公开(公告)号：KR1020090035302A

公开(公告)日：2009-04-09

申请号：KR1020070100513

申请日：2007-10-05

Applicant: 대한민국(농촌진흥청장) ,  경상대학교산학협력단

Inventor： 윤두학 ,  전진태 ,  문희주 ,  임현태

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2537/143

Abstract: A method for discrimination between Korean beef cattle and imported cattle using a multiplexing PCR and a primer therefore are provided to distinguish species of cattle by analyzing gene loci rapidly and accurately. A method for discrimination between Korean beef cattle and imported cattle using a multiplexing PCR comprises the following steps of: obtaining DNA of cattle having two pairs of primers and a plurality of gene loci; amplifying a base sequence comprising each locus of a DNA sample into a multiplexing PCR; and measuring characteristic form and quality of an allele of each microsatellite locus.

Abstract translation: 提供了使用复用PCR和引物来区分韩国肉牛和进口牛的方法，以通过快速且准确地分析基因位点来区分牛的种类。 使用复用PCR鉴别韩国肉牛和进口牛的方法包括以下步骤：获得具有两对引物和多个基因座的牛的DNA; 将包含DNA样品的每个位点的碱基序列扩增到多重PCR中; 并测量每个微卫星位点等位基因的特征形式和质量。

公开(公告)号：KR1020080033825A

公开(公告)日：2008-04-17

申请号：KR1020060100050

申请日：2006-10-14

Applicant: 경상대학교산학협력단

Inventor： 전진태 ,  김재환 ,  임현태 ,  서보영 ,  이상호 ,  이재봉

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6844 ,  C12Q1/6827 ,  C12Q2537/143 ,  C12Q2563/107 ,  C12Q2565/125 ,  G01N33/5308

Abstract: A process for genotyping the cSNPs(complementary single nucleotide polymorphism) in CFB(complement factor B) gene localized to the SLA(swine leukocyte antigen) class III is provided to improve rapidness and economical efficiency of the genotyping as compared with conventional PCR-RFLP (polymerase chain reaction- restriction flagment-lengh polymorphism) by simultaneously analyzing cSNPs with multiplex PCR. A process for simultaneously genotyping three cSNPs in CFB gene localized to the SLA class III comprises the steps of: (1) preparing 3 fluorescence-labeled primer sets containing primers having the nucleotide sequences of SEQ ID NO:1 to SEQ ID NO:9 by using ARMS(amplification refractory mutation system); (2) performing multiplex PCR of a DNA sample of pig by using the primer sets; and (3) subjecting the PCR products to electrophoresis and analyzing the genotypes.

Abstract translation: 提供了定位于SLA（猪白细胞抗原）III类的CFB（补体因子B）基因中的cSNPs（互补单核苷酸多态性）进行基因分型的过程，以提高基因分型的快速性和经济效率，与常规PCR-RFLP 聚合酶链反应 - 限制性标记 - 长度多态性）通过多重PCR同时分析cSNPs。 同时对位于SLA III类的CFB基因中的三个cSNP进行基因分型的方法包括以下步骤：（1）制备含有具有SEQ ID NO：1至SEQ ID NO：9的核苷酸序列的引物的3个荧光标记引物组， 使用ARMS（扩增难治性突变系统）; （2）使用引物组进行猪的DNA样品的多重PCR; 和（3）对PCR产物进行电泳和分析基因型。