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公开(公告)号：KR1020080045910A
公开(公告)日：2008-05-26
申请号：KR1020060115164
申请日：2006-11-21
Abstract: A 3-hydroxybutyrate-co-3-hydroxyvalerate-co-lactate terpolymer, and a method for preparing the terpolymer are provided to use the terpolymer as a biodegradable polymer capable of replacing synthetic plastics. A 3-hydroxybutyrate-co-3-hydroxyvalerate-co-lactate terpolymer comprises lactate, 3-hydroxybutyrate and 3-hydroxyvalerate as a repeating unit. The preparation method comprises the step of cultivating or culturing the cell or plant having the gene of an enzyme which converts lactate into lactyl-CoA and converts 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA and the gene of a polyhydroxyalkanoate(PHA) synthetase.
Abstract translation: 提供3-羟基丁酸酯 - 共-3-羟基戊酸酯 - 共乳酸三元共聚物和制备三元共聚物的方法，以使三元共聚物作为能够代替合成塑料的可生物降解的聚合物。 3-羟基丁酸酯 - 共-3-羟基戊酸酯 - 共乳酸三元共聚物包括乳酸盐，3-羟基丁酸酯和3-羟基戊酸酯作为重复单元。 制备方法包括培养或培养具有将乳酸转化为乳酰辅酶A的酶的基因的细胞或植物，并将3-羟基链烷酸酯转化为3-羟基烷酰基-CoA和聚羟基链烷酸酯（PHA）合成酶的基因。
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公开(公告)号：KR1020080045908A
公开(公告)日：2008-05-26
申请号：KR1020060115162
申请日：2006-11-21
Abstract: A medium-chain-length 3-hydroxyalkanoate-lactate copolymer is provided to replace uses of the conventional synthetic plastics and to be applicable to medical fields. A medium-chain-length 3-hydroxyalkanoate-lactate copolymer contains lactate and 3-hydroxyalkanoate of medium chain length as repeating units. The 3-hydroxyalkanoate of medium chain length is selected from the group comprising 3-hydroxyhexanoate, 3-hydroxyheptanoate, 3-hydroxyoctanoate, 3-hydrononanoate, 3-hydroxydecanoate, 3-hydroxyundecanoate, and 3-hydroxydodecanoate. A method for preparing the copolymer includes a step of culturing or cultivating a cell or plant having a gene of an enzyme that coverts lactate into lactyl-CoA and 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA, and a polyhydroxyalkanoate synthase gene.
Abstract translation: 提供中链长度的3-羟基链烷酸酯 - 乳酸共聚物以代替常规合成塑料的用途并适用于医疗领域。 中链长3-羟基链烷酸酯 - 乳酸共聚物含有中链长度的乳酸盐和3-羟基链烷酸酯作为重复单元。 中链长度的3-羟基链烷酸酯选自3-羟基己酸酯，3-羟基庚酸酯，3-羟基辛酸酯，3-羟基壬酸酯，3-羟基癸酸酯，3-羟基十一酸酯和3-羟基十二酸酯。 制备共聚物的方法包括将具有将乳酸盐覆盖乳糖-CoA和3-羟基链烷酸酯的酶的基因的细胞或植物培养或培养成3-羟基烷酰基-CoA的多羟基烷酸酯合酶基因的步骤。
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公开(公告)号：KR1020080045906A
公开(公告)日：2008-05-26
申请号：KR1020060115160
申请日：2006-11-21
Abstract: A 3-hydroxybutyrate-4-hydroxybutyrate-lactate terpolymer is provided to be a biodegradable polymer and to replace the conventional synthetic plastics. A 3-hydroxybutyrate-4-hydroxybutyrate-lactate terpolymer contains lactate, 4-hydroxybutyrate, and 3-hydroxybutyrate as repeating units. A method for preparing the 3-hydroxybutyrate-4-hydroxybutyrate-lactate terpolymer includes a step of culturing or cultivating cells or plants having a gene of an enzyme, a phosphotransbutylase gene, a butyrate kinase gene, and a polyhydroxyalkanoate synthase gene. The enzyme coverts lactate into lactyl-CoA and converts 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA.
Abstract translation: 提供3-羟基丁酸酯-4-羟基丁酸酯 - 乳酸三元共聚物作为可生物降解的聚合物并代替常规的合成塑料。 3-羟基丁酸酯-4-羟基丁酸酯 - 乳酸三元共聚物含有乳酸盐，4-羟基丁酸盐和3-羟基丁酸盐作为重复单元。 制备3-羟基丁酸-4-羟基丁酸酯 - 乳酸三元共聚物的方法包括培养或培养具有酶基因，磷酸转苄二烯酸酶基因，丁酸酯激酶基因和聚羟基链烷酸酯合酶基因的细胞或植物的步骤。 酶将乳酸盐覆盖成乳酰CoA，并将3-羟基链烷酸酯转化为3-羟基烷酰基-CoA。
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公开(公告)号：KR1020080045905A
公开(公告)日：2008-05-26
申请号：KR1020060115159
申请日：2006-11-21
Abstract: A 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer is provided to replace the conventional synthetic plastics and to be usable in medical fields. A 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer contains lactate, 4-hydroxybutyrate, and 3-hydroxypropionate as repeating units. A method for preparing the 4-hydroxybutyrate-3-hydroxypropionate-lactate terpolymer includes a step of culturing or cultivating cells or plants having a gene of an enzyme that coverts lactate into lactyl-CoA and converts 3-hydroxyalkanoate into 3-hydroxyalkanoyl-CoA, a phosphotransbutylase gene, a butyrate kinase gene, and a polyhydroxyalkanoate synthase gene.
Abstract translation: 提供4-羟基丁酸-3-羟基丙酸酯 - 乳酸三元共聚物来替代常规的合成塑料并且可用于医疗领域。 4-羟基丁酸-3-羟基丙酸酯 - 乳酸三元共聚物含有乳酸盐，4-羟基丁酸盐和3-羟基丙酸盐作为重复单元。 制备4-羟基丁酸-3-羟基丙酸酯 - 乳酸三元共聚物的方法包括培养或培养具有将乳酸盐覆盖乳酸-CoA的酶基因的细胞或植物，并将3-羟基链烷酸酯转化为3-羟基烷酰基-CoA， 磷酸转铁蛋白酶基因，丁酸激酶基因和聚羟基链烷酸酯合酶基因。
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公开(公告)号：KR1020030070790A
公开(公告)日：2003-09-02
申请号：KR1020020010325
申请日：2002-02-26
Applicant: 한국과학기술원
IPC: C12N15/70
Abstract: PURPOSE: A recombinant bacterial system for producing MCL-PHA is provided, thereby mass-producing medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA). CONSTITUTION: A recombinant expression vector containing PHA synthesizing gene phaC, gene fadF, and/or gene fadD is provided, wherein the recombinant vector is p10499613C2. A transformant transformed with the recombinant expression vector p10499613C2 is provided, wherein the transformant is Escherichia coli WA101(p10499613C2), Escherichia coli WB101(p10499613C2), Escherichia coli WAB101(p10499613C2), Escherichia coli KA101(p10499613C2), Escherichia coli KB101(p10499613C2) or Escherichia coli KAB101(p10499613C2). A method for producing MCL-PHA comprises culturing a transformant selected from Escherichia coli WA101(p10499613C2), Escherichia coli WB101(p10499613C2), Escherichia coli WAB101(p10499613C2), Escherichia coli KA101(p10499613C2), Escherichia coli KB101(p10499613C2) or Escherichia coli KAB101(p10499613C2) in a medium containing fatty acid.
Abstract translation: 目的：提供用于生产MCL-PHA的重组细菌系统，从而大量生产中链长度聚（3-羟基链烷酸酯）（MCL-PHA）。 构成：提供含有PHA合成基因phaC，基因fadF和/或基因fadD的重组表达载体，其中重组载体为p10499613C2。 提供用重组表达载体p10499613C2转化的转化体，其中转化体是大肠杆菌WA101（p10499613C2），大肠杆菌WB101（p10499613C2），大肠杆菌WAB101（p10499613C2），大肠杆菌KA101（p10499613C2），大肠杆菌KB101（p10499613C2） 或大肠杆菌KAB101（p10499613C2）。 制备MCL-PHA的方法包括培养选自大肠杆菌WA101（p10499613C2），大肠杆菌WB101（p10499613C2），大肠杆菌WAB101（p10499613C2），大肠杆菌KA101（p10499613C2），大肠杆菌KB101（p10499613C2）或大肠杆菌 KAB101（p10499613C2）在含有脂肪酸的培养基中。
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Patent Agency Ranking
公开(公告)号：KR1020000051096A
公开(公告)日：2000-08-16
申请号：KR1019990001350
申请日：1999-01-19
Applicant: 한국과학기술원
CPC classification number: C12N15/70 , C12N9/0004 , C12R1/19
Abstract: PURPOSE: A recombinant coliform bacillus which is able to mass express the desulfurization-relating enzymes of Gordona sp. CYKS1 strain and a biological desulfurization method using thereof which lowers the cost of equipment and operation and is able to easily remove dibenzothiophene from organic sulfide. CONSTITUTION: A dszA gene of the Gordona sp. CYKS1 strain has a gene sequence of sequence No. 11 and a DszA protein of the Gordona sp. CYKS1 strain having an amino acid of sequence No. 12 derived from the dszA gene sequence. A dszB gene of the Gordona sp. CYKS1 strain has a gene sequence of sequence No. 13 and a DszB protein of the Gordona sp. CYKS1 strain having an amino acid of sequence No. 14 derived from the dszB gene sequence. A dszC gene of the Gordona sp. CYKS1 strain has a gene sequence of sequence No. 15 and a DszC protein of the Gordona sp. CYKS1 strain having an amino acid of sequence No. 16 derived from the dszA gene sequence. The biological desulfurization method comprises steps of: (i) inoculating a recombinant coliform bacillus of W3110/pTrcS1ExABC(Escherichia coli W3110/pTrcS1ExABC)(KCTC 05658BP) into a medium containing the organic sulfide; and (ii) cultivating the step (i) under aerobic condition.
Abstract translation: 目的：重组大肠杆菌，能够大规模表达Gordona sp。的脱硫相关酶。 CYKS1菌株和使用它的生物脱硫方法降低了设备和操作成本，并且能够容易地从有机硫化物中除去二苯并噻吩。 构成：Gordona sp。的dszA基因 CYKS1菌株具有序列号11的基因序列和Gordona sp的DszA蛋白。 具有源自dszA基因序列的序列号12的氨基酸的CYKS1菌株。 戈达纳菌属的dszB基因 CYKS1株具有序列号13的基因序列和Gordona sp的DszB蛋白。 具有源自dszB基因序列的序列号14的氨基酸的CYKS1菌株。 戈达纳氏菌的dszC基因 CYKS1菌株具有序列号15的基因序列和戈多纳菌属的DszC蛋白质。 具有源自dszA基因序列的序列号16的氨基酸的CYKS1菌株。 生物脱硫方法包括以下步骤：（i）将W3110 / pTrcS1ExABC（大肠杆菌W3110 / pTrcS1ExABC）（KCTC 05658BP）的重组大肠菌杆菌接种到含有机硫化物的培养基中; 和（ii）在需氧条件下培养步骤（i）。
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38.发明授权
公开(公告)号：KR101881342B1
公开(公告)日：2018-07-25
申请号：KR1020170094659
申请日：2017-07-26
Applicant: 한국과학기술원
Abstract: 본발명은자일로즈로부터폴리(락테이트-co-글라이콜레이트)와그 공중합체생산능을가지는재조합미생물에관한것으로더욱자세하게는, 외부로부터의글라이콜레이트프리커서를공급없이도폴리(락테이트-co-글라이콜레이트)와그 공중합체를생산하는능력을가지는재조합미생물및 이를이용한폴리(락테이트-co-글라이콜레이트) 공중합체의제조방법에관한것이다.
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39.发明授权
公开(公告)号：KR101774431B1
公开(公告)日：2017-09-05
申请号：KR1020160010549
申请日：2016-01-28
Applicant: 한국과학기술원
Abstract: 본발명은자일로즈로부터폴리(락테이트-co-글라이콜레이트)와그 공중합체생산능을가지는재조합미생물에관한것으로더욱자세하게는, 외부로부터의글라이콜레이트프리커서를공급없이도폴리(락테이트-co-글라이콜레이트)와그 공중합체를생산하는능력을가지는재조합미생물및 이를이용한폴리(락테이트-co-글라이콜레이트) 공중합체의제조방법에관한것이다.
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40.发明授权
公开(公告)号：KR101587618B1
公开(公告)日：2016-01-22
申请号：KR1020120142304
申请日：2012-12-07
Applicant: 한국과학기술원
CPC classification number: C12N9/0006 , C12N9/001 , C12N9/1025 , C12N9/93 , C12P7/42 , C12P7/52
Abstract: 본발명은 4-하이드록시부티릭산고생성능을가지는변이미생물및 이를이용한 4-하이드록시부티릭산의제조방법에관한것으로, 더욱상세하게는 4-하이드록시부티릭산의생성능을증가시키기위해숙신산으로부터 4-하이드록시부티릭산을합성하는유전자가증폭되어있고, 보충회로(anaplerotic pathway) 유전자가증폭되어있으며, 경쟁유전자들이결실되어있는 4-하이드록시부티릭산고생성능을가지는변이미생물및 이를미 호기성(micro-aerobic) 조건하에서배양하여 4-하이드록시부티릭산을고수율로제조하는방법에관한것이다. 본발명은 4-하이드록시부티릭산생성능을가지는변이미생물을제조하고, 이를이용함으로써, 4-하이드록시부티릭산을고수율로제조하는데유용하다.
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