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公开(公告)号：KR1020150056915A
公开(公告)日：2015-05-28
申请号：KR1020130139545
申请日：2013-11-18
Applicant: 대한민국(농촌진흥청장)
IPC: C12N15/85 , C12N15/86 , A01K67/033
CPC classification number: C12N15/85
Abstract: 본발명은서열번호 3으로표시되는누에형질전환용고발현유전자프로모터, 상기고발현유전자프로모터를포함하는누에형질전환용고발현벡터, 및상기고발현벡터로형질전환된누에형질전환체에관한것이다. 본발명에따른누에형질전환용프로모터및 이를포함하는고발현벡터는종래의 BmA3 프로모터보다견사선또는누에생체에서의고발현을유도할수 있어, 누에에서의이형단백질생산성향상과정제과정의편리성에의하여산업적으로매우유용하다.
Abstract translation: 本发明涉及用于转化如SEQ ID NO：3所示的蚕的高表达基因启动子，包含用于转化蚕的高表达载体和由其转化的转基因蚕。 根据本发明，高表达基因启动子和载体可以比常规BmA3启动子更有效地诱导蚕丝或丝腺的高表达，从而增加蚕的变异蛋白质的产生，并使工业上更容易的纯化过程 利用。
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42.发明授权
公开(公告)号：KR101359510B1
公开(公告)日：2014-02-12
申请号：KR1020110120127
申请日：2011-11-17
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 누에 혈림프 특이적 발현 유전자의 프로모터 및 이 유전자의 최대 프로모터 활성영역에 관한 것이다.
본 발명의 누에 혈림프 특이적 발현 프로모터는 누에로부터 분리하여 서열번호 1로 표현되는 것이 특징이다.
본 발명에 의해, 누에의 혈림프에 특이적으로 발현되어 형질전환 누에로부터 유용한 유전자 재조합 단백질을 생산하도록 한 누에 혈림프 특이적 발현 유전자의 프로모터 및 이 유전자의 최대 프로모터 활성영역이 제공된다.-
43.发明公开인간 ＧＭ－ＣＳＦ２ 재조합 단백질을 갖는 누에 형질전환체의 제조방법 및 이를 통해 생산된 누에 형질전환체, 형질전환누에 无效具有人造粒细胞巨噬细胞集落刺激因子的丝状体转化的制造方法2重组蛋白和丝变性由方法获得的丝状体
公开(公告)号：KR1020140012406A
公开(公告)日：2014-02-03
申请号：KR1020120079203
申请日：2012-07-20
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a method for producing a silkworm transformant having human GM-CSF2 recombinant protein, a silkworm transformant produced thereby, and a transformed silkworm. The method for producing a silkworm transformant having human GM-CSF2 recombinant protein according to the present invention comprises the steps of: introducing bFibH promoter and 3xP3 promoter into a transfer vector to prepare an expression vector for silkworm transformation; and micro-injecting the expression vector for silkworm transformation into a silkworm egg to prepare a transduced silkworm transformant. The present invention provides: a method for producing a silkworm transformant, capable of mass-producing human-derived GM-CSF2 recombinant protein useful to humans at low costs; a silkworm transformant produced thereby; and a transformed silkworm.
Abstract translation: 本发明涉及具有人GM-CSF2重组蛋白，由其制备的蚕转化体和转化的蚕的蚕转化体的制备方法。 本发明的具有人GM-CSF2重组蛋白的蚕转化体的制造方法包括将bFibH启动子和3xP3启动子引入转移载体中以制备蚕转化表达载体的步骤。 并将蚕转化的表达载体微注射到蚕卵中以制备转导的蚕转化体。 本发明提供：能够以低成本大规模生产对人类有用的人源GM-CSF2重组蛋白质的蚕转化体的制造方法。 由此产生的蚕转化体; 和转化的蚕。
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公开(公告)号：KR1020130141973A
公开(公告)日：2013-12-27
申请号：KR1020120065146
申请日：2012-06-18
Applicant: 대한민국(농촌진흥청장)
Abstract: The present invention relates to a food composition containing Dpp protein originated from silkworm as an active ingredient for the prevention and treatment of bone-related diseases. The present invention provides a food composition for the prevention and treatment of bone-related diseases containing Dpp protein originated from silkworm which contains the amino acid sequence with the sequence number 2 or a base sequence with the sequence number 2 for coding the amino acid sequence. The food composition for the prevention and treatment of bone-related diseases contains the Dpp protein originated from silkworm which is separated from silkworm and induces the production of bones by improving the differentiation of osteoblast necessary for the bone production.
Abstract translation: 本发明涉及含有源自蚕的Dpp蛋白作为预防和治疗骨相关疾病的有效成分的食品组合物。 本发明提供一种用于预防和治疗含有源自蚕的Dpp蛋白质的骨相关疾病的食物组合物，其含有序列号2的氨基酸序列或编码氨基酸序列的序列号为2的碱基序列。 用于预防和治疗骨相关疾病的食物组合物含有源自蚕的Dpp蛋白质，其通过改善骨产生所需的成骨细胞的分化来诱导骨骼的产生。
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45.发明授权히알루론산―도파민 컨쥬게이트로 표면이 개질된 하이드록시아파타이트 나노입자를 포함하는 실크피브로인 나노섬유 및 이를 이용한 지지체 有权包含用羟丙酸/多巴胺结合物修饰的羟基磷酸酯的纳米纤维素和使用其的SCAFFOLD
公开(公告)号：KR101313898B1
公开(公告)日：2013-09-30
申请号：KR1020120044954
申请日：2012-04-27
Applicant: 대한민국(농촌진흥청장) , 서울대학교산학협력단
CPC classification number: D01F4/02 , A61F2/2846 , A61L24/00 , A61L27/3604 , D01D5/0015 , D01D10/06 , D01F1/10
Abstract: PURPOSE: The complex nanofiber of hydroxyapatite and silk fibroin, a two dimensional structure and a three dimensional structure supporter using the same is provided to easily facilitate the uniformity and guarantee of reappearance, is able to electrospinning for a long time, and useful for cell formation, bond tissue regeneration and supporter as its mechanical property is satisfied. CONSTITUTION: The complex nanofiber uniformly disperses the hydroxyapatite nano particle which surface is reformed with the hyaluronic acid-dopamine conjugate inside the silk fibroin nanofiber. Conjugate of the hyaluronic acid-dopamine is the formation of amide bond by combining the carboxyl group of hyaluronic acid and amine group of dopamine. The hydroxyapatite which surface is reformed with the hyaluronic acid-dopamine conjugate is the combination of two hydroxyl radicals of dopamine to the surface of the hydroxyapatite. The diameter of the complex nanofiber is 100-1000 nm.
Abstract translation: 目的：提供复合纳米纤维的羟基磷灰石和丝素蛋白，二维结构和三维结构支持体，使其易于均匀，保证再现，能长时间静电纺丝，可用于细胞形成 ，粘合组织再生和支持体作为其机械性能得到满足。 构成：复合纳米纤维将丝氨蛋白纳米纤维内透明质酸 - 多巴胺缀合物表面的羟基磷灰石纳米粒子均匀分散。 透明质酸 - 多巴胺的结合物是通过将透明质酸的羧基和多巴胺的胺基组合而形成酰胺键。 表面用透明质酸 - 多巴胺缀合物重整的羟基磷灰石是多巴胺的两个羟基自由基与羟基磷灰石表面的组合。 复合纳米纤维的直径为100-1000nm。
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Patent Agency Ranking
公开(公告)号：KR1020130051602A
公开(公告)日：2013-05-21
申请号：KR1020110116838
申请日：2011-11-10
Applicant: 한림대학교 산학협력단 , 대한민국(농촌진흥청장) , 서울대학교산학협력단
CPC classification number: A61L27/60 , A61L27/22 , A61L2430/34 , D01D5/0007
Abstract: PURPOSE: A 3 dimensional silk nano fiber film and a manufacturing method thereof are provided to control biodegradation speed by having similar structure with human skin in size and thickness. CONSTITUTION: A manufacturing method of 3 dimensional silk nano filber film for skin substitute comprises a process for preparing nano fiber undiluted solution, salt adding process and applying voltage and diffusing prepared nano fiber undiluted solution at the same time, and salt removing process from the diffused nano fiber film. On the salt adding process with the electro-spinning, the supply quantity and particle size controlled salt is sprayed on the nano fiber film to form an air gap. The size of the air gap is 10-100 micrometer and the thickness is 1-5 millimeters.
Abstract translation: 目的：提供一种三维丝纳米纤维膜及其制造方法，以通过与人体皮肤的尺寸和厚度相似的结构来控制生物降解速度。 构成：用于皮肤代用品的三维丝纳米薄膜的制造方法包括同时制备纳米纤维未稀释溶液，加盐过程和施加电压并扩散制备的纳米纤维未稀释溶液的方法，以及从扩散的脱盐过程 纳米纤维膜。 在电纺丝的加盐过程中，将供给量和粒度控制盐喷洒在纳米纤维膜上形成气隙。 气隙的大小为10-100微米，厚度为1-5毫米。
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公开(公告)号：KR1020110127324A
公开(公告)日：2011-11-25
申请号：KR1020100046741
申请日：2010-05-19
Applicant: 한림대학교 산학협력단 , 인하대학교 산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: A61F2/02 , A61L27/227 , C08J3/205 , C08L89/00
Abstract: PURPOSE: An artificial dura mater and a manufacturing method thereof are provided to guarantee safety in operation and to do not cause inflammation in the operation. CONSTITUTION: Silk fibroin aqueous solution is cast in smooth and flat place and is dried. The crystallization induction of the dried silk fibroin is executed. A crystallization induction step puts the dried silk fibroin is executed into an enclosure container in which relative humidity is over 80% and executes annealing with water.
Abstract translation: 目的：提供人造硬膜及其制造方法，以保证手术中的安全性，并且不会在手术中引起炎症。 构成：将丝素蛋白水溶液浇铸在光滑平坦的地方，并干燥。 干燥的丝素蛋白的结晶诱导被执行。 结晶诱导步骤将干燥的丝素蛋白进行到相对湿度超过80％的封闭容器中，并用水进行退火。
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48.发明公开누에 프로테인 디설피드 이소머레이즈 유전자를 이용한 재조합 인간 각질세포 성장인자의 제조방법 无效通过使用BOMBYX MORI蛋白质半胱氨酸异构酶基因生产重组人KERATINOCYTE生长因子的方法
公开(公告)号：KR1020110120662A
公开(公告)日：2011-11-04
申请号：KR1020100040172
申请日：2010-04-29
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for preparing recombinant human keratinocyte growth factor using Bombyx mori protein disulfide isomerase gene is provided to improve productivity. CONSTITUTION: A method for preparing recombinant human keratinocyte growth factor comprises: a step of transforming insect cell line with a recombinant expression vector containing the gene; a step of simultaneously or gradually transforming insect cell line with a recombinant vector containing human keratinocyte growth factor gene; a step of selecting transformed cell line which expresses Bombyx mori protein disulfide isomerase and human keratinocyte growth factor; and a step of producing the recombinant human keratinocyte growth factor.
Abstract translation: 目的：提供使用蚕豆蛋白二硫键异构酶基因制备重组人角质形成细胞生长因子的方法，以提高生产力。 构成：制备重组人角质形成细胞生长因子的方法包括：用含有该基因的重组表达载体转化昆虫细胞系的步骤; 用包含人角质形成细胞生长因子基因的重组载体同时或逐渐转化昆虫细胞系的步骤; 选择表达家蚕蛋白二硫键异构酶和人角质形成细胞生长因子的转化细胞系的步骤; 以及产生重组人角质形成细胞生长因子的步骤。
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公开(公告)号：KR101060910B1
公开(公告)日：2011-08-30
申请号：KR1020090040200
申请日：2009-05-08
Applicant: 한림대학교 산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: A61L27/3641 , A61F2/18 , A61F11/00 , A61F2002/183 , A61L27/3604 , A61L27/38 , A61L2430/14
Abstract: Provided are an artificial eardrum using silk protein and a method of fabricating the same. The artificial eardrum is fabricated in the form of a silk membrane by desalinating and drying silk protein (or silk fibroin) or a silk protein complex solution obtained after removal of sericin from a silkworm cocoon or silk fiber. Thus, regeneration of an eardrum perforated due to disease or a sudden accident is stimulated, a boundary of the regenerated eardrum is clean and biocompatibility and transparency are increased. In addition, the artificial eardrum may be fabricated using the silk protein or silk protein complex solution obtained from a silkworm cocoon alone or mixed with collagen, alginic acid, PEG or pluronic 127.
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公开(公告)号：KR1020110040389A
公开(公告)日：2011-04-20
申请号：KR1020090097627
申请日：2009-10-14
Applicant: 서울대학교산학협력단 , 대한민국(농촌진흥청장)
CPC classification number: A61L27/3604 , A61F2310/00796 , A61L27/32 , A61L27/56
Abstract: PURPOSE: A manufacturing method of a silk/hydroxyapatite complex nanofiber supporter for osteanagenesis is provided to efficiently coat the silk nanofiber supporter with hydroxyapatite in a short time. CONSTITUTION: A manufacturing method of a silk/hydroxyapatite complex nanofiber supporter for osteanagenesis comprises: a step of preparing silk spinning undiluted solution; a step of electrospinning the silk spinning undiluted solution; a step of dispersing the spun silk nanofiber; a step of manufacturing silk nanofiber having a sponge shape by freeze-drying the dispersed silk nanofiber; a step of coating the silk nanofiber with hydroxyapatite by dipping the silk nanofiber in simulated body fluid; and a step of forming pores on the silk nanofiber coated with hydroxyapatite.
Abstract translation: 目的：提供一种用于骨形成的丝/羟基磷灰石复合纳米纤维支持体的制造方法，以在短时间内有效地涂覆含有羟基磷灰石的丝绸纳米纤维支持体。 构成：用于骨形成的丝/羟基磷灰石复合纳米纤维支持体的制造方法包括：制备未纺丝的溶液的步骤; 静电纺丝未稀释溶液的步骤; 分散纺丝蚕丝纳米纤维的步骤; 通过将分散的蚕丝纳米纤维进行冷冻干燥来制造具有海绵形状的丝绸纳米纤维的步骤; 通过将丝素纳米纤维浸入模拟体液中，用羟基磷灰石涂覆蚕丝纳米纤维; 以及在涂有羟基磷灰石的丝素纳米纤维上形成孔的步骤。
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