公开(公告)号：KR1020140022082A

公开(公告)日：2014-02-21

申请号：KR1020137034430

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.

Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。

公开(公告)号：KR1020140022081A

公开(公告)日：2014-02-21

申请号：KR1020137034429

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: The present invention relates to novel hydrogenases separated from new hyperthermophilic strains belonging to Thermococcus genus, genes encoding the same, and a method for producing hydrogen using the same. According to a method for producing hydrogen of the present invention, a large amount of hydrogen can be produced by culturing the strains in a specific culture condition and therefore, it is more economical and effective compared to the existing method for producing hydrogen and hydrogen can be produced at a high temperature.

Abstract translation: 本发明涉及从属于Thermococcus属的新的超嗜热菌株分离出的新型氢化酶，以及使用该氢过氧化氢的方法。 根据本发明的氢的制造方法，可以通过在特定培养条件下培养菌株来生产大量氢，因此与现有的氢气制备方法相比，其更经济有效，氢可以是 在高温下生产。

公开(公告)号：KR1020130115496A

公开(公告)日：2013-10-22

申请号：KR1020120037823

申请日：2012-04-12

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  권개경 ,  임형순 ,  김윤재 ,  배승섭 ,  김태완

IPC: C12M1/107 ,  C12P3/00 ,  C01B3/02 ,  C12N1/20

CPC classification number: C12M21/04 ,  C01B3/02 ,  C12M23/36 ,  C12M23/42 ,  C12M23/58 ,  C12M29/26

Abstract: PURPOSE: A carbon monoxide dissolution device for producing hydrogen using anaerobic microorganisms is provided to help hydrogen-producing anaerobic microorganisms to effectively use carbon monoxides when hydrogen is produced, thereby enhancing hydrogen production efficiency. CONSTITUTION: A carbon monoxide dissolution device (100) for producing hydrogen using anaerobic microorganisms comprises: a main body (110) with an opening (111); tube cassettes (120) which have a tube for allowing a culture medium containing hydrogen-producing anaerobic microorganisms and carbon monoxides to pass; and a fitting member (130) which provides a delayed path for delaying time for dissolving the carbon monoxides. The tubes have supply ports (123) and outlets (124) by connecting the tubes between the tube cassettes. The both ends of the tubes are exposed to the outside to help the tube to be connected to other tubes. [Reference numerals] (230) Carbon monoxide supplying unit; (240) Hydrogen collecting unit; (250) Cooling unit; (260) Culture medium supplying unit

Abstract translation: 目的：提供一种使用厌氧微生物生产氢气的一氧化碳溶解装置，用于在生产氢气时帮助产氢厌氧微生物有效地使用一氧化碳，从而提高氢生产效率。 构成：使用厌氧微生物生产氢的一氧化碳溶解装置（100）包括：具有开口（111）的主体（110）; 管筒（120）具有用于允许含有产氢厌氧微生物和一氧化碳的培养基通过的管; 以及装配构件（130），其提供用于延迟用于溶解一氧化碳的时间的延迟路径。 管通过将管连接在管盒之间而具有供给口（123）和出口（124）。 管的两端暴露于外部，以帮助管连接到其他管。 （附图标记）（230）一氧化碳供给单元; （240）集氢单元; （250）冷却单元; （260）培养基供应单元

公开(公告)号：KR101223664B1

公开(公告)日：2013-01-17

申请号：KR1020110007943

申请日：2011-01-26

Applicant: 한국해양과학기술원 ,  재단법인 경북해양바이오산업연구원

Inventor： 우정희 ,  강성균 ,  이정현 ,  김상진 ,  박상이 ,  김준태 ,  김충곤

IPC: C12P7/40 ,  C12N15/55 ,  C12N15/63 ,  C12N1/21

Abstract: 본 발명은 신규한 나이트릴레이즈 및 이의 용도에 관한 것으로, 보다 구체적으로, 본 발명은 서열번호 1, 서열번호 3, 서열번호 5 및 서열번호 7로 이루어진 군에서 선택되는 아미노산 서열로 나타내는 나이트릴레이즈를 나이트릴 기질과 반응시키는 단계를 포함하는 카르복실산을 제조하는 방법, 및 상기 나이트릴레이즈를 포함하는 카르복실산 제조용 생촉매 조성물에 관한 것이다.

公开(公告)号：KR1020120086602A

公开(公告)日：2012-08-03

申请号：KR1020110007942

申请日：2011-01-26

Applicant: 한국해양과학기술원 ,  재단법인 경북해양바이오산업연구원

Inventor： 우정희 ,  강성균 ,  이정현 ,  김상진 ,  박상이 ,  김준태 ,  김충곤

IPC: C12P7/40 ,  C12N15/55 ,  C12N15/63 ,  C12N1/21

CPC classification number: C12P7/40 ,  C12N9/78 ,  C12N15/52 ,  C12Y305/05001

Abstract: PURPOSE: A novel nitrilase and a method for preparing carboxylic acid using the same are provided to enable biosynthesis of carboxylic acid with high efficiency. CONSTITUTION: A method for preapring carboxylic acid comprises: a step of preparing nitrilase VMN1 of sequence number 7 amino acid sequence or a biocatalyst composition containing nitrilase VMN1; and a step of reacting nitrilase VMN1 or the biocatalyst composition with nitrile substrate. The biocatalyst composition contains cells containing nitrilase VMN1 or cell culture or crushed material thereof. The nitrile substrate includes acetonitrile, acrylonitrile, adiponitrile, benzonitrile, butyronitrile, 4-chlorobenzonitrile, or mandelonitrile. A biocatalyst composition for preparing carboxylic acid contains nitrilase VMN1.

Abstract translation: 目的：提供一种新的腈水解酶和使用其制备羧酸的方法，以使得能够以高效率生物合成羧酸。 构成：用于预先羧酸的方法包括：制备序列号7氨基酸序列的腈水解酶VMN1或含有腈水解酶VMN1的生物催化剂组合物的步骤; 和腈水解酶VMN1或生物催化剂组合物与腈底物反应的步骤。 生物催化剂组合物含有含有腈水解酶VMN1或细胞培养物或其破碎材料的细胞。 腈底物包括乙腈，丙烯​​腈，己二腈，苄腈，丁腈，4-氯苄腈或扁桃腈。 用于制备羧酸的生物催化剂组合物含有腈水解酶VMN1。

公开(公告)号：KR1020110126012A

公开(公告)日：2011-11-22

申请号：KR1020100045692

申请日：2010-05-14

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  김상진 ,  권개경 ,  이현숙 ,  김윤재 ,  배승섭 ,  임재규 ,  전정호 ,  조요나 ,  황영옥 ,  차선신

IPC: C12N9/12 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70

CPC classification number: C12N9/12 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70

Abstract: PURPOSE: A DNA polymerase F7 and genes thereof are provided to enhance elongation and processivity and to be used in PCR. CONSTITUTION: A DNA polymerase F7 contains an amino acid sequence of sequence number 2. A DNA polymerase F7 gene encoding an amino acid sequence of sequence number 2 contains a base of sequence number 1. A recombinant vector contains the polymerase F7 gene of sequence number 1. A host cell is transformed with the recombinant vector containing the DNA polymerase F7 gene.

Abstract translation: 目的：提供DNA聚合酶F7及其基因以增加伸长和持续性，并用于PCR。 构成：DNA聚合酶F7含有序列号2的氨基酸序列。编码序列号2的氨基酸序列的DNA聚合酶F7基因含有序列号1的碱基。重组载体含有序列号1的聚合酶F7基因 用含有DNA聚合酶F7基因的重组载体转化宿主细胞。

公开(公告)号：KR1020110094092A

公开(公告)日：2011-08-19

申请号：KR1020117014737

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00 ,  C12N15/31

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus spp. and a method for producing hydrogen using the same are provided to produce hydrogen without high temperature and pressure. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 5. A gene encoding the hydrogenase contains a base sequence of sequence number 16. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium; and a step of isolating hydrogen. The medium contains carbon monoxide. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).

Abstract translation: 目的：从Thermococcus spp。分离出的新型氢化酶。 并且提供使用其制造氢的方法以在没有高温和高压的情况下产生氢。 构成：氢化酶含有序列号5的氨基酸序列。编码氢化酶的基因含有序列号16的碱基序列。从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在媒体中 和分离氢的步骤。 介质含有一氧化碳。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。

公开(公告)号：KR1020110093912A

公开(公告)日：2011-08-18

申请号：KR1020117014736

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  임형순 ,  김윤재 ,  배승섭 ,  임재규 ,  김민식 ,  김태완 ,  류지영 ,  정재연

IPC: C12N9/02 ,  C12N15/53 ,  C12P3/00 ,  C12N1/21

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52

Abstract: PURPOSE: A method for producing hydrogen using hydrogenase isolated from Thermococcus spp. is provided to cheaply and efficiently produce hydrogen even at high temperature. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 7. A gene encoding the hydrogenase contains a base sequence of sequence number 18. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium at 80°C under the anaerobic condition; and a step of isolating hydrogen. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains formate.

Abstract translation: 目的：使用从Thermococcus spp分离的氢化酶产生氢的方法。 即使在高温下也能廉价高效地生产氢。 构成：氢化酶含有序列号7的氨基酸序列。编码氢化酶的基因含有序列号18的碱基序列。一种从Thermococcus spp。生产氢的方法。 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在80℃的培养基中，在厌氧条件下; 和分离氢的步骤。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。 培养基含有甲酸。

公开(公告)号：KR1020110069744A

公开(公告)日：2011-06-23

申请号：KR1020107013071

申请日：2009-09-07

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  조요나 ,  김윤재 ,  배승섭 ,  임재규 ,  정인순

IPC: C12N9/02 ,  C12P3/00 ,  C12R1/01 ,  C12N9/04

CPC classification number: C12N9/0006 ,  C12N9/0067 ,  C12P3/00 ,  C12R1/01 ,  Y02P20/132 ,  Y02P20/52 ,  C12Y112/01004

Abstract: PURPOSE: A novel hydrogenase isolated from Thermococcus sp. and a method for producing hydrogen using the same are provided to produce a large amount of hydrogen even at high temperature. CONSTITUTION: A hydrogenase has an amino acid sequence selected from sequence numbers 1-8. A gene encoding the hydrogenase contains a base sequence selected from sequence numbers 12-19. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in a culture container; and a step of isolating hydrogen from culture container. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains carbon monoxide, alkyl formate, or starch. The culture is performed at 80°C and under anaerobic condition.

Abstract translation: 目的：从Thermococcus sp。 提供使用其制造氢的方法，即使在高温下也能产生大量的氢。 构成：氢化酶具有选自序列号1-8的氨基酸序列。 编码氢化酶的基因含有选自序列号12-19的碱基序列。 一种从Thermococcus spp。生产氢的方法 包括：制备介质的步骤; 培养热球菌属的一个步骤。 在文化容器中 以及从培养容器中分离氢的步骤。 热球菌属 是Thermococcus onnurineus NA1（保藏号：KCTC 10859BP）。 培养基含有一氧化碳，甲酸烷基酯或淀粉。 培养在80℃和无氧条件下进行。

公开(公告)号：KR1020110061837A

公开(公告)日：2011-06-10

申请号：KR1020090118351

申请日：2009-12-02

Applicant: 한국해양과학기술원

Inventor： 이정현 ,  강성균 ,  이현숙 ,  김상진 ,  권개경 ,  차선신 ,  전정호 ,  조요나 ,  김윤재

IPC: C12N9/14 ,  C12N15/55 ,  C12N15/63 ,  C12P1/00

CPC classification number: C12N9/14 ,  C12N15/67 ,  C12Y303/02009

Abstract: PURPOSE: A novel epoxide hydrolase is provided to hydrolyze non-degradable epoxide and to attenuate SO cytotoxicity. CONSTITUTION: An expoxide hydrolase has an amino acid sequence of sequence number 1. A method for preparing the epoxide hydrolase comprises: a step of inserting a gene encoding the epoxide hydrolase to an expression vector to prepare a recombinant vector; a step of introducing the recombinant vector to a host cell for overexpression; and a step of isolating and purifying the overexpressed apoxide hydrolase. The epoxy substrate is non-degradable epoxide substrate, styrene 7,8-oxide.

Abstract translation: 目的：提供一种新的环氧化物水解酶来水解不可降解的环氧化物并减弱SO细胞毒性。 一种环氧化物水解酶具有序列号1的氨基酸序列。制备环氧化物水解酶的方法包括：将编码环氧化物水解酶的基因插入表达载体以制备重组载体的步骤; 将重组载体导入宿主细胞以进行过表达的步骤; 以及分离和纯化过表达的过氧化物水解酶的步骤。 环氧基材是不可降解的环氧化物底物，苯乙烯7,8-氧化物。