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    1.
    发明专利
    未知

    公开(公告)号：AT152775T

    公开(公告)日：1997-05-15

    申请号：AT92925261

    申请日：1992-11-16

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI A ,  STERNBERG JAMES C ,  KLIEN GERALD ,  LIU CHENG-MING

    IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447 ,  C12Q1/32 ,  C12Q1/50

    Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

    2.
    发明专利
    未知

    公开(公告)号：AT134039T

    公开(公告)日：1996-02-15

    申请号：AT93302616

    申请日：1993-04-02

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： CHEN FU-TAI A

    IPC: C07K14/00 ,  C07K1/26 ,  C07K14/805 ,  G01N27/447 ,  G01N33/72

    Abstract: Disclosed herein are buffers and methodologies for the capillary zone electrophoretic analysis of hemoglobin and the variants of hemoglobin. In a particularly preferred embodiment, the buffer comprises at least about 100mM of barbituric acid, derivatives of barbituric acid, combinations of barbituric acid and the derivatives of barbituric acid, and combinations of the derivatives of barbituric acid, and has a pH of at least about 8.0.

    4.
    发明专利
    未知

    公开(公告)号：DE69301491T2

    公开(公告)日：1996-06-27

    申请号：DE69301491

    申请日：1993-04-02

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： CHEN FU-TAI A

    IPC: C07K14/00 ,  C07K1/26 ,  C07K14/805 ,  G01N27/447 ,  G01N33/72

    Abstract: Disclosed herein are buffers and methodologies for the capillary zone electrophoretic analysis of hemoglobin and the variants of hemoglobin. In a particularly preferred embodiment, the buffer comprises at least about 100mM of barbituric acid, derivatives of barbituric acid, combinations of barbituric acid and the derivatives of barbituric acid, and combinations of the derivatives of barbituric acid, and has a pH of at least about 8.0.

    5.
    发明专利
    未知

    公开(公告)号：ES2086190T3

    公开(公告)日：1996-06-16

    申请号：ES93302616

    申请日：1993-04-02

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： CHEN FU-TAI A

    IPC: C07K14/00 ,  C07K1/26 ,  C07K14/805 ,  G01N27/447 ,  G01N33/72

    Abstract: Disclosed herein are buffers and methodologies for the capillary zone electrophoretic analysis of hemoglobin and the variants of hemoglobin. In a particularly preferred embodiment, the buffer comprises at least about 100mM of barbituric acid, derivatives of barbituric acid, combinations of barbituric acid and the derivatives of barbituric acid, and combinations of the derivatives of barbituric acid, and has a pH of at least about 8.0.

    6.
    发明专利
    未知

    公开(公告)号：DE69301491D1

    公开(公告)日：1996-03-21

    申请号：DE69301491

    申请日：1993-04-02

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： CHEN FU-TAI A

    IPC: C07K14/00 ,  C07K1/26 ,  C07K14/805 ,  G01N27/447 ,  G01N33/72

    Abstract: Disclosed herein are buffers and methodologies for the capillary zone electrophoretic analysis of hemoglobin and the variants of hemoglobin. In a particularly preferred embodiment, the buffer comprises at least about 100mM of barbituric acid, derivatives of barbituric acid, combinations of barbituric acid and the derivatives of barbituric acid, and combinations of the derivatives of barbituric acid, and has a pH of at least about 8.0.

    7.
    发明专利
    未知

    公开(公告)号：ES2102533T3

    公开(公告)日：1997-08-01

    申请号：ES92925261

    申请日：1992-11-16

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： HSIEH YOU-ZUNG ,  CHEN FU-TAI A ,  STERNBERG JAMES C ,  KLIEN GERALD ,  LIU CHENG-MING

    IPC: C12Q1/04 ,  C12Q1/00 ,  G01N27/447

    Abstract: Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.

    ANALYSIS OF SAMPLES BY CAPILLARY ELECTROPHORETIC IMMUNOSUBTRACTION
    8.
    发明专利
    ANALYSIS OF SAMPLES BY CAPILLARY ELECTROPHORETIC IMMUNOSUBTRACTION 未知

    公开(公告)号：CA2096389A1

    公开(公告)日：1994-01-18

    申请号：CA2096389

    申请日：1993-05-17

    Applicant: BECKMAN INSTRUMENTS INC

    Inventor： LIU CHENG-MING ,  WANG HANN-PING ,  STERNBERG JAMES C ,  CHEN FU-TAI A ,  KLEIN GERALD L

    IPC: G01N27/447 ,  G01N33/53 ,  G01N33/561 ,  G01N33/537 ,  G01N33/577 ,  G01N33/68

    Abstract: Disclosed herein are methodologies for the Capillary Electrophoretic Immunosubtraction ("CEI") of samples. In a preferred embodiment, CEI of a sample comprising constituent parts to be separated comprises tare steps of: (1) separating a first aliquot of the sample into constituent parts by capillary electrophoretic techniques, and detecting said parts; (2) admixing a second aliquot of said sample with at least one specific binding partner to a constituent of said sample, said specific binding partner capable of being substantially removed from said aliquot; (3) separating said second aliquot into constituent parts by capillary electrophoretic techniques and detecting said parts; and (4) comparing the separated constituent parts of step (3) with the separated constituent parts of step (1).

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