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    VASCULAR EMBOLIC AGENT
    1.
    发明专利
    VASCULAR EMBOLIC AGENT 失效

    公开(公告)号：JPH07330618A

    公开(公告)日：1995-12-19

    申请号：JP14553394

    申请日：1994-06-03

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： ITO SHOJI ,  MATSUMARU YUJI ,  HIRANO TAKASHI ,  OHASHI SHINICHI

    IPC: A61K31/785 ,  A61K49/00 ,  A61P9/00

    Abstract: PURPOSE:To obtain a vascular embolic agent, excellent in the safety such as neither adverse side effect nor danger caused by organic solvents and suitably usable in intravascular operation. CONSTITUTION:This vascular embolic agent comprises an aqueous solution containing a heat-sensitive polymeric compound, constituted of at least one recurring unit of an N-substituted (meth)acrylamide represented by the formula (R is H or methyl; R is H, an alkyl or an alkoxyalkyl; R is an alkyl or an alkoxvalkyl) and having a molecular weight corresponding to 0.01-6.0dl/g intrinsic viscosity [eta] in a tetrahydrofuran solution at 27 deg.C and 10-37 deg.C transition temperature in water at 0.5-50wt.% concentration. A homopolymer of the N- substituted (meth)acrylamide, a copolymer of two or more thereof and a copolymer of the N-substituted (meth)acrylamide and other monomers are cited as the heat-sensitive polymeric compound. The vascular embolic agent is capable of emerging from a catheter, thereby increasing the temperature, convertible into a solid form and blocking blood vessels.

    NOVEL RECOMBINANT PLASMID PGRF44-22
    2.
    发明专利
    NOVEL RECOMBINANT PLASMID PGRF44-22 失效

    公开(公告)号：JPH02142480A

    公开(公告)日：1990-05-31

    申请号：JP29420388

    申请日：1988-11-21

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  TANAKA YOSHIO

    IPC: C12N1/21 ,  C07K14/00 ,  C07K14/575 ,  C07K14/60 ,  C07K19/00 ,  C12N15/09 ,  C12N15/16 ,  C12N15/62 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19

    Abstract: NEW MATERIAL:A plasmid pGRF44-22, stably replicative in Escherichia coli and capable of imparting trimethoprim and ampicillin tolerance to host Escherichia coli and encoding a fused protein of a derivative of a bovine growth hormone releasing factor having an amino acid sequence expressed by the formula to a dihydrofolate reductase. USE:For producing a bovine growth hormone releasing factor(GRF) and dihydrofolate reductase(DHFR). PREPARATION:For example, a DNA fragment prepared by cleaving a plasmid pMEK2 for producing leucine enkephalin with a restriction enzyme is linked to a DNA fragment obtained by cleaving a recombinant plasmid pGRF2-15 capable of coding DHFR and an amino acid sequence of the 1st to 29th residues of a bovine GRF using a T4-DNA ligase, inserted into Escherichia coli and cultured to select the resultant colony. Thereby, the objective pGRF44-22 is obtained.

    HIGH SENSITIVITY DETECTION OF PROTEIN
    5.
    发明专利
    HIGH SENSITIVITY DETECTION OF PROTEIN 失效

    公开(公告)号：JPS62228168A

    公开(公告)日：1987-10-07

    申请号：JP5181586

    申请日：1986-03-10

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： YOKOYAMA NAOKI ,  NAKAO YUKIMICHI ,  OHASHI SHINICHI ,  KAERIYAMA KYOJI ,  TSUDA KEISHIRO ,  SUDA MASAO ,  IMAI TOMOYUKI

    IPC: G01N31/00 ,  G01N33/68

    Abstract: PURPOSE:To safely and economically detect protein immobilized on a membrane with high sensitivity, by a simple means wherein the membrane having protein immobilized thereon is treated with a palladium colloid solution with predetermined pH stabilized by a surfactant and a base metal is applied to the treated membrane by an electroless plating. CONSTITUTION:A membrane such as a nitrocellulose having protein immobilized thereon is treated with a palladium colloid solution with pH 5 or less stabilized by an anionic surfactant such as sodium dodecylbenzenesulfonate and a base metal is subsequently applied to the treated membrane by electroless plating. The content of palladium in the palladium colloid solution is 0.01-30mmol/l and the content of the surfactant is 0.001-5wt%. The pH of said colloid solution is pref. adjusted by an acid such as acetic acid or hydrochloric acid. The membrane having protein immobilized thereon is immersed in the palladium colloid solution to be stained and, thereafter, the electroless plating of the base metal is applied to said membrane to enhance detection sensitivity.

    NOVEL RECOMBINANT PLASMID HAVING SPECIFIC SEQUENCE
    6.
    发明专利
    NOVEL RECOMBINANT PLASMID HAVING SPECIFIC SEQUENCE 失效

    公开(公告)号：JPS62115287A

    公开(公告)日：1987-05-26

    申请号：JP25566085

    申请日：1985-11-14

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  FURUSAWA KIYOTAKA ,  OHASHI SHINICHI ,  TSUDA KEISHIRO

    IPC: C12N15/09 ,  C07H21/04 ,  C07K14/60 ,  C12N1/20 ,  C12N1/21 ,  C12P21/02 ,  C12R1/19

    Abstract: PURPOSE:A recombinant plasmid containing a gene to code 1st-29th peptide fragments among bovine growth hormone release factor peptide having secretion adjusting activity of growth hormone. CONSTITUTION:Novel recombinant plasmid pGRF29-28 consisting of 5406 pairs of bases having DNA sequence partially shown by formula II, a recombinant plasmid which can provide a host with ampicillin resistance, codes a peptide wherein a peptide sequence consisting of 6 amino acids of methionine-isoleucine- isoleucine-glutamic acid-glycine-alginine is fused to an amino end side of 1st-29th peptide fragment among bovine growth hormone release factor peptide by cleavage with restriction enzyme BclI and can free a DNA sequence consisting of 107 pairs of bases shown by formula I.

    NEW DIHYDROFOLATE REDUCTASE AND MODIFIED DIHYDROFOLATE REDUCTASE GENE
    7.
    发明专利
    NEW DIHYDROFOLATE REDUCTASE AND MODIFIED DIHYDROFOLATE REDUCTASE GENE 失效

    公开(公告)号：JPH05146291A

    公开(公告)日：1993-06-15

    申请号：JP33623691

    申请日：1991-11-26

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  KOKUBU TOMOKUNI ,  OHASHI SHINICHI

    IPC: C12N9/06 ,  C12N11/00 ,  C12N15/09 ,  C12N15/53 ,  C12R1/19

    Abstract: PURPOSE:To obtain the subject enzyme capable of suppressing inactivation of the enzyme and providing a homogenous immobilized enzyme having high activity by transducing an amino acid sequence containing plural amino acid residues having cysteine residue as the carboxyl terminal into the carboxyl terminal side of an enzymic protein. CONSTITUTION:A genetic DNA capable of coding a dihydrofolate reductase is used to replace the sequence part capable of coding the carboxyl terminal side of this enzymic protein with a chemical synthetic DNA. Thereby, a genetic DNA capable of coding a modified enzymic protein in which a short amino acid sequence is transduced so that the carboxyl terminal may be the cysteine residue is prepared. The resultant genetic DNA is then integrated into a vector such as a plasmid for expression to construct an expression vector, which is subsequently transduced into a host such as Escherichia coli and expressed. The obtained modified enzymic protein can be separated and purified from the host microbial cell to afford a new dihydrofolate reductase, having an amino acid sequence expressed by the formula, capable of efficiently producing a homogenous immobilized enzyme having high activity and utilizable for producing various useful substances.

    GROWTH HORMONE-RELEASING FACTOR DERIVATIVE
    10.
    发明专利
    GROWTH HORMONE-RELEASING FACTOR DERIVATIVE 失效

    公开(公告)号：JPH02258799A

    公开(公告)日：1990-10-19

    申请号：JP7946289

    申请日：1989-03-30

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  OBARA KAZUHIKO ,  KOKUBU TOMOKUNI ,  OHASHI SHINICHI

    IPC: C12P21/02 ,  A61K38/04 ,  C07K1/20 ,  C07K1/22 ,  C07K14/00 ,  C07K14/575 ,  C07K14/60 ,  C07K19/00 ,  C12N15/09 ,  C12N15/18 ,  C12N15/62 ,  C12R1/19

    Abstract: NEW MATERIAL:A dihydrofolate reductase-growth hormone-releasing factor derivative fused protein produced by Escherichia coli containing recombinant pGRF2-15. USE:An agent for fermentation, stock raising and drugs having bovine growth hormone-releasing factor and dihydrofolic acid reducing activity. PREPARATION:For example, recombinant plasmid pGRF2-15 containing a base sequence to code amino acid sequence of fused protein comprising dihydrofolate reductase-growth hormone-releasing factor shown by the formula is inserted into Escherichia coil, transformed, the transformant is cultured in a medium, the cell is ground, centrifuged, a fraction of supernatant liquid is collected and treated with streptomycin sulfate and ammonium sulfate to give an enzyme solution. The solution is purifying by using mesotrixate bonded affinity column chromatography to give growth hormone-releasing factor derivative.

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