公开(公告)号：JPS6237159A

公开(公告)日：1987-02-18

申请号：JP17574185

申请日：1985-08-12

Applicant: AGENCY IND SCIENCE TECHN ,  MITSUBISHI MONSANTO CHEM

Inventor： KODAMA AKIRA ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  MIYASHITA KAZUHISA

IPC: B32B27/30

公开(公告)号：JPS61260885A

公开(公告)日：1986-11-19

申请号：JP10087885

申请日：1985-05-13

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  FURUSAWA KIYOTAKA ,  KOKUBU TOMOKUNI ,  TSUDA KEISHIRO

IPC: C12N15/09 ,  C07K14/70 ,  C12N1/20 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To construct a specific recombinant plasmid having a specific DNA sequence, by making it possible to cut out a DNA sequence coding leucine enkephalin consisting of 28 base pairs by cleavage with a restriction enzyme EcoRI. CONSTITUTION:A novel recombinant plasmid pLEG1 obtained by carrying out sorting out of a plasmid vector and chemical synthesis of a DNA coding leucine enkephalin, and passing the synthesized DNA through processes of integration into a plasmid vector, introduction of the resultant recombinant plasmid into Escherichia coli K12C600 strain, separation of microbial cells having the recombinant plasmid and separation of the plasmid. DNAs expressed by formulas I and II synthesized by the phosphoramidite solid phase synthesis method may be used as the DNA coding the leucine enkephalin.

公开(公告)号：JPS63267276A

公开(公告)日：1988-11-04

申请号：JP31283686

申请日：1986-12-26

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  TSUDA KEISHIRO

IPC: C12N15/00 ,  C12N1/20 ,  C12N1/21 ,  C12N15/09 ,  C12N15/70 ,  C12R1/19

Abstract: PURPOSE:To provide a novel recombinant plasmid pTP70-1 containing a gene obtained by modifying a dihydro-folate reductase gene originated from E.coli, having a specific DNA sequence and capable of facilitating the use of dihydro- folate reductase gene as a trimethoprim-resistance marker. CONSTITUTION:The novel recombinant plasmid pTP70-1 is stably replicable in E.coli, capable of imparting the host E.coli with trimethoprim resistance and ampicillin resistance, has a size of 4608 base pairs and is composed of a specific DNA sequence (omitted). E.coli K12C600 strain containing pTP70-1 has been deposited in the Fermentation Research Institute in the name of FERM P-9091.

公开(公告)号：JPS63245680A

公开(公告)日：1988-10-12

申请号：JP7937887

申请日：1987-03-31

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  KOKUBU TOMOKUNI ,  FURUSAWA KIYOTAKA ,  OHASHI SHINICHI ,  TSUDA KEISHIRO

IPC: C12N15/09 ,  C07H21/04 ,  C07K14/655 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To readily give a fused protein measurable enzymatic activity by making up a novel recombined plasmid pGIF1 including a gene coding fused protein from dehydrofolate reductase and somastatin to solubilize the fused protein. CONSTITUTION:The recombinant plasmid pGIF1 is stably copied in E. coli and gives the host E. coli, resistance to trimethoprim and ampicillin. In the plasmid, the gene giving trimethoprim resistance is recombined by modifying a part of the 3'-terminal of dihydrofolate reductase in Bacillus subtilis to code the fused protein with 4789 pairs of bases. The recombinant plasmid pGIF1 is introduced into E. coli C600 strain and the transformed strain is deposited as FERM P-9301.

公开(公告)号：JPS63102698A

公开(公告)日：1988-05-07

申请号：JP24925986

申请日：1986-10-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  KOKUBU TOMOKUNI ,  FURUSAWA KIYOTAKA ,  OHASHI SHINICHI ,  TSUDA KEISHIRO

IPC: C12N15/09 ,  C07K1/12 ,  C07K14/00 ,  C07K14/575 ,  C07K14/655 ,  C07K14/70 ,  C07K19/00 ,  C12N1/20 ,  C12N9/06 ,  C12N15/00 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To make it possible to produce leucine enkephalin, by separating dehydrofolate reductase-leucine enkephalin fusion protein produced by E.coli integrated with a specific plasmid from the other substances in a medium, purifying and decomposing by cyanogen bromide method. CONSTITUTION:Dehydrofolate reductase-leucine enkephalin fusion protein is separated from a medium in which E.coli integrated with plasmid pBSFOLEK1 by ion exchange column chromatography and gel filtration chromatography and purified. Then the protein is decomposed by cyanogen bromide decomposition method and leucine enkephalin is separated and purified by reverse phase high performance liquid chromatography.

公开(公告)号：JPS63102696A

公开(公告)日：1988-05-07

申请号：JP24926086

申请日：1986-10-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： IWAKURA MASAHIRO ,  KOKUBU TOMOKUNI ,  FURUSAWA KIYOTAKA ,  OHASHI SHINICHI ,  TSUDA KEISHIRO

IPC: C12N15/09 ,  C07K1/16 ,  C07K14/575 ,  C07K14/655 ,  C07K14/70 ,  C07K19/00 ,  C12N9/06 ,  C12N15/00 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19

Abstract: PURPOSE:To obtain a dihydrofolate reductase-leucine enkephalin fusion protein which is protein produced by Escherichia coli integrated with a specific plasmid and a raw material for leucine enkephalin having nonhabitual morphine-like analgesic action. CONSTITUTION:Escherichia coli integrated with plasmid pBSFOLEK1 is cultivated to produce dihydrofolate reductase-leucine enkephalin fusion protein having an amino acid sequence shown by the fig. The cell is separated from the culture solution, removed, the culture solution is subjected to ion exchange chromatography and then the aimed fusion protein is separated and purified by using dihydrofolate reductase as a standard while passing through the solution gel filtration chromatography.

公开(公告)号：JPS62221467A

公开(公告)日：1987-09-29

申请号：JP6300086

申请日：1986-03-20

Applicant: AGENCY IND SCIENCE TECHN

Inventor： SORA TATSUO ,  MAEDA HIDEATSU ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

IPC: B05D1/18 ,  B05D7/24 ,  B41M5/26

Abstract: PURPOSE:To efficiently form a highly homogenous membrane layer, by treating the surface of a substrate with a coupling agent and forming a purple membrane-containing membrane layer due to the polymerization of acrylamide to the treated surface. CONSTITUTION:A purple membrane is the one isolated from Halobacterium halobium being one kind of halophil and contains bacteriorhodopsin being chromoprotein and is dissolved in an aqueous solution containing 5-15wt% of acrylamide to obtain a desired acrylamide solution. A substrate such as a glass substrate, an ITO electrode substrate or a metal plate is treated with a silane coupling agent and acrylamide is polymerized while the acrylamide solution containing the purple membrane is contacted with the surface of the surface treated substrate to form a homogenous membrane layer of a purple membrane-containing acrylamide gel.

公开(公告)号：JPS62126984A

公开(公告)日：1987-06-09

申请号：JP26822085

申请日：1985-11-28

Applicant: AGENCY IND SCIENCE TECHN

Inventor： WAKURA MASAHIRO ,  TSUDA KEISHIRO

IPC: C12N15/09 ,  C12N1/20 ,  C12N15/70 ,  C12Q1/68 ,  C12R1/19 ,  G01N33/50

Abstract: PURPOSE:The titled plasmid useful for microbial industries, biochemical industries and medical uses, having specific DNA sequence, number of base pairs and ampicillin resistance. CONSTITUTION:Plasmid pTP64-41 derived from plasmid pTP63-21 is blended with plasmid pYEJ001, treated with restriction enzyme HindIII and then reacted with T4-DNA ligase to give a bonded mixture. Then the mixture is transduced into Escherichia coli K12C600 strain to give a transformant strain having resistance to trimethoprim, ampicillin and chloramphenicol. Then a plasmid is separated from the transformant strain to give recombinant plasmid pTP68-1 which provides a host with resistance to ampicillin, trimethoprim, chloramphenicol, etc., has size of 6096 bases and DNA sequence such as DNA sequence shown by the formula.

公开(公告)号：JPS6297686A

公开(公告)日：1987-05-07

申请号：JP23740485

申请日：1985-10-25

Applicant: AGENCY IND SCIENCE TECHN ,  HITACHI LTD

Inventor： ASAI MICHIHIKO ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  TAKEMOTO KAZUNARI ,  KITO MAKOTO ,  KUROIWA MINORU ,  KOMATSU TAKASHI

IPC: C02F1/00 ,  C02F1/32 ,  C02F1/42 ,  C02F1/44 ,  C02F9/00 ,  C08J7/00

Abstract: PURPOSE:To reduce the elution of an org. substance, by forming a plasma polymerization layer to the inside surface of each of the piping between apparatuses and the piping up to an area using ultrapure water. CONSTITUTION:Primary pure water Wa supplied is subjected to sterilization treatment due to the irradiation of ultraviolet rays in an ultraviolet sterilizing apparatus 1 and introduced into an ion exchange apparatus 2 through piping 4A to remove the dissolved ion in the primary pure water Wa. The primary pure water Wa after the removal of the ion is introduced into an ultrafiltration apparatus 3 through piping 4B and fine particles contained in the primary pure water Wa are removed to obtain ultrapure water Wb which is, in turn, supplied to a use area through piping 4C. A plasma polymerization layer 6 comprising a monomer such as hydrocarbon, a nitrogen-containing compound and a fluorine- containing compound is formed to the piping main body of each of the pipings 4A-4C. By this method, ultrapure water is obtained.

公开(公告)号：JPS6246232A

公开(公告)日：1987-02-28

申请号：JP18523785

申请日：1985-08-23

Applicant: AGENCY IND SCIENCE TECHN

Inventor： HADO MASAKATSU ,  SAKAI TSUKASA ,  TSUDA KEISHIRO

IPC: G01N13/00

Abstract: PURPOSE:To automatically and continuously measure the advance or retraction contact angle between specimens, by performing the limitation of the potential between a solid specimen and a liquid specimen and restricting the advance or retraction condition of the meniscus between both specimens. CONSTITUTION:The titled apparatus is constituted of an electric balance 1 having a mass measuring terminal part 2, a liquid surface minute speed moving mechanism 13, a measuring cell 5 having a cover 8 mounted to the upper part thereof and receiving a liquid specimen and a recorder 7 for continuously and automatically recording the output of the balance 1. Because a solid specimen 4 is electrically contacted with a hook 3, the solid specimen 4 is used as an acting electrode and an opposed electrode 9 and a standard electrode 11 are connected to a usual potentiostat 15 to regulate the potential of the solid specimen 4 according to a three-electrode method. When the plate 14 having the measuring cell 5 mounted thereto is electrically raised or fallen at a constant speed by the moving mechanism 13, a contact angle can be easily measured while the meniscus formed between the solid specimen 4 and the liquid specimen 6 is held to the state of an advance or retraction contact angle.