公开(公告)号：CA2096389A1

公开(公告)日：1994-01-18

申请号：CA2096389

申请日：1993-05-17

Applicant: BECKMAN INSTRUMENTS INC

Inventor： LIU CHENG-MING ,  WANG HANN-PING ,  STERNBERG JAMES C ,  CHEN FU-TAI A ,  KLEIN GERALD L

IPC: G01N27/447 ,  G01N33/53 ,  G01N33/561 ,  G01N33/537 ,  G01N33/577 ,  G01N33/68

Abstract: Disclosed herein are methodologies for the Capillary Electrophoretic Immunosubtraction ("CEI") of samples. In a preferred embodiment, CEI of a sample comprising constituent parts to be separated comprises tare steps of: (1) separating a first aliquot of the sample into constituent parts by capillary electrophoretic techniques, and detecting said parts; (2) admixing a second aliquot of said sample with at least one specific binding partner to a constituent of said sample, said specific binding partner capable of being substantially removed from said aliquot; (3) separating said second aliquot into constituent parts by capillary electrophoretic techniques and detecting said parts; and (4) comparing the separated constituent parts of step (3) with the separated constituent parts of step (1).

公开(公告)号：DK157711C

公开(公告)日：1990-07-09

申请号：DK487584

申请日：1984-10-11

Applicant: BECKMAN INSTRUMENTS INC

Inventor： BUZZA EDMUND E ,  STERNBERG JAMES C

IPC: G01N20060101 ,  G01N27/42

公开(公告)号：CH611422A5

公开(公告)日：1979-05-31

申请号：CH744576

申请日：1976-06-11

Applicant: BECKMAN INSTRUMENTS INC

Inventor： BUZZA EDMUND E ,  STERNBERG JAMES C

IPC: G01N27/416 ,  G01N33/487 ,  G01N33/49 ,  G01N27/56 ,  G01N33/16

Abstract: Apparatus for measuring carbon dioxide and chloride in blood. A blood sample is reacted with acid in a sample container to release carbon dioxide, a portion of which diffuses through a gas-permeable membrane and reacts with an electrolyte to change the pH thereof. A pair of pH measuring electrodes monitor the electrolyte pH at respective locations adjacent to and remote from the region of reaction within the electrolyte. The pH electrodes are coupled to respective input terminals of a differential amplifier to derive a differential pH signal. The differential pH signal is differentiated to derive a rate of change of pH output signal which is measured to indicate the concentration of carbon dioxide. The pH measuring electrodes are in electrolytic communication and means is provided to renew the electrolyte between measurements. Coulometric measurement of chloride in the sample is performed simultaneously with the carbon dioxide measurement.

公开(公告)号：AU1468276A

公开(公告)日：1977-12-15

申请号：AU1468276

申请日：1976-06-07

Applicant: BECKMAN INSTRUMENTS INC

Inventor： BUZZA EDMUND E ,  STERNBERG JAMES C

IPC: G01N27/416 ,  G01N33/487 ,  G01N33/49 ,  G01N27/58 ,  G01N27/30 ,  G01N27/40 ,  G01N33/16

Abstract: Apparatus for measuring carbon dioxide and chloride in blood. A blood sample is reacted with acid in a sample container to release carbon dioxide, a portion of which diffuses through a gas-permeable membrane and reacts with an electrolyte to change the pH thereof. A pair of pH measuring electrodes monitor the electrolyte pH at respective locations adjacent to and remote from the region of reaction within the electrolyte. The pH electrodes are coupled to respective input terminals of a differential amplifier to derive a differential pH signal. The differential pH signal is differentiated to derive a rate of change of pH output signal which is measured to indicate the concentration of carbon dioxide. The pH measuring electrodes are in electrolytic communication and means is provided to renew the electrolyte between measurements. Coulometric measurement of chloride in the sample is performed simultaneously with the carbon dioxide measurement.

公开(公告)号：CA826397A

公开(公告)日：1969-11-04

申请号：CA826397D

Applicant: BECKMAN INSTRUMENTS INC

Inventor： STERNBERG JAMES C ,  KRULL IRWIN H

公开(公告)号：AU698206B2

公开(公告)日：1998-10-29

申请号：AU3243195

申请日：1995-08-10

Applicant: BECKMAN INSTRUMENTS INC

Inventor： REDDY M PARAMESWARA ,  STERNBERG JAMES C

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/543 ,  G01N33/547 ,  G01N33/566 ,  G01N21/64

Abstract: Double stranded nucleic acid duplexes serve as universal harvestable and cleavable link systems in a variety of different types of immunoassays (e.g., sandwich, competitive, etc.). Depending upon the type of assay, at least one specific component involved in the assay system is attached to a first member of a pair of sequences forming a double stranded nucleic acid (i.e., two oligonucleotides comprising substantially complementary sequences). The assay is carried out in the presence of a support to which is attached an oligonucleotide which is the other member of the pair of sequences forming a double-stranded nucleic acid duplex under hybridization conditions. Upon the hybridization of the two complementary oligonucleotides to form a duplex, the component of the assay system to which the first member of the pair of oligonucleotides is attached may thereby be effectively removed from the solution phase and harvested onto the support. Oligonucleotides bound to a support are reusable in multiple successive assays. Moreover, any given support-bound oligonucleotide can be used in accordance with the present invention for the analysis of a variety of different analytes. In many cases, the assay system includes a label to facilitate quantifying the amount of analyte; in others, the amount of analyte may be determined without the use of any extraneous label.

公开(公告)号：DE69320273D1

公开(公告)日：1998-09-17

申请号：DE69320273

申请日：1993-05-17

Applicant: BECKMAN INSTRUMENTS INC

Inventor： LIU CHING-MING ,  WANG HANN-PING ,  CHEN FU-TAI ALBERT ,  STERNBERG JAMES C ,  KLEIN GERALD L

IPC: G01N27/447 ,  G01N33/53 ,  G01N33/561 ,  G01N33/68

Abstract: Disclosed herein are methodologies for the Capillary Electrophoretic Immunosubtraction ("CEI") of samples. In a preferred embodiment, CEI of a sample comprising constituent parts to be separated comprises the steps of: (1) separating a first aliquot of the sample into constituent parts by capillary electrophoretic techniques, and detecting said parts; (2) admixing a second aliquot of said sample with at least one specific binding partner to a constituent of said sample, said specific binding partner capable of being substantially removed from said aliquot; (3) separating said second aliquot into constituent parts by capillary electrophoretic techniques and detecting said parts; and (4) comparing the separated constituent parts of step (3) with the separated constituent parts of step (1).

公开(公告)号：AT169737T

公开(公告)日：1998-08-15

申请号：AT93303805

申请日：1993-05-17

Applicant: BECKMAN INSTRUMENTS INC

Inventor： LIU CHING-MING ,  WANG HANN-PING ,  CHEN FU-TAI ALBERT ,  STERNBERG JAMES C ,  KLEIN GERALD L

IPC: G01N27/447 ,  G01N33/53 ,  G01N33/561 ,  G01N33/68

Abstract: Disclosed herein are methodologies for the Capillary Electrophoretic Immunosubtraction ("CEI") of samples. In a preferred embodiment, CEI of a sample comprising constituent parts to be separated comprises the steps of: (1) separating a first aliquot of the sample into constituent parts by capillary electrophoretic techniques, and detecting said parts; (2) admixing a second aliquot of said sample with at least one specific binding partner to a constituent of said sample, said specific binding partner capable of being substantially removed from said aliquot; (3) separating said second aliquot into constituent parts by capillary electrophoretic techniques and detecting said parts; and (4) comparing the separated constituent parts of step (3) with the separated constituent parts of step (1).

公开(公告)号：ES2100285T3

公开(公告)日：1997-06-16

申请号：ES92303985

申请日：1992-05-01

Applicant: BECKMAN INSTRUMENTS INC

Inventor： CHEN FU-TAI ALBERT ,  STERNBERG JAMES C

IPC: B01D57/02 ,  C07K1/26 ,  G01N27/447 ,  G01N33/66 ,  G01N33/68 ,  G01N33/92 ,  G01N27/26 ,  G01N33/50

公开(公告)号：AU3243195A

公开(公告)日：1996-03-22

申请号：AU3243195

申请日：1995-08-10

Applicant: BECKMAN INSTRUMENTS INC

Inventor： REDDY M PARAMESWARA ,  STERNBERG JAMES C

IPC: G01N33/53 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6834 ,  G01N33/543 ,  G01N33/547 ,  G01N33/566 ,  G01N21/64

Abstract: Double stranded nucleic acid duplexes serve as universal harvestable and cleavable link systems in a variety of different types of immunoassays (e.g., sandwich, competitive, etc.). Depending upon the type of assay, at least one specific component involved in the assay system is attached to a first member of a pair of sequences forming a double stranded nucleic acid (i.e., two oligonucleotides comprising substantially complementary sequences). The assay is carried out in the presence of a support to which is attached an oligonucleotide which is the other member of the pair of sequences forming a double-stranded nucleic acid duplex under hybridization conditions. Upon the hybridization of the two complementary oligonucleotides to form a duplex, the component of the assay system to which the first member of the pair of oligonucleotides is attached may thereby be effectively removed from the solution phase and harvested onto the support. Oligonucleotides bound to a support are reusable in multiple successive assays. Moreover, any given support-bound oligonucleotide can be used in accordance with the present invention for the analysis of a variety of different analytes. In many cases, the assay system includes a label to facilitate quantifying the amount of analyte; in others, the amount of analyte may be determined without the use of any extraneous label.