公开(公告)号：SG10201605049QA

公开(公告)日：2016-07-28

申请号：SG10201605049Q

申请日：2012-05-21

Applicant: FLUIDIGM CORP

Inventor： ANDERSON MEGAN ,  CHEN PEILIN ,  FOWLER BRIAN ,  JONES ROBERT C ,  KAPER FIONA ,  LEBOFSKY RONALD ,  ANDREW MAY

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

公开(公告)号：SG11201504876TA

公开(公告)日：2015-07-30

申请号：SG11201504876T

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN

IPC: C12M1/22

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment. A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

公开(公告)号：SG10201404683TA

公开(公告)日：2014-10-30

申请号：SG10201404683T

申请日：2009-12-07

Applicant: FLUIDIGM CORP

Inventor： FOWLER BRIAN

公开(公告)号：CA2895638A1

公开(公告)日：2014-09-18

申请号：CA2895638

申请日：2014-03-14

Applicant: FLUIDIGM CORP

Inventor： WEST JASON A A ,  FOWLER BRIAN

IPC: C12Q1/02 ,  C12M1/12 ,  C12M1/34 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/68

Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

公开(公告)号：CA2480728A1

公开(公告)日：2003-10-16

申请号：CA2480728

申请日：2003-04-01

Applicant: FLUIDIGM CORP

Inventor： MANGER IAN D ,  THRONDSET WILLIAM ,  NASSEF HANY RAMEZ ,  NORTON PIERCE ,  FARRELL KEVIN ,  FOWLER BRIAN ,  HAO CUNSHENG CASEY ,  LIAU YISH-HANN ,  JAVADI SHERVIN ,  DARIDON ANTOINE ,  CHOU HOU-PU

IPC: G01N37/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12N1/00 ,  C12Q1/02 ,  G01N15/02 ,  G01N15/14

Abstract: The invention provides systems (2000), including microfluidic mechanisms, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. These mechanisms may enable controlle d input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any number of suitable times in the system to allow particles to be sorted, cultured, mixed, treate d, and/or assayed, among others. These combinations may allow the response of particles to treatment to be measured on a shorter time scale than was previously possible. Therefore, systems of the invention may allow a broad range of cell and particle assays, such as drug screens, cell characterizations, research studies, and/or clinical analyses, among others, to be scaled down to microfluidic size. Such scaled-down assays may use less sample and reagent, may be less labor intensive, and/or may be more informative than comparable macrofluidic assays.