公开(公告)号：CA2434518A1

公开(公告)日：2002-07-25

申请号：CA2434518

申请日：2002-01-19

Applicant: BASF AG

Inventor： HERMANN THERON ,  PATTERSON THOMAS A ,  YOCUM ROGERS R ,  BALDENIUS KAI-UWE ,  PERO JANICE G ,  BECK CHRISTINE

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12P7/42 ,  C12P13/02 ,  C12P13/04 ,  C12R1/125 ,  C12N15/03 ,  C12N15/52

Abstract: The present invention features methods of producting 3-(2-hydroxy-3-methyl- butyrylamino)-propionic acid ("HMBPA") and .alpha.-hydroxyisovalerate (".alpha.-HIV") utilizing microorganisms having modified pantothenate biosynthetic enzyme activities. Recombinant microorganisms and conditions fo r culturing same are also featured. Also featured are compositions including HMBPA and compositions including .alpha.-HIV.

公开(公告)号：NO20021382L

公开(公告)日：2002-05-16

申请号：NO20021382

申请日：2002-03-20

Applicant: BASF AG

Inventor： YOCUM R ROGERS ,  PATTERSON THOMAS A ,  HERMANN THERON ,  PERO JANICE G

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12P13/02 ,  C12P13/04 ,  C12P13/06 ,  C12P17/04 ,  C12Q1/48 ,  C12R1/125 ,  C12P21/00 ,  C12N15/31

Abstract: The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzyme A biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate ±-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX , as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

公开(公告)号：NO20021382A

公开(公告)日：2002-05-16

申请号：NO20021382

申请日：2002-03-20

Applicant: BASF AG

Inventor： YOCUM R ROGERS ,  PATTERSON THOMAS A ,  HERMANN THERON ,  PERO JANICE G

IPC: C12N15/09 ,  C12N1/21 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12P13/02 ,  C12P13/04 ,  C12P13/06 ,  C12P17/04 ,  C12Q1/48 ,  C12R1/125 ,  C12P21/00 ,  C12N15/31

CPC classification number: C12P13/02 ,  C12N9/0006 ,  C12N9/1014 ,  C12N9/1205 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/42 ,  C12P13/06 ,  C12P17/04