公开(公告)号：AT201768T

公开(公告)日：2001-06-15

申请号：AT96939709

申请日：1996-11-13

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J ,  OLEJNICZAK EDWARD T

IPC: G01R33/465 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/94 ,  G01R33/32 ,  G01R33/46 ,  G01N33/48 ,  G01N24/08

公开(公告)号：HU0003956A2

公开(公告)日：2001-03-28

申请号：HU0003956

申请日：1998-04-17

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J ,  OLEJNICZAK EDWARD T

IPC: G01R33/32 ,  A61K45/00 ,  C07K14/705 ,  C12N9/00 ,  C12Q1/68 ,  G01N24/08 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  G01R33/44

Abstract: The present invention provides a process for identifying compounds which bind to a specific target molecule. The process comprises the steps of: a) generating a first T2- or diffusion-filtered proton spectrum of one or a mixture of chemical compounds; b) exposing one or a mixture of chemical compounds to the target molecule; c) generating a second T2- or diffusion-filtered proton spectrum of one or a mixture of chemical compounds that has been exposed the target molecule in step (b); and d) comparing said first and second T2- or diffusion-filtered proton spectra to determine differences between said first and said second spectra, the differences identifying the presence of one or more compounds that are ligands which have bound to the target molecule.

公开(公告)号：CA2365385A1

公开(公告)日：2000-10-19

申请号：CA2365385

申请日：2000-03-21

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J

IPC: G01N24/12 ,  B08B9/04 ,  C07K1/13 ,  E21B37/04 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G01N33/542 ,  G01N33/566 ,  G01N33/68 ,  G01N33/94 ,  G01R33/46 ,  G01R33/465 ,  G01N24/08 ,  G01N33/58

Abstract: Methods of detecting binding of a putative ligand to a 13C-enriched target molecule, methods of screening for compounds which bind to a 13C-enriched target molecule, methods for calculating the dissociation constant of a liga nd compound which binds to a 13C-enriched target molecule, and methods employed in the determination of the specific amino acids in a 13C-enriched target molecule affected by the binding of a ligand, as well as compounds identifie d by these screening methods, are provided herewith.

公开(公告)号：ES2143793T3

公开(公告)日：2000-05-16

申请号：ES96940448

申请日：1996-11-13

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J

IPC: G01R33/465 ,  C12Q1/00 ,  G01N20060101 ,  G01N24/08 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  G01N33/68

Abstract: A method for designing and forming a ligand which binds to a specific target molecule comprises the steps of: (a) identifying a first ligand moiety that binds to the target molecule using two-dimensional N/ H NMR correlation spectroscopy; (b) identifying subsequent ligand moieties that bind to the target molecule using two-dimensional N/ H NMR correlation spectroscopy; (c) forming a complex of the first and subsequent ligand moieties to the target molecule; (d) determining the three-dimensional structure of the complex and, thus, the spatial orientation of the first and subsequent ligand moieties on the target molecule; and (e) linking the first and subsequent ligand moieties to form a new ligand to maintain the spatial orientation of the ligand moieties.

公开(公告)号：GR3032361T3

公开(公告)日：2000-04-27

申请号：GR20000400014

申请日：2000-01-14

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J

IPC: G01R33/465 ,  C12Q1/00 ,  G01N20060101 ,  G01N24/08 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  G01N33/68

Abstract: A method for designing and forming a ligand which binds to a specific target molecule comprises the steps of: (a) identifying a first ligand moiety that binds to the target molecule using two-dimensional N/ H NMR correlation spectroscopy; (b) identifying subsequent ligand moieties that bind to the target molecule using two-dimensional N/ H NMR correlation spectroscopy; (c) forming a complex of the first and subsequent ligand moieties to the target molecule; (d) determining the three-dimensional structure of the complex and, thus, the spatial orientation of the first and subsequent ligand moieties on the target molecule; and (e) linking the first and subsequent ligand moieties to form a new ligand to maintain the spatial orientation of the ligand moieties.

公开(公告)号：AT189063T

公开(公告)日：2000-02-15

申请号：AT96940448

申请日：1996-11-13

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J

IPC: G01R33/465 ,  C12Q1/00 ,  G01N20060101 ,  G01N24/08 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  G01N33/68

Abstract: A method for designing and forming a ligand which binds to a specific target molecule comprises the steps of: (a) identifying a first ligand moiety that binds to the target molecule using two-dimensional N/ H NMR correlation spectroscopy; (b) identifying subsequent ligand moieties that bind to the target molecule using two-dimensional N/ H NMR correlation spectroscopy; (c) forming a complex of the first and subsequent ligand moieties to the target molecule; (d) determining the three-dimensional structure of the complex and, thus, the spatial orientation of the first and subsequent ligand moieties on the target molecule; and (e) linking the first and subsequent ligand moieties to form a new ligand to maintain the spatial orientation of the ligand moieties.

公开(公告)号：AU711092B2

公开(公告)日：1999-10-07

申请号：AU7680496

申请日：1996-11-13

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J ,  OLEJNICZAK EDWARD T

IPC: G01R33/465 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  G01N33/94 ,  G01R33/32 ,  G01R33/46 ,  G01N33/48 ,  G01N24/08

公开(公告)号：CA2286795A1

公开(公告)日：1998-10-29

申请号：CA2286795

申请日：1998-04-17

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN ,  OLEJNICZAK EDWARD T ,  HAJDUK PHILIP J

IPC: G01R33/32 ,  A61K45/00 ,  C07K14/705 ,  C12N9/00 ,  C12Q1/68 ,  G01N24/08 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/68 ,  G01R33/44 ,  C12Q1/00 ,  G01R33/465 ,  G01N33/58

Abstract: The present invention provides a process for identifying compounds which bind to a specific target molecule. The process comprises the steps of: a) generating a first T2- or diffusion-filtered proton spectrum of one or a mixture of chemical compounds; b) exposing one or a mixture of chemical compounds to the target molecule; c) generating a second T2- or diffusionfiltered proton spectrum of one or a mixture of chemical compounds that has been exposed to the target molecule in step (b); and d) comparing said first and second T2- or diffusion-filtered proton spectra to determine differences between said first and said second spectra, the differences identifying the presence of one or more compounds that are ligands which have bound to the target molecule.

公开(公告)号：CA2653517A1

公开(公告)日：1997-05-22

申请号：CA2653517

申请日：1996-11-13

Applicant: ABBOTT LAB

Inventor： HAJDUK PHILIP J ,  OLEJNICZAK EDWARD T ,  FESIK STEPHEN W

IPC: G01N33/15 ,  G01N24/08 ,  G01N33/53 ,  G01N33/68 ,  G01N33/94 ,  G01R33/46 ,  G06F19/00

Abstract: The present invention provides a process of designing compounds which bind t o a specific target molecule. The process includes the steps of a) identifying a first ligand to the target molecule using two-dimensional 15N/1H NMR correlation spectroscop y; b) identifying a second ligand to the target molecule using two-dimensional 15N/1H NMR correlation spectroscopy; c) forming a ternary complex by binding the first and second ligands to the target molecule; d) determining the three-dimensional structu re of the ternary complex and thus the spatial orientation of the first and second ligands on the target molecule; and e) linking the first and second ligands to form the dru g, wherein the spatial orientation of step (d) is maintained.

公开(公告)号：CA2237343A1

公开(公告)日：1997-05-22

申请号：CA2237343

申请日：1996-11-13

Applicant: ABBOTT LAB

Inventor： FESIK STEPHEN W ,  HAJDUK PHILIP J

IPC: G01R33/465 ,  C12Q1/00 ,  G01N20060101 ,  G01N24/08 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/542 ,  G01N33/566 ,  G01N33/58 ,  G01N33/68

Abstract: The present invention provides a process for identifying compounds which bind to a specific target molecule. The process includes the steps of: a) generating a first two-dimensional 15N/1H NMR correlation spectrum of a 15Nlabeled target molecule; b) exposing the labeled target molecule to one or a mixture of chemical compounds; c) generating a second two-dimensional 15N/1H NMR correlation spectrum of the labeled target molecule that has been exposed to one or a mixture of compounds in step (b); and d) comparing said first and second two-dimensional 15N/1H NMR correlation spectra to determine differences between said first and said second spectra, the differences indentifying the presence of one or more compounds that are ligands which have bound to the target molecule.