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    UNIVERSAL PROBE ASSAY METHODS
    1.
    发明申请
    UNIVERSAL PROBE ASSAY METHODS 审中-公开
    通用检测方法

    公开(公告)号：WO2012054933A3

    公开(公告)日：2012-06-28

    申请号：PCT/US2011057536

    申请日：2011-10-24

    Applicant: FLUIDIGM CORP ,  LIVAK KENNETH ,  WEST JASON A A ,  JONES ROBERT C

    Inventor： LIVAK KENNETH ,  WEST JASON A A ,  JONES ROBERT C

    IPC: C12P19/34

    CPC classification number: C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    Abstract: Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5' to the target sequence, and a second probe-binding sequence 3' to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    Abstract translation: 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物 。

    METHODS AND DEVICES FOR ANALYSIS OF DEFINED MULTICELLULAR COMBINATIONS
    3.
    发明专利
    METHODS AND DEVICES FOR ANALYSIS OF DEFINED MULTICELLULAR COMBINATIONS 未知

    公开(公告)号：SG10201707485YA

    公开(公告)日：2017-10-30

    申请号：SG10201707485Y

    申请日：2014-03-14

    Applicant: FLUIDIGM CORP

    Inventor： WEST JASON A A ,  FOWLER BRIAN

    Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment. A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    4.
    发明专利
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 未知

    公开(公告)号：CA2878787A1

    公开(公告)日：2013-09-06

    申请号：CA2878787

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    IPC: G01N15/10

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    INTEGRATED SINGLE CELL SEQUENCING
    6.
    发明专利
    INTEGRATED SINGLE CELL SEQUENCING 未知

    公开(公告)号：SG11201609055RA

    公开(公告)日：2016-11-29

    申请号：SG11201609055R

    申请日：2015-05-08

    Applicant: FLUIDIGM CORP

    Inventor： WEST JASON A A ,  FOWLER BRIAN ,  CHARN TZE-HOWE ,  JOHNSON CHRISTIAN F ,  UNGER MARC A

    IPC: C12Q1/68

    Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    7.
    发明专利
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 未知

    公开(公告)号：SG11201405235VA

    公开(公告)日：2014-11-27

    申请号：SG11201405235V

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    IPC: G01N15/10

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS
    8.
    发明公开
    METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS 有权
    方法，系统和设备采集和处理几个单独的细胞的微流控芯片

    公开(公告)号：EP2820394A4

    公开(公告)日：2015-07-08

    申请号：EP13754093

    申请日：2013-02-28

    Applicant: FLUIDIGM CORP

    Inventor： FOWLER BRIAN ,  KIMBALL JAKE ,  MAUNG MYO THU ,  MAY ANDREW ,  NORRIS MICHAEL C ,  TOPPANI DOMINIQUE G ,  UNGER MARC A ,  WANG JING ,  WEST JASON A A

    IPC: G01N15/10

    CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 方法，系统和装置被描述为多个单细胞捕获和处理利用微流体。 提供了用于捕获，分区和/或个体细胞从细胞中产生地连同遗传信息和/或与每个单独的电池反应的更大的人口操纵的工具和技术。 不同的捕获配置可以被用于捕获个人的细胞，然后在处理的多室反应配置每个单独的小区。 一些实施例可以提供特定目标扩增，全基因组扩增，全转录扩增，实时PCR制备，拷贝数变异，预扩增，测序mRNA和/或多个单独的电池单元型thathave是来自于较大的人口划分 细胞。 一些实施例可以提供用于其它应用。 一些实施例可以被配置为单个细胞或相关联的反应产物进行成像作为处理的一部分。 反应产物可以收获和/或在某些情况下，进一步的分析。

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