公开(公告)号：US20150322488A1

公开(公告)日：2015-11-12

申请号：US14730989

申请日：2015-06-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael R. GAMBINI ,  John G. ATWOOD ,  Anthony L. CERRONE ,  Edward J. LAKATOS ,  Eugene F. YOUNG

IPC: C12Q1/68 ,  G01N21/64 ,  B01L7/00

CPC classification number: G01N21/6428 ,  B01L3/5085 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/1811 ,  C12Q1/686 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/06113 ,  G01N2201/0628 ,  G01N2201/0635 ,  G01N2201/0636 ,  G01N2201/068 ,  C12Q2563/107

Abstract: An optical instrument monitors PCR replication of DNA in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded DNA. A beam splitter passes an excitation beam to the vials to fluoresce the dye. An emission beam from the dye is passed by the beam splitter to a CCD detector from which a processor computes DNA concentration. A reference strip with a plurality of reference emitters emit reference beams of different intensity, from which the processor selects an optimum emitter for compensating for drift. Exposure time is automatically adjusted for keeping within optimum dynamic ranges of the CCD and processor. A module of the beam splitter and associated optical filters is associated with selected dye, and is replaceable for different dyes.

Abstract translation: 光学仪器监测具有温度循环阻滞的反应装置中DNA的PCR复制，所述反应装置具有小瓶反应成分，包括在双链DNA存在下发荧光的染料。 分束器将激发光束传递到小瓶以使染料发荧光。 来自染料的发射光束通过分束器传递到CCD检测器，处理器从该CCD检测器计算DNA浓度。 具有多个参考发射器的参考条发射不同强度的参考光束，处理器从该参考光束选择用于补偿漂移的最佳发射器。 自动调整曝光时间以保持CCD和处理器的最佳动态范围。 分束器和相关的滤光器的模块与选定的染料相关联，并且可替代不同的染料。

公开(公告)号：US09052302B2

公开(公告)日：2015-06-09

申请号：US13122561

申请日：2009-09-28

Applicant: Ulrich Lundgreen ,  Aria Farjam ,  Rolf Uthemann ,  Andreas Mitreiter ,  Isabel Huenig ,  Markus Lenhard ,  Rainer Froemel ,  Hans-Joachim Kumpch

Inventor： Ulrich Lundgreen ,  Aria Farjam ,  Rolf Uthemann ,  Andreas Mitreiter ,  Isabel Huenig ,  Markus Lenhard ,  Rainer Froemel ,  Hans-Joachim Kumpch

IPC: G01N33/18 ,  G01N21/31 ,  B01L3/00

CPC classification number: G01N33/1893 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0838 ,  B01L2400/0475 ,  G01N21/3151 ,  G01N21/78 ,  G01N27/4167 ,  G01N27/4168 ,  G01N33/1886 ,  G01N2201/0245

Abstract: A method for determining an analyte in a water sample with a mobile water analyzing system having a basic unit and a test element insertable in the basic unit, the method comprising providing the test element and the basic unit. The test element is inserted into a test element receptacle of the basic unit. The water sample is transported forward from an inlet opening to a measuring section of the test element. A first analyzing of the water sample is performed in the measuring section with an analyzer of the basic unit. The water sample is transported forward from the measuring section to the first reagent section of the test element. The water sample is transported backward from the first reagent section to the measuring section of the test element. A second analyzing of the water sample is performed in the measuring section with the analyzer of the basic unit.

Abstract translation: 一种利用移动式水分析系统确定水样中分析物的方法，所述移动式水分析系统具有可插入基本单元的基本单元和测试元件，该方法包括提供测试元件和基本单元。 将测试元件插入到基本单元的测试元件容器中。 水样从入口开口向前传送到测试元件的测量部分。 在测量部分用基本单元的分析仪进行水样的第一次分析。 水样从测量部分向前传送到测试元件的第一试剂部分。 水样从第一试剂部向测试部的测定部向后方传送。 用基本单位分析仪在测量部分进行水样的第二次分析。

公开(公告)号：US08492138B2

公开(公告)日：2013-07-23

申请号：US13611462

申请日：2012-09-12

Applicant: Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

Inventor： Steven J. Boege ,  Howard G. King ,  Eugene F. Young ,  Johannes P. Sluis ,  Mark F. Oldham

IPC: C12M1/34 ,  C12M3/00

CPC classification number: G01N21/6486 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.

公开(公告)号：US08361785B2

公开(公告)日：2013-01-29

申请号：US12321830

申请日：2009-01-26

Applicant: Mark F. Oldham ,  Eugene F. Young

Inventor： Mark F. Oldham ,  Eugene F. Young

IPC: C12M1/34 ,  C12M3/00 ,  G01N21/64

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L7/52 ,  C12Q2545/101 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2021/6482 ,  G01N2201/0245 ,  G01N2201/0628 ,  G01N2201/0635 ,  Y10S435/808 ,  Y10S435/809

Abstract: An instrument is provided that can monitor nucleic acid sequence amplification reactions, for example, PCR amplification of DNA and DNA fragments. The instrument includes a multi-notch filter disposed along one or both of an excitation beam path and an emission beam path. Methods are also provided for monitoring nucleic acid sequence amplifications using an instrument that includes a multi-notch filter disposed along a beam path.

公开(公告)号：US20120115214A1

公开(公告)日：2012-05-10

申请号：US13245124

申请日：2011-09-26

Applicant: C. Frederick Battrell ,  Troy D. Daiber

Inventor： C. Frederick Battrell ,  Troy D. Daiber

IPC: C12M1/34 ,  G01N21/64

CPC classification number: G01N21/6456 ,  B01L7/52 ,  C12M23/16 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/6454 ,  G01N2201/0245

Abstract: An instrument for fluorometric assays in liquid samples is disclosed. The instrument may include multiple optical channels for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. The disclosed instrument finds utility in any number of applications, including microfluidic molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general.

Abstract translation: 公开了用于液体样品中荧光测定的仪器。 仪器可以包括多个光学通道，用于监测与靶分析物相关联的第一荧光团和与对照相关联的第二荧光团。 所公开的仪器可用于任何数量的应用，包括基于目标核酸的PCR扩增和荧光测定的微流体分子生物学测定。

公开(公告)号：US08130379B1

公开(公告)日：2012-03-06

申请号：US12872207

申请日：2010-08-31

Applicant: Georgiy G. Burba ,  Daniel J. Anderson ,  Michael D. Furtaw ,  Robert D. Eckles ,  Dayle K. McDermitt ,  Jonathan M. Welles

Inventor： Georgiy G. Burba ,  Daniel J. Anderson ,  Michael D. Furtaw ,  Robert D. Eckles ,  Dayle K. McDermitt ,  Jonathan M. Welles

IPC: G01N21/00

CPC classification number: G01N21/3504 ,  G01K13/02 ,  G01K2013/024 ,  G01N1/2247 ,  G01N21/05 ,  G01N2201/0245

Abstract: Gas analyzer systems and methods for determining gas flux in a short intake tube configuration without using any Webb-Pearman-Leuning density correction. Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components.

Abstract translation: 气体分析仪系统和方法，用于确定短进气管配置中的气体通量，而不使用任何Webb-Pearman-Leuning密度校正。 气体分析仪系统和测量气体浓度的方法，特别是气体组分的干摩尔分数。 这些系统和方法允许快速测量多种环境监测应用的气体密度和/或气体的干摩尔分数，包括高速通量测量。 新的耦合设计允许无需移除封闭流动通道的电池，从而可以对光学部件进行现场清洁。

公开(公告)号：US08077309B2

公开(公告)日：2011-12-13

申请号：US11668083

申请日：2007-01-29

Applicant: Gordon C. Brown ,  Michael Burka ,  Brian Cranton ,  David Erickson ,  James Grassi ,  Matthew Patrick Hammond ,  Maximillan Ben Shaffer ,  Pierre Villeneuve

Inventor： Gordon C. Brown ,  Michael Burka ,  Brian Cranton ,  David Erickson ,  James Grassi ,  Matthew Patrick Hammond ,  Maximillan Ben Shaffer ,  Pierre Villeneuve

IPC: G01J3/44

CPC classification number: G01N21/65 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0262 ,  G01J3/0286 ,  G01J3/0291 ,  G01J3/10 ,  G01J3/26 ,  G01J3/36 ,  G01J3/44 ,  G01J2003/1269 ,  G01N2021/1793 ,  G01N2021/8416 ,  G01N2201/0245 ,  G01N2201/067

Abstract: An optical apparatus for measurement of industrial chemical processes. The analyzer uses Raman scattering and performs measurement of chemical concentrations in continuous or batch processes. The analyzer operates at a standoff distance from the analyte (or analytes) and can measure concentrations through an optical port, facilitating continuous, non-destructive, and non-invasive analysis without extracting the analyte or analytes from the process. The analyzer can measure one or several solid, liquid, or gaseous analytes, or a mixture thereof.

Abstract translation: 一种用于测量工业化学工艺的光学装置。 分析仪使用拉曼散射，并在连续或间歇过程中进行化学浓度的测量。 分析仪与分析物（或分析物）隔开距离，并且可以通过光学端口测量浓度，有助于连续，非破坏性和非侵入性分析，而无需从该过程中提取分析物或分析物。 分析仪可以测量一种或多种固体，液体或气体分析物或其混合物。

公开(公告)号：US07892492B2

公开(公告)日：2011-02-22

申请号：US11635312

申请日：2006-12-07

Applicant: Robin H. Bogner ,  Theodore L. Bergman ,  Kristyn Greco ,  Derek J. Michaels ,  Szymon J. Chawarski

Inventor： Robin H. Bogner ,  Theodore L. Bergman ,  Kristyn Greco ,  Derek J. Michaels ,  Szymon J. Chawarski

IPC: G01N15/06 ,  G01N1/10 ,  B01L3/00 ,  B01L99/00 ,  B01L9/00 ,  B01D11/04 ,  B01D11/02

CPC classification number: G01N21/05 ,  G01N21/0317 ,  G01N21/0332 ,  G01N21/09 ,  G01N21/272 ,  G01N2021/0342 ,  G01N2021/056 ,  G01N2201/0245

Abstract: The present disclosure is directed to an apparatus and method for studying dissolution of a compact sample. The compact sample is typically a pharmaceutical drug sample. A flow-through apparatus includes a frame defining a flow-through channel, a removable insert having a drug sample, the insert positioned within the frame such that a fluid interacts with the sample when the fluid passes through the flow channel. The frame has an opening on the top side to allow a glass plate, typically a microscope cover slip to be positioned within the frame and allow viewing of the fluid flow and interaction with the drug sample. The hydrodynamics of the fluid flow are either known or computed. Thus, dissolution can be studied and observed in view of hydrodynamic characteristics. Typically, only a small amount of sample is necessary for a study. The flow-through apparatus is designed to fit on a microscopy stage and allow visual observation of the fluid/sample interaction.

Abstract translation: 本公开涉及用于研究紧凑样品的溶解的装置和方法。 紧凑型样品通常是药物药物样品。 流通装置包括限定流通通道的框架，具有药物样本的可移除插入件，插入件定位在框架内，使得流体在流体通过流动通道时与样品相互作用。 框架在顶侧具有开口，以允许玻璃板，通常是显微镜盖滑动件定位在框架内，并允许观察流体流动和与药物样品的相互作用。 流体流动的流体动力学是已知的或计算的。 因此，考虑到流体动力特性，可以研究和观察溶解。 通常，研究只需要少量样品。 流通装置设计成适合于显微镜台上，并允许目视观察流体/样品相互作用。

公开(公告)号：US20100110437A1

公开(公告)日：2010-05-06

申请号：US12266379

申请日：2008-11-06

Applicant: Michael D. Furtaw ,  Robert D. Eckles ,  Georgiy G. Burba ,  Dayle K. McDermitt ,  Jonathan M. Welles

Inventor： Michael D. Furtaw ,  Robert D. Eckles ,  Georgiy G. Burba ,  Dayle K. McDermitt ,  Jonathan M. Welles

IPC: G01N21/00 ,  G01N7/00 ,  G01K13/00

CPC classification number: G01N21/3504 ,  G01K13/02 ,  G01K2013/024 ,  G01N1/2247 ,  G01N21/05 ,  G01N2201/0245

Abstract: Gas analyzer systems and methods for measuring concentrations of gasses and in particular dry mole fraction of components of a gas. The systems and method allow for rapid measurement of the gas density and/or dry mole fraction of gases for a number of environmental monitoring applications, including high speed flux measurements. A novel coupling design allows for tool-free removal of a cell enclosing a flow path to enable in field cleaning of optical components.

Abstract translation: 气体分析仪系统和测量气体浓度的方法，特别是气体组分的干摩尔分数。 这些系统和方法允许快速测量多种环境监测应用的气体密度和/或气体的干摩尔分数，包括高速通量测量。 新的耦合设计允许无需移除封闭流动通道的电池，从而可以对光学部件进行现场清洁。

公开(公告)号：US07645424B2

公开(公告)日：2010-01-12

申请号：US11434211

申请日：2006-05-16

Applicant: Michael O'Donovan

Inventor： Michael O'Donovan

IPC: B01L3/00 ,  B01L3/02 ,  C12M1/34 ,  C12M3/00 ,  C12M3/04 ,  C12M1/24

CPC classification number: B01L3/50825 ,  B01L3/502 ,  B01L3/5082 ,  B01L3/5635 ,  B01L2200/026 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2300/0672 ,  B01L2400/0683 ,  G01N21/03 ,  G01N2021/0321 ,  G01N2021/0325 ,  G01N2021/0364 ,  G01N2201/0245

Abstract: A reagent cuvette has a first chamber with an inspection part and a socket, and a second chamber. The socket has four spikes at its base. Both chambers are sealed with a membrane. At the point-of-care the foil membrane of the first chamber is peeled away by the therapist (typically general practitioner doctor). A sample, such as blood, is added to the chamber using a pipette or other device to provide a verifiable quantity of sample. This provides a mixture of a buffer reagent supplied in the chamber and the sample injected into the inspection chamber at the point of care. The chamber is then inserted into the socket by gently pressing it down so that its foil membrane is broken by the spikes. This causes the starter reagent to drop down from within the second chamber into the inspection part of the first chamber. The inspection part is then inserted into an optical inspection instrument for analysis of the two reagents and the sample mixed together.

Abstract translation: 试剂池具有具有检查部分和插座的第一室和第二室。 插座在其底部有四个尖峰。 两个室都用膜密封。 在护理点，第一室的箔膜由治疗师（通常是全科医生）剥离。 使用移液管或其他装置将诸如血液的样品加入到腔室中以提供可验证量的样品。 这提供了在室内提供的缓冲试剂和在注意点处注入检查室的样品的混合物。 然后通过轻轻地将其插入插座中，使其箔片被尖钉破坏。 这使起动剂试剂从第二室内下降到第一室的检查部分。 然后将检查部件插入光学检查仪器中，分析两种试剂和样品混合在一起。