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公开(公告)号：KR101018629B1
公开(公告)日：2011-03-07
申请号：KR1020090003072
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68 , C12N15/10
Abstract: 본 발병은 벚나무 속 식물에 발생하는 자두위축바이러스(PDV)를 진단하기 위한 PCR 검사시스템에 관한 것이다. PCR 검사시스템은 PDV 진단용 최적 프라이머 조합 및 이에 부합하는 검정용 프라이머 조합(nested primer)과 이를 뒷받침할 수 있는 종 특이 프라이머 조합 및 양성대조구를 포함하고 있다.
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42.发明公开벼 줄무늬잎마름병 바이러스 보독충 진단용 양성대조군의 제조방법, 양성대조시약 및 이를 이용한 진단방법 有权用于诊断RSV VIRULIFUSUS INSECT，积极控制剂和使用其的诊断方法的积极控制的制备方法
公开(公告)号：KR1020100085384A
公开(公告)日：2010-07-29
申请号：KR1020090004637
申请日：2009-01-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/70 , G01N33/569 , C12Q1/68
CPC classification number: C12Q1/70 , C12Q1/6844 , G01N33/56983
Abstract: PURPOSE: A method for manufacturing a positive control group for diagnosing RSV viruliferous insect is provided to enhance diagnosis reliability. CONSTITUTION: A method for manufacturing a positive control group for diagnosing RSV viruliferous insect comprises: a step of inoculating Laodelphax striatella to a RSV viruliferous infected plant; a step of mixing and pulverizing Laodelphax striatella; a step of searching viruliferous insect rate and pulverizing Laodelphax striatella when the viruliferous insect rate is more than 30%; and a step of pulverizing at least 10 viruliferous insects. A diagnosis method is performed by ELISA(Enzyme-linked immunosorbent assay) or RT-PCR(reverse transcriptase polymerase chain reaction).
Abstract translation: 目的：提供一种用于诊断RSV毒性昆虫的阳性对照组的方法，以提高诊断的可靠性。 构成：用于制造用于诊断RSV病毒性昆虫的阳性对照组的方法，包括：将羊角纹鳞状芽孢杆菌接种至感染RSV的病毒植物的步骤; 混合粉碎黑麦草的步骤; 当恶臭昆虫率超过30％时，探索恶臭昆虫率并粉碎黑麦草纹状体的步骤; 以及粉碎至少10种毒力昆虫的步骤。 通过ELISA（酶联免疫吸附测定）或RT-PCR（逆转录酶聚合酶链反应）进行诊断方法。
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公开(公告)号：KR1020100083608A
公开(公告)日：2010-07-22
申请号：KR1020090003074
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68 , C12N15/10
CPC classification number: C12Q1/70 , C12N15/11 , C12Q1/686 , G01N33/56983
Abstract: PURPOSE: A PCR test system for diagnosing melon nectrotic spot virus(MNSV) is provided to improve diagnosis detection and accuracy. CONSTITUTION: A primer for diagnosing melon nectrotic spot virus is a primer combination of sequence numbers 3 and 13, sequence number 4 and 11, sequence numbers 5 and 18, sequence number 6 and 18, sequence numbers 6 and 19, or sequence numbers 7 and 17.
Abstract translation: 目的：提供一种用于诊断瓜类nectrotic斑病毒（MNSV）的PCR检测系统，以提高诊断检测和准确性。 构成：用于诊断瓜类nectrotic斑病毒的引物是序列号3和13，序列号4和11，序列号5和18，序列号6和18，序列号6和19或序列号7和序列号7的引物组合 17。
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公开(公告)号：KR1020100083607A
公开(公告)日：2010-07-22
申请号：KR1020090003073
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68
CPC classification number: C12Q1/70 , C12N15/11 , C12Q1/686 , G01N33/56983
Abstract: PURPOSE: A PCR test system for diagnosing GFLV(grapevine fanleaf virus) is provided to diagnose and to easily diagnose. CONSTITUTION: A primer for diagnosing grapevine fanleaf virus is primer complex sequence numbers 8 and 19, sequence numbers 9 and 18, sequence number 11 and 16, and sequence number 12 and 15. The diagnosis is performed by RT-PCR using the primer with a genome RNA as a template.
Abstract translation: 目的：提供诊断GFLV（葡萄叶扇叶病毒）的PCR检测系统进行诊断和诊断。 构成：用于诊断葡萄扇叶病毒的引物是引物复合序列号8和19，序列号9和18，序列号11和16以及序列号12和15.通过RT-PCR进行诊断，使用引物 基因组RNA作为模板。
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公开(公告)号：KR1020100083606A
公开(公告)日：2010-07-22
申请号：KR1020090003072
申请日：2009-01-14
Applicant: 대한민국(농촌진흥청장)
IPC: G01N33/569 , C12N15/33 , C12Q1/68 , C12N15/10
CPC classification number: C12Q1/70 , C12N15/11 , C12Q1/686 , G01N33/56983
Abstract: PURPOSE: A PCR test system for diagnosing PDV(prune dwarf virus) generated in Japanese flowering cherry is provided to accurately diagnose PDV infection. CONSTITUTION: A primer for diagnosing PDV(prune dwarf virus) is a primer combination of sequence numbers 11 and 23, sequence numbers 11 and 25, sequence numbers 11 and 26, sequence number 12 and 22, sequence numbers 12 and 23, sequence number 16 and 23, and sequence number 19 and 23. A method for diagnosing PDV comprises: a step of isolating genome RNA of virus sample; a step of performing RT-PCR using the primer with genome RNA as a template; and a step of analyzing RT-PCR product.
Abstract translation: 目的：提供用于诊断日本樱花樱桃产生的PDV（修剪矮病毒）的PCR检测系统，以准确诊断PDV感染。 构成：用于诊断PDV（修剪矮化病毒）的引物是序列号11和23，序列号11和25，序列号11和26，序列号12和22，序列号12和23，序列号16的引物组合 23和序列号19和23.诊断PDV的方法包括：分离病毒样品的基因组RNA的步骤; 使用具有基因组RNA作为模板的引物进行RT-PCR的步骤; 和分析RT-PCR产物的步骤。
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Patent Agency Ranking
公开(公告)号：KR1020100039936A
公开(公告)日：2010-04-19
申请号：KR1020080098927
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/6827 , C12Q2600/112
Abstract: PURPOSE: A primer set for diagnosing GLV(Garlic latent virus), LYSV(Leek yellow stripe virus), GCLV(Garlic common latent virus), OYDV(Onion yellow dwarf virus) and Allexivirus is provided to detect all garlic virus with high detection limit and quickly detect virus diseases in Allium crop. CONSTITUTION: A primer set for diagnosing GLV(Garlic latent virus), LYSV(Leek yellow stripe virus), GCLV(Garlic common latent virus), OYDV(Onion yellow dwarf virus) and Allexivirus comprises a primer set of sequence numbers 1 and 2, primer set of sequence numbers 3 and 4, and primer set of sequence numbers 5 and 6. A kit for diagnosing GLV, LYSV, GCLV, OYDV, Allexivirus contains the primer set and reagent for RT-PCR.
Abstract translation: 目的：提供用于诊断GLV（大蒜潜伏病毒），LYSV（韭黄色条纹病毒），GCLV（大蒜共同潜隐病毒），OYDV（洋葱黄矮病毒）和亚克隆病毒）的引物组，以检测所有具有高检出限的大蒜病毒 并快速检测葱属作物中的病毒病。 构成：用于诊断GLV（大蒜潜伏病毒），LYSV（韭黄条纹病毒），GCLV（大蒜共同潜隐病毒），OYDV（洋葱黄矮病毒）和亚克隆病毒）的引物组包括序列号1和2的引物组， 序列号3和4的引物组以及序列号5和6的引物组。用于诊断GLV，LYSV，GCLV，OYDV，亚型转录病毒的试剂盒含有用于RT-PCR的引物组和试剂。
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公开(公告)号：KR1020100039935A
公开(公告)日：2010-04-19
申请号：KR1020080098926
申请日：2008-10-09
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/701 , C12Q1/6827 , C12Q2600/112
Abstract: PURPOSE: A primer set for diagnosing Allexivirus genus and a method for diagnosing the same are provided to economically and simply diagnose Allexivirus genus. CONSTITUTION: A primer set for diagnosing Allexivirus genus comprises a primer set of sequence numbers 1, 2, and 3; primer set of sequence numbers 4 and 5; or primer set of sequence numbers 1 and 6. A primer set for diagnosing Allexivirus genus comprises a primer set of sequence numbers 1, 2, and 3; primer set of sequence numbers 1, 4, and 5; and primer set of sequence numbers 1 and 6. The Allexivirus genus contains Garlic virus X, Garlic virus A, Garlic virus B, Garlic virus C, Garlic virus D, Garlic virus E, Shallot virus X, Garlic mite-borne mosaic virus and Garlic miteborne filamentous virus. A kit for diagnosing Allexivirus genus contains a primer set and reagent for RT-PCR.
Abstract translation: 目的：提供用于诊断相关病毒属的引物组及其诊断方法，以经济和简单地诊断相关病毒属。 构成：用于诊断亚链病毒属的引物组包含序列号1,2和3的引物组; 序列号4和5的引物组; 或序列号1和6的引物组。用于诊断同种异体病毒属的引物组包含序列号1,2和3的引物组; 序列号1,4和5的引物组; 序列号1和6的引物组。亚链病毒属包含大蒜病毒X，大蒜病毒A，大蒜病毒B，大蒜病毒C，大蒜病毒D，大蒜病毒E，青葱病毒X，大蒜螨携带花叶病毒和大蒜 螨虫丝状病毒。 用于诊断亚克力病毒属的试剂盒含有用于RT-PCR的引物组和试剂。
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公开(公告)号：KR101658663B1
公开(公告)日：2016-09-23
申请号：KR1020110117660
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 3024 내지 3028 및서열번호 3057 내지 3060의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 SBMV (Southern bean mosaic virus) 진단또는검출용올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 SBMV 진단또는검출용유전자칩, SBMV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 SBMV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 SBMV 또는검출용키트에관한것이다.
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公开(公告)号：KR101650562B1
公开(公告)日：2016-08-24
申请号：KR1020110117791
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 3449 내지 3476 및서열번호 3513 내지 3520의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 TSWV (Tomato spotted wilt virus) 올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 TSWV 진단또는검출용유전자칩, TSWV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 TSWV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 TSWV는검출용키트에관한것이다.
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公开(公告)号：KR101650556B1
公开(公告)日：2016-08-24
申请号：KR1020110117760
申请日：2011-11-11
Applicant: 한국생명공학연구원 , 대한민국(농촌진흥청장)
Abstract: 본발명은서열번호 1025 내지 1034, 서열번호 1045 내지 1049 및서열번호 2988의올리고뉴클레오티드및 이의상보적올리고뉴클레오티드로구성되는군으로부터선택되는하나이상의올리고뉴클레오티드를포함하는 PSV (Peanut stunt virus) 올리고뉴클레오티드프로브세트, 상기올리고뉴클레오티드프로브세트를포함하는 PSV 진단또는검출용유전자칩, PSV에감염된것으로추정되는시료로부터 RNA를분리하는단계, 상기분리된 RNA를주형으로하여 cDNA를합성하는단계, 상기합성된 cDNA를상기올리고뉴클레오티드프로브세트와접촉시켜상기 cDNA와프로브세트를혼성화시키는단계및 상기프로브와 cDNA 사이의혼성화정도를검출하는단계를포함하는 PSV를진단또는검출하는방법및 상기올리고뉴클레오티드프로브세트및 혼성화용액을포함하는 PSV는검출용키트에관한것이다.
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