公开(公告)号：CA2387283C

公开(公告)日：2008-08-19

申请号：CA2387283

申请日：2002-05-23

Applicant: HOFFMANN LA ROCHE

Inventor： BHULLAR RAGHBIR S ,  WILSEY CHRISTOPHER D ,  REISER WOLFGANG O L ,  AUSTERA JOHN T

IPC: G01N27/403 ,  G01N33/483 ,  C12M1/40 ,  C12Q1/00 ,  G01N27/327 ,  G01N27/416 ,  G01N33/48

Abstract: The present invention relates to a biosensor. The biosensor includes a suppo rt substrate having first and second ends, electrodes positioned on the support substrate , the electrodes cooperating with one another to define electrode arrays situated adjacent to the first end, a spacer substrate positioned on the support substrate, and a cover positioned on the spacer substrate. The cover cooperates with the support substrate to define a channel. The channel includes an inlet adjacent to the first end and opposite ends. Each electrod e array is positioned in the channel adjacent to one of the ends.

公开(公告)号：HK1094599A1

公开(公告)日：2007-04-04

申请号：HK07101708

申请日：2007-02-13

Applicant: HOFFMANN LA ROCHE

Inventor： WILSEY CHRISTOPHER D ,  MOSOIU DAN

IPC: B05C20060101 ,  C12Q20060101 ,  G01N20060101 ,  G01N27/26 ,  G01N31/22 ,  G01N33/00 ,  G01N33/487 ,  G01N33/558

Abstract: Methods of manufacturing test strips are disclosed. Embodiments include laminating a two piece covering layer over a substrate with a series of cavities, wherein the two pieces are separated by a gap, the gap forming a vent opening for the cavities. Other embodiments include covering the sample receiving chamber with a cover thinner than the cover covering the body of the test strip, the thicker body cover absorbing more force than the chamber cover to reduce the possibility of adhesives squeezing out from under the chamber cover. The present invention also provides an advantageous method of mass-producing the inventive test strips without having to align the slot or vent opening laterally with respect to the test strips and without having to punch a vent opening. The method is also well suited to mass production by roll processing techniques.

公开(公告)号：CA2530297A1

公开(公告)日：2004-12-29

申请号：CA2530297

申请日：2004-06-18

Applicant: HOFFMANN LA ROCHE

Inventor： BURKE DAVID W ,  MARQUANT MICHAEL ,  WILSEY CHRISTOPHER D ,  FRITZ MICHAEL ,  MOSOIU DAN ,  ZAPF UDO

IPC: G01N27/30 ,  C12Q1/00 ,  G01N27/26 ,  G01N31/22 ,  G01N33/00 ,  G01N33/487 ,  G01N33/558

Abstract: A test strip (10) having a small sample-receiving chamber on the order of le ss than 1 microliter includes a reagent layer (33) that extends across the widt h of the test strip and also extends to the sample-receiving end (36), such th at the edges of the reagent layer are aligned with the side and dose receiving edges of the test strip. The hydrophilic reagent extending to the dosing edg e of the strip promotes wicking of the sample into the test strip. The end and side edges of the reagent layer are preferably formed as part of a cutting process that forms individual test strips from a larger web, which results i n a smooth and thin reagent layer with a uniform thickness, substantially covering the entire floor of the sample-receiving chamber. The inventive mas s production process helps improve the reproducibility of the quantity, location, thickness and other properties of the reagent layer, which in turn improves the accuracy of the test result.

公开(公告)号：AU2002360400A8

公开(公告)日：2003-06-10

申请号：AU2002360400

申请日：2002-11-18

Applicant: HOFFMANN LA ROCHE ,  ROCHE DIAGNOSTIC CORP

Inventor： WILSEY CHRISTOPHER D ,  SURRIDGE NIGEL A

IPC: G01N27/327 ,  C12Q1/00 ,  G01N27/00 ,  G01N27/30 ,  G01N27/403 ,  G01N27/416 ,  G01N33/487

Abstract: The present invention concerns an array of micro- electrodes is disposed on to a flexible substrate.. Typically the array comprises electrode elements having a width greater than 15 µm, preferably from 15 µm to 100 µm. Such a micro-arrays may be employed in electrochemical biosensors which can be used to determine the concentration of a medically significant analyte such a glucose in sample materials like blood.

公开(公告)号：CA2387283A1

公开(公告)日：2002-11-25

申请号：CA2387283

申请日：2002-05-23

Applicant: HOFFMANN LA ROCHE

Inventor： WILSEY CHRISTOPHER D ,  BHULLAR RAGHBIR S ,  AUSTERA JOHN T ,  REISER WOLFGANG O L

IPC: G01N33/483 ,  C12Q1/00 ,  G01N27/327 ,  G01N27/416 ,  G01N27/28 ,  G01N33/48

Abstract: The present invention relates to a biosensor. The biosensor includes a suppo rt substrate having first and second ends, electrodes positioned on the support substrate , the electrodes cooperating with one another to define electrode arrays situated adjacent to the first end, a spacer substrate positioned on the support substrate, and a cover positioned on the spacer substrate. The cover cooperates with the support substrate to define a channel. The channel includes an inlet adjacent to the first end and opposite ends. Each electrod e array is positioned in the channel adjacent to one of the ends.

公开(公告)号：NZ745469A

公开(公告)日：2019-10-25

申请号：NZ74546913

申请日：2013-10-31

Applicant: HOFFMANN LA ROCHE

Inventor： WILSEY CHRISTOPHER D

IPC: C12Q1/00 ,  C12Q1/32 ,  C12Q1/54

Abstract: Reagent materials and associated test elements are provided. In particular described is a reagent material comprising a hydroxybutyrate dehydrogenase and a coenzyme selected from the group consisting of thio-NAD, thio-NADP, and a compound according to formula (I), test elements comprising the reagent material and methods of using the test element. The reagent material allows for rapid assessment of ketone levels. Also provided is a test element having dual functionality includes a first coenzyme-dependent enzyme or a substrate for the first enzyme, a second coenzyme-dependent enzyme or a substrate for the second enzyme, and a coenzyme selected from the group consisting of thio-NAD, thio-NADP, and a compound according to formula (I).

公开(公告)号：ZA201502999B

公开(公告)日：2016-09-28

申请号：ZA201502999

申请日：2015-04-30

Applicant: HOFFMANN LA ROCHE

Inventor： WILSEY CHRISTOPHER D

Abstract: Reagent materials and associated test elements are provided. In one embodiment, a test element having dual functionality includes a first coenzyme-dependent enzyme or a substrate for the first enzyme, a second coenzyme-dependent enzyme or a substrate for the second enzyme, and a coenzyme selected from the group consisting of thio-NAD, thio-NADP, and a compound according to formula (I). In one aspect, the first analyte is hydroxybutyrate and the first enzyme is a hydroxybutyrate dehydrogenase, and the second analyte is glucose and the second enzyme is a glucose dehydrogenase or a glucose oxidase. Other aspects of the subject application are directed to unique reagent materials. Further embodiments, forms, objects, features, advantages, aspects, and benefits shall become apparent from the description and drawings.

公开(公告)号：CA2831068C

公开(公告)日：2016-06-07

申请号：CA2831068

申请日：2012-04-12

Applicant: HOFFMANN LA ROCHE

Inventor： BUCILLI SERGIO ,  JOSEPH ABNER D ,  WILSEY CHRISTOPHER D

IPC: B05D1/26 ,  B05C5/02 ,  B05C11/10

Abstract: Systems, apparatuses, techniques and processes for applying a wet film (18) to a substrate (16) using a slot die (22) are provided. In one form, the air pressure around at least a portion of the discharge end of the slot die is adjustable by the application of a vacuum force (23) in order to control the width and thickness of the wet film being applied to the substrate. In one aspect of this form, the wet film is a narrow, continuous stripe of reagent material applied to a moving web of the substrate from which a plurality of test elements will be obtained. However, different forms and applications are also envisioned.

公开(公告)号：CA2644157C

公开(公告)日：2016-05-24

申请号：CA2644157

申请日：2002-11-18

Applicant: HOFFMANN LA ROCHE

Inventor： WILSEY CHRISTOPHER D

IPC: G01N27/30 ,  G01N27/327 ,  C12Q1/00 ,  C40B30/08 ,  C40B60/04 ,  G01N27/00 ,  G01N27/403 ,  G01N27/416 ,  G01N33/483 ,  G01N33/487 ,  G01N33/49 ,  G01N33/66

Abstract: A micro-array of electrodes is disposed proximal to a flexible substrate, such a micro-arraymay be employed in electronic components and sensors, typically the array comprises electrode elements having a width greater than 15 µm , especially greater than 15 µm to 100 µm, the micro-array may be employed in a method of detecting an analyte in medical diagnosis and other fields.

公开(公告)号：CA2719255C

公开(公告)日：2016-05-03

申请号：CA2719255

申请日：2009-03-25

Applicant: HOFFMANN LA ROCHE

Inventor： WILSEY CHRISTOPHER D ,  GHOSHAL MITALI ,  WIEDER HERBERT

IPC: C12Q1/00 ,  C12Q1/32 ,  G01N33/66

Abstract: A chemistry matrix for use in determining the concentration of an analyte in a biological fluid includes a glucose dehydrogenase, nicotinamide adenine dinucleotide, an alkylphenazine quaternary salt, and a nitrosoaniline. The chemistry matrix is used with an electrochemical biosensor to determine the concentration of an analyte after a reaction occurs within the biosensor, at which time an analysis is completed to determine the concentration. A method of determining the concentration of an analyte using the chemistry matrix of glucose dehydrogenase, nicotinamide adenine dinucleotide, an alkylphenazine quaternary salt, and a nitrosoaniline is another aspect that is described. The method also further features test times of five seconds or less. Methods utilizing the new chemistry matrix can readily determine an analyte such as blood glucose at concentrations of from about 20-600 mg/dL at a pH of from about 6.5 to about 8.5.