公开(公告)号：KR1020110061432A

公开(公告)日：2011-06-09

申请号：KR1020090118079

申请日：2009-12-01

Applicant: 한국과학기술연구원

Inventor： 김세훈 ,  권익찬 ,  김광명 ,  최귀원 ,  안형준 ,  임창근

IPC: B82B1/00 ,  B82B3/00 ,  C09K11/06

CPC classification number: A61K49/0017 ,  A61K49/0021 ,  A61K49/0054 ,  A61K49/1824 ,  G01N33/49 ,  G01N33/58

Abstract: PURPOSE: Aqueous-dispersed chemical-luminescent nanoparticles, a producing method thereof, and a use thereof are provided to accurately and extensively diagnose active oxygen related disease by directly imaging hydrogen peroxide. CONSTITUTION: Aqueous-dispersed chemical-luminescent nanoparticles contain the following: 0.01~3wt% of core containing a hydrophobic chemical-luminescent substance; 0.5~30wt% of surfactant surrounding the core; and 67~99.49wt% water. The luminescent wavelength of the hydrophobic chemical-luminescent substance is 650~900nm. The hydrophobic chemical-luminescent substance is formed with a peroxyoxalate derivative and a fluorescent material. The surfactant is poloxamer.

Abstract translation: 目的：通过直接成像过氧化氢，提供水分散化学发光纳米颗粒及其制备方法及其用途，以准确和广泛地诊断活性氧相关疾病。 构成：含水分散化学发光纳米粒子含有以下物质：含有疏水性化学发光物质的核心的0.01〜3重量％ 核心表面活性剂占0.5〜30wt％; 和67〜99.49重量％的水。 疏水化学发光物质的发光波长为650〜900nm。 疏水性化学发光物质由过氧草酸酯衍生物和荧光物质形成。 表面活性剂是泊洛沙姆。

公开(公告)号：KR1020100084039A

公开(公告)日：2010-07-23

申请号：KR1020090003431

申请日：2009-01-15

Applicant: 한국과학기술연구원

Inventor： 안희철 ,  안형준 ,  신동윤 ,  임종수 ,  안대로

IPC: C12N15/70 ,  C12N15/63 ,  C12N15/64 ,  C12N15/09

CPC classification number: C12N15/70 ,  C07K2319/35

Abstract: PURPOSE: A plasmid for expressing protein containing thioredoxin as a fusion partner is provided to easily remove thioredoxin and to massively produce a target protein. CONSTITUTION: A pET-32-S(-) plasmid contains a multi-cloning site for inserting thioredoxin gene, thrombin recognition site, S-tag coding region, and a gene encoding a target protein. A method for producing the target protein comprises: a step of inserting the gene encoding target protein into a multi-cloning site of a plasmid; a step of transforming the plasmid to E.coli and culturing; and a step of treating thrombine.

Abstract translation: 目的：提供用于表达含有硫氧还蛋白作为融合配偶体的蛋白质的质粒，以容易地除去硫氧还蛋白并大量产生靶蛋白。 构成：pET-32-S（ - ）质粒含有用于插入硫氧还蛋白基因，凝血酶识别位点，S标签编码区和编码靶蛋白的基因的多克隆位点。 制备靶蛋白的方法包括：将编码靶蛋白的基因插入质粒的多克隆位点的步骤; 将质粒转化大肠杆菌并培养的步骤; 和治疗血栓的步骤。