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    DIHYDROFOLIC ACID REDUCTASE
    4.
    发明专利
    DIHYDROFOLIC ACID REDUCTASE 失效

    公开(公告)号：JPH01144974A

    公开(公告)日：1989-06-07

    申请号：JP30215287

    申请日：1987-11-30

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  SAKAI TSUKASA ,  TANAKA YOSHIO

    IPC: C12N1/20 ,  C12N9/06 ,  C12N15/09 ,  C12R1/19

    Abstract: PURPOSE:To easily purify the title enzyme useful for the production of enzyme and medicine, in high purity, by culturing E.coli and purifying the cell-free extract of the cultured product with methotrexate-bonded affinity chromatography. CONSTITUTION:An E.coli containing plasmid pT64-1 is cultured and the cell-free extract of the cultured bacterial cell is treated with an ion-exchange column and purified by methotrexate-bonded affinity chromatography and anion exchange chromatography to obtain the objective enzyme.

    SYSTEM FOR MAKING ULTRAPURE WATER
    6.
    发明专利
    SYSTEM FOR MAKING ULTRAPURE WATER 失效

    公开(公告)号：JPS6297687A

    公开(公告)日：1987-05-07

    申请号：JP23740585

    申请日：1985-10-25

    Applicant: AGENCY IND SCIENCE TECHN ,  HITACHI LTD

    Inventor： ASAI MICHIHIKO ,  SAKAI TSUKASA ,  TSUDA KEISHIRO ,  TAKEMOTO KAZUNARI ,  KITO MAKOTO ,  KUROIWA MINORU ,  KOMATSU TAKASHI

    IPC: C02F1/00 ,  C02F1/32 ,  C02F1/42 ,  C02F1/44 ,  C02F9/00 ,  C08J7/00

    Abstract: PURPOSE:To reduce the elution of an org. substance, by applying plasma treatment to the inside surface of each of the piping between apparatuses and the piping up to an area using ultrapure water to form a modified layer to said inside surface. CONSTITUTION:Primary pure water Wa supplied is subjected to sterilization treatment in an ultraviolet sterilization apparatus 1 and introduced into an ion exchange apparatus 2 through piping 4A to remove the dissolved ion in the pure water Wa. The pure water Wa after the removal of the ion is introduced into an ultrafiltration apparatus 3 through piping 4B and fine particles in the pure water Wa are removed to obtain ultrapure water Wb which is, in turn, supplied to a use area through piping 4C. Plasma treatment is applied to the inner surface of the piping main body 5 of each of the pipings 4A-4C by Ar-gas to form a modified layer 6. By this method, ultrapure water can be obtained.

    ANGIOTENSIN I
    9.
    发明专利
    ANGIOTENSIN I 失效

    公开(公告)号：JPH01252287A

    公开(公告)日：1989-10-06

    申请号：JP7967888

    申请日：1988-03-31

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： IWAKURA MASAHIRO ,  OHASHI SHINICHI ,  SAKAI TSUKASA ,  TANAKA YOSHIO

    IPC: C12N15/09 ,  C07K1/14 ,  C07K1/22 ,  C07K7/14 ,  C07K14/00 ,  C07K19/00 ,  C12N1/20 ,  C12N1/21 ,  C12N9/06 ,  C12P21/02 ,  C12R1/19

    Abstract: PURPOSE:To obtain a large amount of dihydrofolic acid reductase-angiotensin fused protein, by manifesting a fused gene in which an angiotensin I gene is included in a recombined plasmid pLEK1 with a colon bacillus. CONSTITUTION:A newly recombined plasmid pANG1-23, which is replicated in stable with a colon bacillus, imparting resistance to trimethoprim and resistance to ampicillin to a colon bacillus as a host, having a size of 4222 basic pairs and having a replaced structure with a conformation of 28 basic pairs containing a conformation coding leucine enkephaline between BamHI site and MluI site in pLEK1 to a chemically synthesized DNA of 43 basic pairs containing a conformation coding angiotensin I, is produced. A colon bacillus introduced of pANG1-23 produces a large amount of dihydrofolic acid reductase- angiotensin fused protein.

    DEPROTECTION OF CYCLIC SILICON PROTECTING GROUP
    10.
    发明专利
    DEPROTECTION OF CYCLIC SILICON PROTECTING GROUP 失效

    公开(公告)号：JPH01230594A

    公开(公告)日：1989-09-14

    申请号：JP5717288

    申请日：1988-03-10

    Applicant: AGENCY IND SCIENCE TECHN

    Inventor： FURUSAWA KIYOTAKA ,  SAKAI TSUKASA ,  TANAKA YOSHIO

    IPC: C07H19/06 ,  C07H1/00 ,  C07H19/16 ,  C07H23/00

    Abstract: PURPOSE:To eliminate protecting groups completely under mild conditions in a shortened time, being very beneficial in nucleic acid synthesis, by allowing a trialkylammonium fluoride to act on specific cyclic silicon-protecting derivatives. CONSTITUTION:(A) Trialkylammonium fluoride is allowed to act on (B) cyclic silicon-protecting groups of formula I (R, R' are alkyl, cycloalkyl, aryl, aralkyl, alkaryl; R'' is H, hydroxyl, protected hydroxyl; B is monovalent residue of nucleic acid base or its derivative to effect hydrolysis whereby the compound of formula II is obtained.

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