公开(公告)号：ES2305912T3

公开(公告)日：2008-11-01

申请号：ES05000414

申请日：1995-10-27

Applicant: IDEXX LAB INC

Inventor： EHRENFELD ELIZABETH ,  FRICKER COLIN ,  TOWNSEND DAVID E

IPC: C12Q1/10 ,  C12Q1/20 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/54

Abstract: Un método para detectar la presencia o ausencia de un microbio objetivo en una muestra líquida biológica o medioambiental que comprende la etapa de equilibración térmica de dicha muestra líquida con un medio de detección específico para un microbio objetivo que tiene un indicador de nutriente que puede alterar una característica detectable de la muestra si dicho microbio objetivo está presente en dicha muestra hasta una temperatura de incubación en un baño de agua antes de colocar dicha muestra líquida en un incubador seco y monitorizar la muestra para determinar si está alterada dicha característica detectable.

公开(公告)号：DK1403378T3

公开(公告)日：2008-06-23

申请号：DK03023038

申请日：1995-10-27

Applicant: IDEXX LAB INC

Inventor： FRICKER COLIN ,  EHRENFELD ELIZABETH ,  TOWNSEND DAVID E

IPC: C12Q1/10 ,  C12Q1/20 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/54

公开(公告)号：AT387504T

公开(公告)日：2008-03-15

申请号：AT03023038

申请日：1995-10-27

Applicant: IDEXX LAB INC

Inventor： EHRENFELD ELIZABETH ,  FRICKER COLIN ,  TOWNSEND DAVID E

IPC: C12Q1/10 ,  C12Q1/20 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/54

公开(公告)号：BR9813307A

公开(公告)日：2000-08-22

申请号：BR9813307

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI ,  NAQUI ALI

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543 ,  C12Q1/00

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：BR9813287A

公开(公告)日：2000-08-22

申请号：BR9813287

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  TOWNSEND DAVID E ,  GU HAOYI ,  NAQUI ALI ,  GELARDI PAUL J

IPC: C12M1/34 ,  B01J19/00 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/04 ,  G01N33/18 ,  G01N33/48 ,  G01N33/53 ,  G01N33/543

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyte, or microorganism is determined.

公开(公告)号：CA2311059A1

公开(公告)日：1999-05-06

申请号：CA2311059

申请日：1998-10-27

Applicant: IDEXX LAB INC

Inventor： PIERSON MARK W ,  NAQUI ALI ,  GU HAOYI ,  TOWNSEND DAVID E

IPC: C12M1/22 ,  C12Q1/06

Abstract: Method and assay devices for the detection of the presence or amount of biological material, analyte(s), or microorganism(s) in a sample. The method includes the steps of liquefying the sample (if necessary) and distributing the liquefied sample over the surface of the assay device. The device may comprise an incubation plate, a dip stick device, or other devices. The devices have at least one reagent provided within the devices. Some devices have a generally flat horizontal surface which is divided into a plurality of recessed wells. Others have one or more surfaces with reagent island(s) immobilized thereon. Each well or reagent island is adapted to hold an aliquot of liquid. The wells or reagent islands are sized and shaped, and formed of a suitable material, to hold the aliquot within the well or reagent island by surface tension. Any excess liquid from the liquefied sample is drained from the surface of the device. The method then involves incubating the assay device until the presence or amount of the biological material, analyze, or microorganism is determined.

公开(公告)号：CA2237639A1

公开(公告)日：1997-05-22

申请号：CA2237639

申请日：1996-11-12

Applicant: IDEXX LAB INC

Inventor： NAQUI ALI ,  PIERSON MARK W ,  CROTEAU ANDREW J ,  TOWNSEND DAVID E

IPC: G01N33/48 ,  B01L3/00 ,  C12M1/20 ,  C12M1/34 ,  G01N1/00 ,  C12Q1/06 ,  C12M1/22

Abstract: Method for detection of a biological material in a sample. The method includes the steps of liquefying the sample (if necessary) and pouring the liquefied sample into the incubation plate. The incubation plate has a generally flat horizontal surface and the surface is divided into a plurality of at least 20 recessed wells. Each well is adapted to hold an aliquot of liquid and is sized and shaped, and formed of a suitable material, to hold the aliquot within the well by surface tension. Any excess liquid from the liquefied sample is poured from the surface of the plate. The method then involves incubating that incubation plate until the presence or absence of the biological material is determined.

公开(公告)号：CA2223451C

公开(公告)日：2009-01-06

申请号：CA2223451

申请日：1996-05-31

Applicant: IDEXX LAB INC

Inventor： CHEN CHUN-MING ,  TOWNSEND DAVID E

IPC: C12Q1/34 ,  G01N33/02 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/37 ,  C12Q1/40 ,  C12Q1/42 ,  C12Q1/44

Abstract: This invention relates to a method for detecting the existence or measuring the concentration of total viable bacteria in a test sample from a food product. A medium is provided which contains three or more different enzyme substrates each having a nutrient moiety and a detectable moiety linked together. When a substrate is hydrolysed by a bacterial enzyme to create a separate detectable moiety, it causes or produces a detectable signal. These substrates produce detectable signals when any one of a phosphatase enzyme, a glycosidase enzyme or a peptidase enzyme is present in the medium.

公开(公告)号：DK1528106T3

公开(公告)日：2008-08-18

申请号：DK05000414

申请日：1995-10-27

Applicant: IDEXX LAB INC

Inventor： EHRENFELD ELIZABETH ,  FRICKER COLIN ,  TOWNSEND DAVID E

IPC: C12Q1/10 ,  C12Q1/20 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/54

Abstract: The invention features a medium for the detection of target microbes in a liquified environmental or biological sample in less than 18 hours.

公开(公告)号：ES2298457T3

公开(公告)日：2008-05-16

申请号：ES03023038

申请日：1995-10-27

Applicant: IDEXX LAB INC

Inventor： EHRENFELD ELIZABETH ,  FRICKER COLIN ,  TOWNSEND DAVID E

IPC: C12Q1/10 ,  C12Q1/20 ,  C12N1/20 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/54

Abstract: Medio específico para coliformes no gelificado, líquido que permite la detección de la presencia o ausencia de microbios objetivo en una muestra medioambiental o biológica licuada que comprende: vitaminas, una fuente de iones amonio, un tampón, un agente antifúngico, un indicador de nutriente, un antibiótico que evita o inhibe el crecimiento y la reproducción de una o más especies o grupos de microbios que no son objetivo, un inductor de una enzima que cataliza el metabolismo del indicador de nutriente, un precursor de ADN, ácido D-glucónico 0, 13 - 0, 16 g/l, una fuente de sulfito, aminoácidos en una concentración de al menos: alanina 0, 025 g/l, arginina 0, 030 g/l, ácido aspártico 0, 056 g/l, ácido glutámico 0, 1 g/l, glicina 0, 015 g/l, prolina 0, 09 g/l, cistina 0, 002 g/l, histidina 0, 006 g/l, isoleucina 0, 01 g/l, leucina 0, 030 g/l, lisina 0, 03 g/l, metionina 0, 01 g/l, fenilalanina 0, 018 g/l, serina 0, 029 g/l, treonina 0, 018 g/l, triptófano 0, 003 g/l, tirosina 0, 0064 g/l y valina 0, 023 g/l, hierro, calcio, fosfato, potasio, sodio, cobalto, cobre, plomo, magnesio, manganeso, cloruro, estaño y/o zinc y piruvato de sodio, que puede actuar para permitir la viabilidad y reproducción de los microbios objetivo a una tasa tal que puede observarse cualquier cambio detectable en dicha muestra en 18 horas para una muestra con menos de 10 microbios objetivo por 100 ml.