검출용 올리고머 및 이를 이용한 바이오 칩의 QC 방법
    45.
    发明授权
    검출용 올리고머 및 이를 이용한 바이오 칩의 QC 방법 有权
    检测器低聚物和质量控制方法的生物芯片使用相同

    公开(公告)号:KR101463874B1

    公开(公告)日:2014-12-05

    申请号:KR1020080007675

    申请日:2008-01-24

    CPC classification number: C12Q1/6837 C12Q2525/301 C12Q2525/131 C12Q2521/501

    Abstract: 검출용올리고머및 이를이용한바이오칩의 QC 방법이제공된다. 본발명의실시예들에따른검출용올리고머는특정서열의올리고머에상보적인서열을가지는주형및 주형의일단에연결된헤어핀을포함한다. 본발명의다른실시예들에따른검출용올리고머는특정서열의올리고머에상보적인서열을가지는단일가닥제1 올리고머및 제1 올리고머의일단에연결되고헤어핀구조를형성할수 있는서열을가지는단일가닥제2 올리고머를포함한다. 본발명의실시예들에따른바이오칩의 QC 방법은프로브의일단이고정층에커플링된바이오칩을제공하되, 프로브는원하는수의모노머를포함하는제1 프로브와원치않는수의모노머를포함하는제2 프로브를포함하고, 고정층에커플링된제1 및제2 프로브에 QC 프로브를혼성화하고, 1 프로브와 QC 프로브를연결하고, 제1 및제2 프로브와 QC 프로브의혼성화결합을절단하는것을포함하되, QC 프로브는제1 프로브의염기서열과상보적인염기서열을가지는주형및 주형의일단에연결된헤어핀을포함한다.

    실시간 PCR을 이용하여 핵산의 정량 분석을 수행하는 방법 및 장치
    46.
    发明公开
    실시간 PCR을 이용하여 핵산의 정량 분석을 수행하는 방법 및 장치 无效
    使用实时PCR进行核酸定量分析的方法和装置

    公开(公告)号:KR1020140002242A

    公开(公告)日:2014-01-08

    申请号:KR1020120070235

    申请日:2012-06-28

    Abstract: A method and an apparatus for performing quantitative analysis of nucleic acids estimate the initial nucleic acid concentration of target nucleic acids by determining a curve-fitting area based on fluorescence intensity data obtained by performing PCR on the target nucleic acid; analyzing parameters from a result of performing PCR on a reference nucleic acid; and performing curve-fitting on the determined curve-fitting area using the analyzed parameters. [Reference numerals] (10) Nucleic acid quantitative analysis device; (110) Curve-fitting area determining unit; (120) Parameter analyzing unit; (130) Density estimator; (AA) PCR data; (BB) Initial nucleic acid concentration

    Abstract translation: 用于进行核酸定量分析的方法和装置通过基于通过对靶核酸进行PCR得到的荧光强度数据确定曲线拟合面积来估计靶核酸的初始核酸浓度; 从对参照核酸进行PCR的结果分析参数; 并使用分析的参数在确定的曲线拟合区域上进行曲线拟合。 (附图标记)(10)核酸定量分析装置; (110)曲线拟合区域确定单元; (120)参数分析单元; (130)密度估计; (AA)PCR数据; (BB)初始核酸浓度

    바이오칩 상의 혼성화 반응을 모니터링하는 장치
    47.
    发明公开
    바이오칩 상의 혼성화 반응을 모니터링하는 장치 无效
    监测生物反应混合反应的装置

    公开(公告)号:KR1020100081540A

    公开(公告)日:2010-07-15

    申请号:KR1020090000827

    申请日:2009-01-06

    Abstract: PURPOSE: An apparatus for monitoring hybridization on a biochip in real time is provided to minimize wasting time and to optimize hybridization. CONSTITUTION: An apparatus(100) for monitoring hybridization of a biochip comprises: a chamber(30) of hybridization on the biochip; a prism(20) for providing light at the lower portion of the biochip to generate surface plasmon resonance; an optical detector(25) for detecting light reflected from the biochip; a processor(40) which controls hybridization and analyzes progress of hybridization based on the signal; a first probe region(15) having a probe for analysis; and a second probe region(16) having a probe for monitoring hybridization.

    Abstract translation: 目的:提供用于实时监测生物芯片杂交的装置,以最大限度地减少浪费时间并优化杂交。 构成:用于监测生物芯片的杂交的装置(100)包括:生物芯片上的杂交室(30); 棱镜(20),用于在所述生物芯片的下部提供光以产生表面等离子体共振; 用于检测从生物芯片反射的光的光学检测器(25); 处理器(40),其基于所述信号控制杂交并分析杂交进程; 具有用于分析的探针的第一探针区域(15) 和具有用于监测杂交的探针的第二探针区域(16)。

    기판 상에 실란층을 도포하는 방법
    49.
    发明公开
    기판 상에 실란층을 도포하는 방법 无效
    在衬底上涂层硅烷层的方法

    公开(公告)号:KR1020090022698A

    公开(公告)日:2009-03-04

    申请号:KR1020070088280

    申请日:2007-08-31

    CPC classification number: B01L9/527 B01L3/5027 B01L2300/16

    Abstract: A method for coating a silane layer on a substrate is provided to prevent misalignment between a substrate and other components in a manufacturing process of biochips. A method for coating a silane layer on a substrate comprises the following steps of: preparing the substrate(10); spin-coating the substrate with silane(300); and coupling a probe on a silane layer. The spin-coating includes the following steps of: coating a silane solution on the substrate; rotating the silane solution-coated substrate at a first speed and rotating again at a second speed; and solidifying the silane solution. The first speed is 500rpm, and the second speed is 2,000rpm.

    Abstract translation: 提供了在基板上涂布硅烷层的方法,以防止在生物芯片的制造过程中基板与其它部件之间的错位。 在衬底上涂覆硅烷层的方法包括以下步骤:制备衬底(10); 用硅烷(300)旋涂底物; 并将探针偶联在硅烷层上。 旋涂包括以下步骤:将硅烷溶液涂布在基材上; 以第一速度旋转硅烷溶液涂覆的基底并以第二速度再次旋转; 并固化硅烷溶液。 第一速度为500rpm,第二速度为2000rpm。

    바이오칩 및 그 제조 방법
    50.
    发明公开
    바이오칩 및 그 제조 방법 失效
    生物化学及其制备方法

    公开(公告)号:KR1020090021636A

    公开(公告)日:2009-03-04

    申请号:KR1020070086289

    申请日:2007-08-27

    Abstract: A biochip for analyzing components of a biosample is provided to improve analysis reliability by preventing data noises and restraining an undesired coupling phenomenon of linkers or probes on a rear surface of a substrate. A biochip(11) comprises a substrate (100), a capping layer pattern(110), and a plurality of probes(140). The capping layer pattern partly covers a front surface of the substrate. The plurality of probes are coupled on the substrate exposed by the capping layer pattern. The substrate includes a plurality of probe cell areas(I) and non-probe cell areas(II). The non-probe cell areas separate the each probe cell area. The capping layer pattern is located on the non-probe cell area.

    Abstract translation: 提供用于分析生物样品组分的生物芯片,以通过防止数据噪声并抑制衬底背面上的接头或探针的不期望的耦合现象来提高分析可靠性。 生物芯片(11)包括衬底(100),覆盖层图案(110)和多个探针(140)。 封盖层图案部分地覆盖基板的前表面。 多个探针耦合在由覆盖层图案暴露的基板上。 基板包括多个探针单元区域(I)和非探针单元区域(II)。 非探针单元区域分离每个探针单元区域。 覆盖层图案位于非探针单元区域上。

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