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公开(公告)号：WO2008070469A2
公开(公告)日：2008-06-12
申请号：PCT/US2007085496
申请日：2007-11-26
Applicant: BIO RAD LABORATORIES , RAGSDALE CHARLES W
Inventor： RAGSDALE CHARLES W
Abstract: Systems, methods and apparatus provide flexible and efficient high throughput electroporation systems. An electrical pulse may be transmitted to any number of channels of a multi-channel sample plate. Drivers can provide the selection of which channels to transmit the electrical pulse. To provide efficient transitions between electrical pulses, discharge circuits provide efficient means achieve a desired voltage.
Abstract translation: 系统，方法和设备提供灵活且高效的高通量电穿孔系统。 电脉冲可以传输到多通道样品板的任何数量的通道。 驾驶员可以选择传输电脉冲的通道。 为了在电脉冲之间提供有效的转换，放电电路提供实现期望电压的有效手段。
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公开(公告)号：WO2005104688A3
公开(公告)日：2006-07-27
申请号：PCT/US2005012573
申请日：2005-04-13
Applicant: BIO RAD LABORATORIES , RAGSDALE CHARLES W
Inventor： RAGSDALE CHARLES W
CPC classification number: G01N21/6452 , G01N2021/4709 , G01N2021/6419 , G01N2021/6471 , G01N2201/067 , G01N2201/0691
Abstract: A method is provided for measuring a response to a stimulus of a plurality of samples spots of a sample using a measuring system having a measurement range to generate an image of the sample in digital space. The method includes, for each sample, while measuring the response, varying the stimulus to include at least one stimulus value where the measured response corresponds to a value in an intermediate portion of the measuring range, and storing a value of the measured response that corresponds to a value in the intermediate portion of the measurement range, and the stimulus value that produced that value of the measured response.
Abstract translation: 提供一种用于使用具有测量范围的测量系统来测量对样本的多个采样点的刺激的响应的方法，以在数字空间中生成样本的图像。 该方法包括对于每个样本，在测量响应的同时，改变刺激以包括至少一个刺激值，其中测量的响应对应于测量范围的中间部分中的值，并且存储对应于测量范围的测量响应的值 到测量范围的中间部分中的值，以及产生测量响应值的刺激值。
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公开(公告)号：CA2663155C
公开(公告)日：2013-03-19
申请号：CA2663155
申请日：2007-09-20
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: Systems, methods, apparatus, and circuits for controlling an electrical signal transmitted to a sample load are provided. The electrical signal produced by a capacitor is controlled via a control signal sent to a variable resistance device that is connected in parallel with the sample load. The variable resistance device includes a resistance and a switch in series. The control signal opens and closes the switch, thus providing a variable resistance based on the amount of time the switch is closed.
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公开(公告)号：CA2641488C
公开(公告)日：2011-10-25
申请号：CA2641488
申请日：2007-01-26
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W , FULLER JOHN M , STEPHENS NICHOLAS R
IPC: C12M1/42
Abstract: An electroporator for high-throughput electroporation is constructed with a well plate in which each well has internal electrodes that extend beyond the opening of the well to form contact areas, either as horizontal platforms extending laterally from the well rims or as extended heights of thin electrode plates. The electroporator also includes a lid that contains circuitry and electrical contacts that mate with the exposed contact areas in the well plate. The interchangeability of lids allows the wells to be shocked according to different protocols.
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公开(公告)号：CA2723595A1
公开(公告)日：2009-11-19
申请号：CA2723595
申请日：2009-05-08
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: Adherent cells and other membranous structures that are immobilized on a solid surface are transfected by electroporation in which the electric field is produced by a array of closely spaced electrodes positioned above the surface. Each electrode is substantially smaller in at least one lateral dimension than the dimensions of a single cell, and the electrodes in each pair are spaced apart by distances selected such that a maximum of one cell will reside within the field produced by each pair, and the distance of the electrodes above the surface to which the cells are adherent is small enough to place the cell within the resulting electric field and yet great enough to avoid contact of the electrodes with the cell membrane.
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Patent Agency Ranking
公开(公告)号：CA2715790A1
公开(公告)日：2009-08-27
申请号：CA2715790
申请日：2009-02-11
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: An electroporation cuvette is constructed with electroporation electrodes arranged in non-parallel relation to form a gap whose width varies with the location within the cuvette, plus a pair of positioning electrodes that are arranged to cause electrophoretic migration of biological cells within the cuvette according to cell size. Once the cells, suspended in a solution of the impregnant, are distributed in the cuvette by the positioning electrodes, electric field pulses are generated by the non-parallel electroporation electrodes. Because of their distribution in the cuvette, the various cells will experience voltage differentials across their widths that approach uniformity regardless of cell diameter, since the larger cells will be positioned at locations where the gap between the electrodes is greater and the smaller cells at locations where the gap is relatively small while the voltage drop across the entire gap is uniform along the length of the cell. The voltage differential across the width of the cell is thus roughly paired with the cell diameter, and this reduces the disparity in voltage differential that cells of different sizes would otherwise experience with parallel electrodes.
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公开(公告)号：CA2670933A1
公开(公告)日：2008-06-12
申请号：CA2670933
申请日：2007-11-26
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: Systems, methods and apparatus provide flexible and efficient high throug hput electroporation systems. An electrical pulse may be transmitted to any number of channels of a multi-channel sample plate. Drivers can provide the selection of which channels to transmit the electrical pulse. To provide eff icient transitions between electrical pulses, discharge circuits provide eff icient means achieve a desired voltage.
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公开(公告)号：CA2663709A1
公开(公告)日：2008-03-27
申请号：CA2663709
申请日：2007-09-20
Applicant: BIO RAD LABORATORIES
Inventor： KULISCH STEVEN , BOUCHARD GARY , COLLINS MICHELLE , RAGSDALE CHARLES W , RUBIO TERESA
Abstract: A chamber that includes electrical contacts and a protective lid with a m anually operated release is designed to receive a multi-well electroporation plate and to allow high-throughput electroporation on the well contents wit h minimal risk of electrical shock to the user and minimal opportunity for s ample loss and contamination.
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公开(公告)号：DE69535341T2
公开(公告)日：2007-06-28
申请号：DE69535341
申请日：1995-06-13
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: A method for operating a transfection system adapted to deliver a prespecified high-voltage current to a chemical solution, comprising the steps of: asserting a trigger signal when power is removed from the transfection system; triggering a crowbar circuit coupled to a charge storage device that provides the high-voltage current when said trigger signal is asserted; and discharging said charge storage device through said crowbar circuit when said crowbar circuit is activated. An apparatus for directing a first predetermined current profile at a second predetermined high voltage level to a chemical solution is also disclosed, along with a hi-joule switch and a capacitor expansion module.
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公开(公告)号：DE69535341D1
公开(公告)日：2007-01-25
申请号：DE69535341
申请日：1995-06-13
Applicant: BIO RAD LABORATORIES
Inventor： RAGSDALE CHARLES W
Abstract: A method for operating a transfection system adapted to deliver a prespecified high-voltage current to a chemical solution, comprising the steps of: asserting a trigger signal when power is removed from the transfection system; triggering a crowbar circuit coupled to a charge storage device that provides the high-voltage current when said trigger signal is asserted; and discharging said charge storage device through said crowbar circuit when said crowbar circuit is activated. An apparatus for directing a first predetermined current profile at a second predetermined high voltage level to a chemical solution is also disclosed, along with a hi-joule switch and a capacitor expansion module.
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