Abstract:
PURPOSE: A novel hydrogenase isolated from Thermococcus spp. and a method for producing hydrogen using the same are provided to produce hydrogen without high temperature and pressure. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 5. A gene encoding the hydrogenase contains a base sequence of sequence number 16. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium; and a step of isolating hydrogen. The medium contains carbon monoxide. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP).
Abstract:
PURPOSE: A method for producing hydrogen using hydrogenase isolated from Thermococcus spp. is provided to cheaply and efficiently produce hydrogen even at high temperature. CONSTITUTION: A hydrogenase contains an amino acid sequence of sequence number 7. A gene encoding the hydrogenase contains a base sequence of sequence number 18. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in the medium at 80°C under the anaerobic condition; and a step of isolating hydrogen. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains formate.
Abstract:
PURPOSE: A novel hydrogenase isolated from Thermococcus sp. and a method for producing hydrogen using the same are provided to produce a large amount of hydrogen even at high temperature. CONSTITUTION: A hydrogenase has an amino acid sequence selected from sequence numbers 1-8. A gene encoding the hydrogenase contains a base sequence selected from sequence numbers 12-19. A method for producing hydrogen from Thermococcus spp. comprises: a step of preparing a medium; a step of culturing Thermococcus spp. in a culture container; and a step of isolating hydrogen from culture container. The Thermococcus spp. is Thermococcus onnurineus NA1(deposit number: KCTC 10859BP). The medium contains carbon monoxide, alkyl formate, or starch. The culture is performed at 80°C and under anaerobic condition.
Abstract:
PURPOSE: A novel epoxide hydrolase is provided to hydrolyze non-degradable epoxide and to attenuate SO cytotoxicity. CONSTITUTION: An expoxide hydrolase has an amino acid sequence of sequence number 1. A method for preparing the epoxide hydrolase comprises: a step of inserting a gene encoding the epoxide hydrolase to an expression vector to prepare a recombinant vector; a step of introducing the recombinant vector to a host cell for overexpression; and a step of isolating and purifying the overexpressed apoxide hydrolase. The epoxy substrate is non-degradable epoxide substrate, styrene 7,8-oxide.