中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

9 records (took 0.024 seconds)

    HIGH EFFICIENCY ELECTROPORATION BUFFER
    1.
    发明专利
    HIGH EFFICIENCY ELECTROPORATION BUFFER 未知

    公开(公告)号：CA2685409A1

    公开(公告)日：2008-11-06

    申请号：CA2685409

    申请日：2008-04-04

    Applicant: BIO RAD LABORATORIES

    Inventor： TEREFE JOSEPH ,  RUBIO TERESA

    IPC: C12N5/00 ,  C12N13/00 ,  C12N15/87

    Abstract: Electroporation of mammalian cells is performed to high efficiency by use of a buffer solution containing trehalose, sucrose, or both, in addition to an inorganic phosphate buffer, an organic sulfonic acid buffer, a halide sa lt of an alkali or alkaline earth metal, a chelating agent, an alkyl methyl sulfoxide, and a nucleotide triphosphate, with substantially no sodium. This buffer solution is effective both in the electroporation of cells that are suspended in the buffer solution and in the electroporation of cells that ar e fixed on a surface.

    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW
    2.
    发明专利
    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW 未知

    公开(公告)号：CA2677171A1

    公开(公告)日：2008-08-21

    申请号：CA2677171

    申请日：2008-02-06

    Applicant: BIO RAD LABORATORIES

    Inventor： RUBIO TERESA ,  RAGSDALE CHARLES W

    IPC: A61K48/00 ,  C07H21/04 ,  C12N15/87

    Abstract: Biological cells and other membranous structures are transfected in a flo w-through system by first rendering the structures magnetically active such that they respond to a magnetic field, suspending the structures in a soluti on of an exogenous species with which the structures are to be transfected, then placing the suspension in a channel and using a moving magnetization pa ttern along the channel wall to cause the structures to travel through the c hannel. Along their path of travel, the structures pass a transmitter that e mits transfection energy sufficient to cause the exogenous species in the su spension to permeate the structure membranes and enter the interiors of the structures.

    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW
    5.
    发明申请
    TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW 审中-公开
    磁力传递连续流量的转移

    公开(公告)号：WO2008100749A3

    公开(公告)日：2008-11-20

    申请号：PCT/US2008053135

    申请日：2008-02-06

    Applicant: BIO RAD LABORATORIES ,  RAGSDALE CHARLES W ,  RUBIO TERESA

    Inventor： RAGSDALE CHARLES W ,  RUBIO TERESA

    IPC: A61K48/00 ,  C07H21/04 ,  C12N15/87

    CPC classification number: C12N13/00 ,  C12M35/02 ,  C12M35/06 ,  C12N15/85 ,  C12N15/87

    Abstract: Biological cells and other membranous structures are transfected in a flow-through system by first rendering the structures magnetically active such that they respond to a magnetic field, suspending the structures in a solution of an exogenous species with which the structures are to be transfected, then placing the suspension in a channel and using a moving magnetization pattern along the channel wall to cause the structures to travel through the channel. Along their path of travel, the structures pass a transmitter that emits transfection energy sufficient to cause the exogenous species in the suspension to permeate the structure membranes and enter the interiors of the structures.

    Abstract translation: 生物细胞和其他膜结构在流通系统中转染，首先使结构具有磁性活性，使得它们响应于磁场，将结构悬浮在结构待转染的外源物质的溶液中，然后 将悬架放置在通道中并且沿着通道壁使用移动的磁化模式以使结构行进通过通道。 沿着他们的旅行路线，结构通过发射器，其发射足够的转染能量，使得悬浮液中的外源物质渗入结构膜并进入结构的内部。

Patent Agency Ranking