Abstract:
The present invention relates to a method for detecting nucleic acid. According to an embodiment of the present invention, the method for detecting nucleic acid provides a method for detecting a target nucleic acid, polynucleotide, and compound. Accordingly, the method for detecting nucleic acid uses for detecting or quantifying the long target nucleic acid by preparation of ring DNA efficiently and sensitively.
Abstract:
A method and an apparatus for performing quantitative analysis of nucleic acids estimate the initial nucleic acid concentration of target nucleic acids by determining a curve-fitting area based on fluorescence intensity data obtained by performing PCR on the target nucleic acid; analyzing parameters from a result of performing PCR on a reference nucleic acid; and performing curve-fitting on the determined curve-fitting area using the analyzed parameters. [Reference numerals] (10) Nucleic acid quantitative analysis device; (110) Curve-fitting area determining unit; (120) Parameter analyzing unit; (130) Density estimator; (AA) PCR data; (BB) Initial nucleic acid concentration
Abstract:
PURPOSE: An apparatus for monitoring hybridization on a biochip in real time is provided to minimize wasting time and to optimize hybridization. CONSTITUTION: An apparatus(100) for monitoring hybridization of a biochip comprises: a chamber(30) of hybridization on the biochip; a prism(20) for providing light at the lower portion of the biochip to generate surface plasmon resonance; an optical detector(25) for detecting light reflected from the biochip; a processor(40) which controls hybridization and analyzes progress of hybridization based on the signal; a first probe region(15) having a probe for analysis; and a second probe region(16) having a probe for monitoring hybridization.
Abstract:
프로브를 이용하여 바이오 시료의 성분을 분석하는 바이오칩 및 그 제조 방법이 제공된다. 바이오칩은 기판, 기판 상면을 부분적으로 덮는 캡핑막 패턴, 및 캡핑막 패턴에 의해 노출된 기판 상면 상에 커플링된 복수의 프로브를 포함한다. 프로브, 캡핑막 패턴, 비프로브 셀 영역
Abstract:
A method for coating a silane layer on a substrate is provided to prevent misalignment between a substrate and other components in a manufacturing process of biochips. A method for coating a silane layer on a substrate comprises the following steps of: preparing the substrate(10); spin-coating the substrate with silane(300); and coupling a probe on a silane layer. The spin-coating includes the following steps of: coating a silane solution on the substrate; rotating the silane solution-coated substrate at a first speed and rotating again at a second speed; and solidifying the silane solution. The first speed is 500rpm, and the second speed is 2,000rpm.
Abstract:
A biochip for analyzing components of a biosample is provided to improve analysis reliability by preventing data noises and restraining an undesired coupling phenomenon of linkers or probes on a rear surface of a substrate. A biochip(11) comprises a substrate (100), a capping layer pattern(110), and a plurality of probes(140). The capping layer pattern partly covers a front surface of the substrate. The plurality of probes are coupled on the substrate exposed by the capping layer pattern. The substrate includes a plurality of probe cell areas(I) and non-probe cell areas(II). The non-probe cell areas separate the each probe cell area. The capping layer pattern is located on the non-probe cell area.