Abstract:
PURPOSE: A PNA microarray for identifying and diagnosing aerobic gram-positive food poisoning bacteria is provided to accurately and quickly diagnose Staphylococcus aureus and Listeria monocytogenes from other bacteria. CONSTITUTION: A PNA microarray for identifying and diagnosing aerobic gram-positive food poisoning bacteria contains a PNA(peptide nucleic acid) probe of sequence numbers 1 and 2 and a primer of sequence numbers 3-6. The aerobic gram-positive food poisoning bacteria include Staphylococcus aureus or Listeria monocytogenes.
Abstract:
본 발명은 소의 PrP 펩타이드 중 특정부위를 선발하고 이를 유전자 적중 마우스에 접종하여 면역시킨 후 여기에서 분리한 면역 B 세포와 마우스 골수종유래세포를 융합하여 제조한 융합세포주; 상기 융합세포주로부터 생산되고 소해면상뇌증의 변형프리온(PrP sc )을 소의 정상프리온 및 사슴만성소모성질병 및 스크래피(Scrapie) 등의 다른 전염성해면상뇌증의 변형프리온과 감별하여 검출해 내는 단클론항체 및 그 용도에 관한 것이다. 소해면상뇌증, 변형프리온, 단클론항체, 융합세포주, PrP 펩타이드, 전염성해면상뇌증
Abstract:
PURPOSE: A method for diagnosing vesicular stomatitis virus serotype New Jersey is provided to detect New Jersey specific antibody in the livestock serum. CONSTITUTION: A gene recombinant baculovirus expression vector contains a gene of sequence number 1 vesicular stromatitis virus serotype New Jersey protein. A method for preparing the glycoprotein comprises: a step of co-infecting a vector and baculovirus gene to a host cell; a step of culturing the infected host cell and separating supernatant containing the recombinant baculovirus from the culture; a step of infecting the host cell with the supernatant; a step of collecting cell pellet and crushing the cells; and a step of isolating the glycoprotein from the crushed cell culture. The baculovirus gene is pBacPAK6. The host cell is an insect cell.