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公开(公告)号：WO0025171A2
公开(公告)日：2000-05-04
申请号：PCT/DE9903400
申请日：1999-10-25
Applicant: LEICA MICROSYSTEMS , ENGELHARDT JOHANN
Inventor： ENGELHARDT JOHANN
CPC classification number: G02B21/0076 , G02B21/0048 , G02B21/006 , G02B21/0064 , G02B26/101
Abstract: An arrangement for optically scanning an object, more particularly used in preferably confocal laser scan microscopy, whereby a lens or an objective (3) and at least one scanning mirror (4) are arranged inside the the illuminating /detecting beam (1,2). In order to scan object fields whose size is greater than the optic system of the microscope with sufficiently rapid acquisition of data, using simple optical components, the invention is characterized in that the mirror (4) can rotate or pivot around an axis of rotation (5) that is at least substantially orthagonal with respect to the scanning surface of the object.
Abstract translation: 在优选的共焦激光扫描显微镜光学扫描的物体，特别是，其特征在于，被布置在照明/检测光束路径（1，2）一个透镜或一个透镜（3）和至少一个扫描镜（4）的装置，是用于扫描 具有足够快速的数据采集并使用简单的光学部件的显微镜镜片边界物场的物场尺寸，其特征在于，具有至少基本上正交的旋转轴（5）的扫描物体的镜（4）旋转或枢转。
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公开(公告)号：WO9959228A3
公开(公告)日：2000-02-17
申请号：PCT/DE9901386
申请日：1999-05-07
Applicant: LEICA MICROSYSTEMS , ENGELHARDT JOHANN , ZAPF THOMAS , HUG BILL
Inventor： ENGELHARDT JOHANN , ZAPF THOMAS , HUG BILL
CPC classification number: H01S3/10 , G02B21/0032 , G02B21/0036 , G02B21/0084
Abstract: The invention relates to a method for operating a laser light source, more particularly, for scanning in a confocal laser scan microscope, whereby the laser light source (3) is supplied with power by means of a power unit. In order to enhance service life of the laser light source (3) and reduce operating costs, the laser light source (3) is operated with the required power almost exclusively during effective beam utilization time, preferably during data capture. By using a pulsating laser light source, effective beam utilization time, especially data capture, is synchronized (14) substantially with the emission cycle of the laser light source.
Abstract translation: 在共聚焦激光扫描显微镜，其中，所述激光光源（3）经由电源与电流供电操作激光光源，尤其是用于扫描，在延伸的激光光源（3），并减少在所述激光光源的运行成本的寿命的方法 （3）基本上只Strahlnutzzeit期间，优选地在数据采集期间，与所要求的性能操作。 当使用脉冲激光光源是Strahlnutzzeit，特别是数据采集，基本上与激光光源的发光周期是同步的（14）一致。
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公开(公告)号：WO9942885A3
公开(公告)日：1999-09-23
申请号：PCT/DE9900480
申请日：1999-02-22
Applicant: LEICA MICROSYSTEMS , ENGELHARDT JOHANN
Inventor： ENGELHARDT JOHANN
IPC: G02B21/00
CPC classification number: G02B21/0052 , G02B21/0024 , G02B21/0056
Abstract: The invention relates to a system or a method for calibrating a preferably confocal laser scanning microscope with which an object (1) can be scanned by a scanning beam (2). The aim of the invention is to simplify the calibration process so that it can be carried out as often as required. To this end, the inventive system is characterised by calibrating elements (12) which are situated in the plane of an intermediate image and which can also be scanned by the scanning beam (2).
Abstract translation: 的装置以及用于从扫描光束（2）被扫描校准优选共焦激光扫描显微镜，一个对象（1）的方法是，为了容易和从而尽可能经常可行的校准中的中间图像的平面，其特征在于（11）布置的校准装置（ 12），这也（通过扫描光束2）可以被扫描。
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公开(公告)号：DE10029167B8
公开(公告)日：2015-07-02
申请号：DE10029167
申请日：2000-06-19
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN
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公开(公告)号：DE59915085D1
公开(公告)日：2009-11-05
申请号：DE59915085
申请日：1999-01-28
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN , ULRICH HEINRICH , GUGEL HILMAR
Abstract: The invention relates to an optical arrangement provided for a spectral fanning out of a light beam (1), preferably the detection beam path of a confocal microscope, especially for the subsequent splitting of the fanned out beam (2) out of the dispersion plane thereof. The optical arrangment is also provided for detecting the fanned out spectral regions (4), whereby the incoming light beam (1) is focused on a pinhole (7). The invention is characterized in that the pinhole (7) has a polygonal passageway (8) in order to realize a high dynamic response when the light beam is split into spectral regions (4) or into spectral colors.
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Patent Agency Ranking
公开(公告)号：DE10102033B4
公开(公告)日：2008-07-10
申请号：DE10102033
申请日：2001-01-18
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN
IPC: G02B21/00
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公开(公告)号：DE10150542B4
公开(公告)日：2007-03-29
申请号：DE10150542
申请日：2001-10-12
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN , HOFFMANN JUERGEN
Abstract: The invention discloses a fluorescence microscope comprising a light source that emits excitation light for illumination of a specimen, means for defining a two-dimensional search region for the excitation and detection wavelengths, means for selecting a subregion from the search region, at least one detector that detects detected light proceeding from the specimen, and a display for displaying an image of at least a portion of the specimen. Furthermore the invention discloses a method for fluorescence microscopy.
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公开(公告)号：DE10142229B4
公开(公告)日：2007-02-01
申请号：DE10142229
申请日：2001-08-29
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN , MOELLMANN KYRA
IPC: G02B21/24
Abstract: A microscope includes a lens holder for receiving a lens. A locking device is provided that affixes the received lens so that the lens cannot be removed without authorization.
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公开(公告)号：DE50009413D1
公开(公告)日：2005-03-10
申请号：DE50009413
申请日：2000-11-07
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN
Abstract: An apparatus for optical scanning of multiple specimens (1) and/or specimen regions, the specimens (1) being associated with a specimen receiving device (2) and being optically scannable by a scanning device (3), is easy to operate for data recording of many specimens in the shortest possible time, using simple and economical optical system components. The apparatus according to the present invention is characterized in that the specimen receiving device (2) is rotatable about an axis (4).
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公开(公告)号：DE10238100A1
公开(公告)日：2004-03-04
申请号：DE10238100
申请日：2002-08-21
Applicant: LEICA MICROSYSTEMS
Inventor： ENGELHARDT JOHANN
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