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41.发明公开
公开(公告)号：KR1020140064567A
公开(公告)日：2014-05-28
申请号：KR1020120132012
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG21 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG21 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG21 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG21 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及一种来源于黑山羊瘤胃微生物的纤维蛋白原酶cel-KG21基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因的cel-KG21基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG21基因，通过提供纤维蛋白原酶-GC21基因和蛋白质产物，可以将其用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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42.发明公开
公开(公告)号：KR1020140064564A
公开(公告)日：2014-05-28
申请号：KR1020120132007
申请日：2012-11-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a fibrinogenase cel-KG44 gene derived from rumen microorganisms of a black goat and uses thereof, and more specifically, to a cel-KG44 gene which is a novel fibrinogenase gene selected from rumen microorganisms of a black goat and a method of providing protein products thereof and manufacturing feed additives, a detergent composition, and biofuel by using the same. According to the present invention, a cel-KG44 gene which is a novel fibrinogenase gene derived from the rumen microorganisms of a black goat can be selected, and by providing the fibrinogenase cel-KG44 gene and protein products therethrough, they can be used for developing low-priced feed additives, softener, and detergent and actively used for production of biofuel by decomposing fibrins which are rich sources.
Abstract translation: 本发明涉及来自黑山羊瘤胃微生物的纤维蛋白原酶cel-KG44基因及其用途，更具体地说，涉及一种选自黑山羊瘤胃微生物的新型纤维蛋白原酶基因cel-KG44基因 提供其蛋白质产物和制造饲料添加剂，洗涤剂组合物和使用它们的生物燃料。 根据本发明，可以选择作为来自黑山羊的瘤胃微生物的新型纤维蛋白原酶基因的cel-KG44基因，通过提供纤维蛋白原酶cel-KG44基因和蛋白质产物，可以用于显影 低价饲料添加剂，软化剂和洗涤剂，并通过分解富集的纤维素积极地用于生产生物燃料。
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公开(公告)号：KR101376277B1
公开(公告)日：2014-03-20
申请号：KR1020120023999
申请日：2012-03-08
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은한우근내지방도연관분자표지및 이를이용한한우육질진단방법에관한것으로서, 보다상세하게는 FAS 유전자의 SNP를이용한한우근내지방도연관분자표지및 이를이용한한우육질진단방법에관한것이다. 한우 FAS 유전자의단일염기다형을이용하여근육내 지방축적에대한분자생물학적수준에서의해석이가능하며, 이를바탕으로고급육생산한우의조기진단및 선발과유용유전자원의산업적활용이가능하다. 즉, FAS 유전자의특정유전자형을보유하고있는개체를조기에진단하고선발육종하여한우육종개량사업의효율성과개량성과를극대화하고동시에우리나라고유의한우유전자원을보다우수하게개량하고보존하는효과를얻을수 있다.
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公开(公告)号：KR1020130068010A
公开(公告)日：2013-06-25
申请号：KR1020110135116
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel-KG52, cellulase, is provided to be used as a feed additive, to produce a detergent and biofuel, and to be used in industrial fields. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell, and overexpressing cellulase.
Abstract translation: 目的：提供cel-KG52，纤维素酶的产品，用作饲料添加剂，生产洗涤剂和生物燃料，并用于工业领域。 构成：来自黑山羊的反刍动物胃微生物的纤维素酶蛋白具有序列号2的氨基酸序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞和过表达纤维素酶的步骤。
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公开(公告)号：KR1020130068009A
公开(公告)日：2013-06-25
申请号：KR1020110135115
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel8-KG47 gene, endoglucanase, which is acquired by searching cellulose decomposition enzyme from a ruminant stomach microorganism of a black goat is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase from the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing endoglucanase.
Abstract translation: 目的：提供一种通过从黑山羊的反刍动物胃微生物中搜索纤维素分解酶获得的cel8-KG47基因，内切葡聚糖酶的产物，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料 。 构成：来自黑山羊的反刍动物胃微生物的内切葡聚糖酶蛋白具有序列号2的氨基酸序列。基因编码内切葡聚糖酶蛋白质。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切葡聚糖酶的方法包括：将重组载体转导至宿主细胞的步骤; 以及过表达内切葡聚糖酶的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020130068008A
公开(公告)日：2013-06-25
申请号：KR1020110135114
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/248 , A23K10/14 , C11D3/38636 , C12N15/67 , C12P7/649
Abstract: PURPOSE: A product of cel10-KG42 gene, endoxylanase, which is obtained by searching cellulose decomposing enzymes from a ruminant stomach microorganism of a black goat is provided to be used in a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoxylanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. The gene encodes endoxylanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoxylanase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing endoxylanse.
Abstract translation: 目的：提供一种通过从黑山羊的反刍动物胃微生物中搜索纤维素分解酶而获得的cel10-KG42基因的内切木聚糖酶，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料 。 构成：来自黑山羊的反刍动物胃微生物的内切木聚糖酶蛋白具有序列号2的氨基酸序列。该基因编码内切木聚糖酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切木聚糖酶的方法包括将重组载体转导至宿主细胞并过量表达内生木聚糖的步骤。
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公开(公告)号：KR1020130068002A
公开(公告)日：2013-06-25
申请号：KR1020110135108
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel-KG20, cellulase, is provided to be used as a feed additive, to produce a detergent and a biofuel, and to be industrially used. CONSTITUTION: A cellulase protein derived from a ruminant stomach microorganism of a black goat contains a microorganism sequence of sequence number 2. A gene encodes the cellulase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of cellulase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing cellulase.
Abstract translation: 目的：提供cel-KG20，纤维素酶的产品，用作饲料添加剂，生产洗涤剂和生物燃料，并在工业上使用。 构成：衍生自黑山羊的反刍动物胃微生物的纤维素酶蛋白含有序列号2的微生物序列。基因编码纤维素酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量纤维素酶的方法包括将重组载体转导到宿主细胞并过度表达纤维素酶的步骤。
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公开(公告)号：KR101236298B1
公开(公告)日：2013-02-26
申请号：KR1020100059237
申请日：2010-06-22
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 새로운 돼지 등지방두께 진단용 바이오마커 (biomarker) 및 이의 선별방법에 관한 것으로, 서열번호 1 내지 서열번호 40의 염기 서열 중 하나 이상을 포함하는 돼지 등지방두께를 판정하기 위한 본 발명의 단일염기 다형성 바이오마커를 이용할 경우 표현형평가에 의한 선발효과보다 25 내지 35% 더 정확한 유전능력 측정이 가능하여 종돈의 유전능력 개량량을 획기적으로 늘릴 수 있으며, 돼지 등지방두께는 현재 도체 등급을 평가하는 중요한 요인 중의 하나이므로 우수 종돈의 국내 육성에 크게 기여할 수 있다. 따라서 종축 개량에 있어서 세계적인 추세에 따라 본 연구에서 발견된 등지방두께 관련 단일염기다형성 유전자형은 앞으로 돼지 선발에 유전적 DNA 마커로서 매우 효과적이다.
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公开(公告)号：KR101224007B1
公开(公告)日：2013-01-24
申请号：KR1020100059236
申请日：2010-06-22
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 새로운 돼지 육질 진단용 바이오마커 (biomarker)들, 그들의 제조방법 및 그들의 용도에 관한 것이다. 보다 상세하게는, 본 발명은 돼지 육질을 판정할 수 있는 단일염기 다형성 (SNP) 바이오마커들, (1) 돼지 육질과 연관된 단일염기 다형성 (SNPs)을 결정하고; (2) 양적 형질 유전좌위 (QTL)와의 연관성을 검정하며; 및 (3) 이 결과를 기초로 하여 돼지 삼겹살 함량의 형질을 분석하는; 과정으로 이루어진 상기 바이오마커들의 선발 방법 및 상기 바이오마커들을 종돈 선발 등에 사용하는 용도에 관한 것이다. 본 발명의 돼지 육질 진단용 바이오마커는 종돈의 유전 능력을 보다 정확하게 측정할 수 있어 우수 종돈의 육성에 널리 사용될 수 있다.
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公开(公告)号：KR101156047B1
公开(公告)日：2012-06-27
申请号：KR1020090088414
申请日：2009-09-18
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A microsatellite marker and a method for identifying dog individual using the same are provided to perform multiplex PCR and to perform parental testing. CONSTITUTION: A microsatellite marker which is able to distinguish dog individual is a combination of one or more sets among: a first set having FH3005, FH2537, FH3921, FH3381, FH3116, FH3372, REN62M06, REN277O05, REN51C16, FH3399, and FH3027; a second set having FH2097, REN204K13, FH1014, FH3058, FH2834, FH2712, REN112C08, FH2998, FH2584, and REN197E16; and a third set having FH2054, REN181K04, FH2079, REN01O23, FH2582, and FH2790. A method for identifying dog individual comprises: a step of performing multiple PCR using microsatellite marker; and a step of detecting allele through electrophoresis and determining genotype.
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