine for preventing vibrio herveyi infection contains the Vibrio harveyi HN(deposit number KCTC 11705BP) strain as an antigen. The vaccine further contains inactivated Streptococcus parauberis strain. A method for manufacturing the vaccine comprises a step of culturing the Vibrio harveyi HN(deposit number KCTC 11705BP) strain and inactivating. The medium for culturing the strain contains 50-200 umol of 2,2'-dipyridyl.
Abstract translation: 目的：提供一种用于预防弧菌弧菌感染的灭活疫苗，其包含哈氏弧菌HN菌株作为抗原，以减少鱼类的死亡率并提高鱼类的生产力。 构成：哈氏弧菌HN（保藏号KCTC 11705BP）菌株与哈氏弧菌感染的扁平鱼类分离。 用于预防弧菌感染感染的灭活疫苗含有作为抗原的Vibrio harveyi HN（保藏号KCTC 11705BP）菌株。 该疫苗还含有失活的欧洲白au链球菌菌株。 制备疫苗的方法包括培养哈氏弧菌HN（保藏号KCTC 11705BP）菌株并灭活的步骤。 用于培养菌株的培养基含有50-200μmol的2,2'-联吡啶。
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公开(公告)号：KR1020070088433A
公开(公告)日：2007-08-29
申请号：KR1020070080555
申请日：2007-08-10
Applicant: 대한민국(관리부서:국립수산과학원)
CPC classification number: A61K39/092 , A61K39/40 , Y10S424/827
Abstract: A method for preparing a mixture vaccine is provided to obtain the vaccine which is able to prevent Edwardsiellosis and beta-hemolysis streptococcal disease at the same time by one shot injection so that the health fish can be maintained. The method comprises the steps of: (a) after adding 20% glycerin to Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208, preserving it at a temperature of -85 deg.C; (b) after inoculating the preserved Edwardsiella tarda FP2018 and Streptococcus iniae JSL 0208 in a BHI(brain heart infusion) solid culture medium in which 1% of NaCl is added, culturing it at a temperature of 25 deg.C for 18-24 hours; (c) after respectively suspending cultured strains in sterile saline solution to prepare a suspended solution having the absorbance of 0.5, inoculating 5 ml of each of the obtained solution to 1 liter of a BHI liquid culture medium in which 1% of NaCl is added; (d) culturing each of the strains-inoculated each Erlenmeyer flask with mixing it in a shaking incubator at a temperature of 25 deg.C with the speed of 150 rotation per minute for 24 hours; (e) after respectively putting 37% formaldehyde in each of the strain culture solution to have the formaldehyde concentration of 0.4% and leaving it at room temperature for 1 hour, leaving it at 4 deg.C for 24 hours to inactivate each of the strains; (f) after centrifuging each of the inactivated strain culture solution with the speed of 6,000 rotation per minute for 20 minutes and measuring wet weight thereof, diluting it in sterile saline solution in which 0.02% of sodium azide is added to have the wet weight of 100mg/ml and storing it at a temperature of 4 deg.C; and (g) mixing a vaccine for Edwardsiellosis and a vaccine for beta-hemolysis Streptococcal disease in a ratio of 1:1.
Abstract translation: 提供一种制备混合疫苗的方法，以获得能够通过一次注射同时预防爱德华氏病和β-溶血性链球菌疾病的疫苗，从而保持健康的鱼类。 该方法包括以下步骤：（a）在Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208中加入20％甘油后，在-85℃的温度下保存; （b）在保存的Edwardsiella tarda FP2018和Streptococcus iniae JSL 0208的BHI（脑心浸液）固体培养基中接种1％的NaCl后，在25℃的温度下培养18-24小时 ; （c）分别将培养菌株悬浮于无菌盐水溶液中以制备吸光度为0.5的悬浮液，将所得溶液5ml接种于1升加入1％NaCl的BHI液体培养基中; （d）将培养各菌株接种的每个锥形瓶，在振荡培养箱中在25℃的温度下以150转/分钟的速度混合24小时; （e）分别在每个菌株培养液中分别加入37％的甲醛使甲醛浓度为0.4％，并在室温下放置1小时，在4℃下放置24小时，使每个菌株失活 ; （f）以每分钟6,000转/分钟的速度离心每个灭活的菌株培养液20分钟并测量其湿重，将其稀释在无水盐水溶液中，其中加入0.02重量％的叠氮化钠以使湿重 100mg / ml，储存温度为4℃; 和（g）以1：1的比例混合用于爱德华氏病的疫苗和用于β-溶血性链球菌疾病的疫苗。
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