Abstract:
The present invention relates to an improved pig and a preparation method thereof, and more specifically, to a method for preparing a pig having improved quality comprising a step of fixing SNP traits of genes involved in traits of flesh, the thickness of back fat, and the color of black hair of the pig, and to a pig having improved quality prepared by the method. According to the preparation method, the present invention can prepare an improved pig having improved traits of flesh, thickness of back fat, and color of black hair of the pig, thereby providing a high-quality pig and improving income of stock farms.
Abstract:
본 발명은 돼지의 육질형질 수준을 판단할 수 있는 SNP를 포함하는 일배체형 마커, 상기 일배체형 마커를 검출 또는 증폭할 수 있는 제제를 포함하는 돼지의 육질형질 수준 판단용 조성물, 상기 조성물을 포함하는 돼지의 육질형질 수준 판단용 키트 또는 마이크로어레이 및 상기 일배체형 마커에 포함된 SNP의 다형성 부위를 결정하는 단계를 포함하는 돼지의 육질형질 수준 판단방법에 관한 것이다. 본 발명의 일배체형은 돼지의 육질형질 수준을 판단하는 특이적 유전자 마커로서, 육안으로 구별되지 않는 돼지의 육질형질 수준을 객관적으로 평가할 수 있는 수단으로 사용되므로, 돼지육의 유통질서 확립에 이바지할 수 있을 것이다.
Abstract:
The present invention relates to a haplotype marker comprising an SNP capable of discriminating the level of meat quality of a pig; to a composition or discriminating the level of meat quality of a pig containing a reagent capable of detecting or amplifying the haplotype marker; to a kit or microarray for discriminating the level of meat quality of a pig comprising the composition; and to a method for determining the level of meat quality of a pig comprising a step of determining a polymorphic site of the SNP included in the haplotype marker. The haplotype marker of the present invention is a specific gene marker which discriminates the level of meat quality of a pig, and is used as a means capable of objectively evaluating the level of meat quality of a pig, which is not distinguished by the naked eye, thereby contributing to the establishment of order in the circulation of pig meat.
Abstract:
A meat improvement method is provided, which improves quality of the meat and reduces aging period of meat and the energy consumption required for the meat cooling. A meat improvement method comprises: a step for first cooling meat for 3 ~ 5 hours at -1 ~ 4deg.C after slaughter; a step for second cooling the cooled meat for 3 ~ 5 hours at 10 ~ 14deg.C; and a step for third cooling the cooled meat for 14 ~ 18 hours at -1 ~ 4deg.C. The second cooling is formed to appropriately control the post metabolism speed and to improve the meat.
Abstract:
A PCR(polymerase chain reaction) primer for selection of breeding pigs is provided to select a breeding pig having A-FABP(adipocyte fatty acid binding protein) genotype capable of improving intramuscular fat in fed pigs and growth traits in Duroc breed, so that fed pigs having high intramuscular fat and rapid growth traits are produced. An FBp primer set for selection of breeding pigs consists of an FBp forward primer having the nucleotide sequence of 5'-CTGTTCCCCAAAGCCTCTGC-3' and an FBp reverse primer having the nucleotide sequence of 5'-AGCTTAGAAGCCGTAACCTT-3'. A method for selection of breeding pigs comprises the steps of: performing PCR of DNA extracted from the blood or hair root of pigs with the FBp primer set; storing the PCR products at 4 deg. C; digesting the PCR products with a restriction enzyme HinfI and leaving them in an incubator at 37 deg. C for 4 hours or more; and subjecting the digested reaction solution to 10-15% polyacrylamide gel electrophoresis and staining the developed gel with ethidium bromide solution to determine the genotype.
Abstract:
본 발명은 번식돈의 산자수와육성률을 결정하는 경제형질 중의 하나인 돼지의 유두수를 판단할 수 있는 SNP 마커, 상기 SNP 마커를 검출 또는 증폭할 수 있는 제제를 포함하는 돼지의 유두수 판단용 조성물, 상기 조성물을 포함하는 돼지의 유두수 판단용 키트 및 상기 SNP 마커의다형성 부위를 결정하는 단계를 포함하는 돼지의 유두수 판단방법에 관한 것이다. 본 발명의 신규 SNP 마커 및 이를 이용한 파이로 시퀀싱 (Pyro-sequencing) 분석 방법은 번식형질인 돼지의 유두수를 조기에 판단할 수 있는 수단으로 사용될 수 있으므로, 우수한 종돈을 확대 생산할 수 있는 시스템을 구축하여, 양돈 산업의 경쟁력을 높이는데 이바지할 수 있을 것이다.