-
公开(公告)号：KR1020140055563A
公开(公告)日：2014-05-09
申请号：KR1020120122610
申请日：2012-10-31
Applicant: 고려대학교 산학협력단
Abstract: The present invention provides a magnetic core/shell nanoparticle comprising: a) a core part that is formed as magnetic material and boron are bonded; b) a silica shell part that wraps the core; c) an outermost shell part that warps the shell and includes silica; d) an outermost shell surface part that is obtained by improving the surface of the outermost shell through one or more substituents selected from the group consisting of -NH_2, -COOH, -NHS, and -biotin; and e) an enzyme or a biomaterial that is fixed to the shell surface part. The core/shell nanoparticle including the immobilized enzyme or biomaterial according to the present invention has the surface that is improved by means of amine groups and the like. The immobilization is optimized by means of a covalent bond of the enzyme or biomaterial and a carboxyl group. Even though the nanoparticle is used several times, the activity of the enzyme or biomaterial can be maintained at a high level.
Abstract translation: 本发明提供一种磁芯/壳纳米颗粒，其包括：a）形成为磁性材料和硼键合的芯部; b）包裹芯的二氧化硅壳部分; c）最外壳部分，其使壳体翘曲并包括二氧化硅; d）通过一种或多种选自-NH 2，-COOH，-NHS和 - 生物素的取代基改进最外壳的表面而获得的最外壳表面部分; 和e）固定到壳表面部分的酶或生物材料。 包含根据本发明的固定化酶或生物材料的核/壳纳米颗粒具有通过胺基等改进的表面。 通过酶或生物材料的共价键和羧基来优化固定。 即使使用纳米颗粒多次，酶或生物材料的活性也可以维持在高水平。
-
公开(公告)号：KR101363293B1
公开(公告)日：2014-02-19
申请号：KR1020120022609
申请日：2012-03-06
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 락툴로오스 합성용 마이크로 반응기 및 락툴로오스 생산 방법에 관한 것으로, 구체적으로 마이크로 반응기에 고정화된 베타-갈락토시다제에 의하여 유청 및 과당을 포함하는 기질과 반응시켜 경제적으로 프리바이오틱스의 하나인 락툴로오스의 생산이 가능하며, 생물학적인 연속 생산이 가능한 마이크로 반응기 및 락툴로오스 생산 방법에 관한 것이다.
-
53.发明公开
公开(公告)号：KR1020130055309A
公开(公告)日：2013-05-28
申请号：KR1020110120991
申请日：2011-11-18
Applicant: 고려대학교 산학협력단
CPC classification number: C01G33/00 , B82Y40/00 , C01P2004/16
Abstract: PURPOSE: A manufacturing method of a KNbO3 nanowire is provided to obtain the KNbO3 with high yield, in short time by using an Nb metal wire. CONSTITUTION: A manufacturing method of a KNbO3 nanowire comprises a step of conduct reaction of mixed solution of niobium metal powder and potassium hydroxide aqueous solution at 130-170 °C for 6-36 hours, to obtain the KNbO3 nanowire. The manufacturing method of the KNbO3 nanowire additionally includes a step of injecting the KNbO3 nanowire into distilled water, centrifugating, washing, and drying the same. The washing is repeated until the time the pH of the nanowire reaches to 7. The manufacturing method of the KNb03 nanowire additionally includes a step of heat treating the nanowire at 250-460 °C for 0.5-1 hours, to convert the crystal phase of the nanowire into orthorhombic system. The thickness of the Knb03 nanowire has a thickness of 60-90 nm and 3.5-6.5 Mm. The crystal phase of the nanowire at room temperature is a monoclinic system.
Abstract translation: 目的：提供KNbO3纳米线的制造方法，通过使用Nb金属线在短时间内以高产率获得KNbO 3。 构成：KNbO 3纳米线的制造方法包括将铌金属粉末和氢氧化钾水溶液的混合溶液在130-170℃下进行6-36小时的步骤，得到KNbO 3纳米线。 KNbO3纳米线的制造方法还包括将KNbO 3纳米线注射到蒸馏水中，离心，洗涤和干燥的步骤。 重复洗涤直到纳米线的pH达到7为止.KNbO 3纳米线的制造方法还包括在250-460℃下对纳米线进行0.5-1小时的热处理以将晶体相转变为 纳米线成斜方晶系。 Knb03纳米线的厚度为60-90nm，厚度为3.5-6.5μm。 纳米线在室温下的结晶相是单斜晶系。
-
公开(公告)号：KR101193943B1
公开(公告)日：2012-10-24
申请号：KR1020100064542
申请日：2010-07-05
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 셀로비오스 디하이드로게나제를 공유결합(covalent binding)을 이용하여 담체에 고정화시키기 전에 전처리하는 방법에 관한 것이다. 구체적으로 셀로비오스 디하이드로게나제를 담체에 고정화시키기 전에 셀로비오스 디하이드로게나제 용액에 단당류, 이당류, 다당류 또는 이들의 혼합물을 첨가하여 반응시키는 전처리 과정을 통하여 공유결합에 의한 셀로비오스 디하이드로게나제의 고정화 시 발생하는 활성도의 감소를 억제하고 여러 번 재사용할 경우에도 셀로비오스 디하이드로게나제의 활성을 유지할 수 있다.
-
公开(公告)号：KR1020120098240A
公开(公告)日：2012-09-05
申请号：KR1020110018070
申请日：2011-02-28
Applicant: 고려대학교 산학협력단
CPC classification number: C12P19/02 , C12N9/2491 , C12P19/14 , C12Y302/01025
Abstract: PURPOSE: A mannanase isolated from Clostridium cellulovorans is provided to ensure excellent enzyme activation and to effectively produce biofuel from ligneous biomass. CONSTITUTION: A mannanase isolated from Clostridium cellulovorans contains has an amino acid sequence of sequence number 1. A gene encoding the mannanase has a base of sequence number 2. A recombinant vector contains the gene. A method for decomposing ligneous biomass comprises a step of treating the ligneous biomass with the mannanase. A composition for decomposing ligneous biomass contains a complex of mini CbpA and endoglucanase E as an active ingredient.
Abstract translation: 目的：提供从Clostridium cellulovorans分离的甘露聚糖酶，以确保优异的酶活化和有效生产木质生物质的生物燃料。 构成：从Clostridium cellulovorans分离的甘露聚糖酶含有序列号1的氨基酸序列。编码甘露聚糖酶的基因具有序列号2的碱基。重组载体含有该基因。 用于分解木质生物质的方法包括用甘露聚糖酶处理木质生物量的步骤。 用于分解木质生物质的组合物含有迷你CbpA和内切葡聚糖酶E作为活性成分的复合物。
-
Patent Agency Ranking
公开(公告)号：KR1020120069275A
公开(公告)日：2012-06-28
申请号：KR1020100130759
申请日：2010-12-20
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: An immobilizing method for glucose isomerase through pretreatment is provided to maintain enzyme activity. CONSTITUTION: An immobilizing method for glucose isomerase by covalent bond comprises a step of reacting the glucose isomerase with monosaccharides before immobilization. The monosaccharides are pentose or hexose. The concentration of the monosaccharide is 0.5-3.0 M. The immorbilization is performed at 15°C-25°C for 6-30 hours.
Abstract translation: 目的：提供通过预处理的葡萄糖异构酶的固定方法，以维持酶活性。 构成：通过共价键的葡萄糖异构酶的固定方法包括在固定前使葡萄糖异构酶与单糖反应的步骤。 单糖是戊糖或己糖。 单糖的浓度为0.5-3.0M。脱敏在15℃-25℃下进行6-30小时。
-
公开(公告)号：KR101097370B1
公开(公告)日：2011-12-23
申请号：KR1020090048100
申请日：2009-06-01
Applicant: 고려대학교 산학협력단
CPC classification number: H01M8/16 , Y02E60/527 , Y02P70/56
Abstract: 본발명에따르면, DNA와탄소나노튜브를이용한전극을적용하여효소연료전지를제작할때 전극에고정화되는효소의활성부위를보호하고연료의산화반응효소를효과적으로애노드에고정화하는방법을제공한다. 이러한방법으로제작된효소연료전지는기존의효소고정화기술이적용된연료전지의효소활성및 안정성을높여향상된파워생산량을나타내게된다.
-
公开(公告)号：KR1020110076217A
公开(公告)日：2011-07-06
申请号：KR1020090132866
申请日：2009-12-29
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for culturing microorganism by mixed culture is provided to improve microorganism growth and metabolic product productivity. CONSTITUTION: A method for culturing microorganism comprises a step of mixing and culturing a first microorganism and a second microorganism. The second microorganism has a cellular activity-remove/attenuated microorganism. The first microorganism is Acremonium sp. The second microorganism is E.coli, Ankistrodesmus sp., S. hygroscopicus subsp. The first microorganism is pre-cultured before mixing the second microorganism.
Abstract translation: 目的：提供通过混合培养培养微生物的方法，以改善微生物生长和代谢产物生产率。 构成：培养微生物的方法包括混合培养第一微生物和第二微生物的步骤。 第二微生物具有细胞活性去除/减毒的微生物。 第一种微生物是顶孢菌属 第二种微生物是大肠杆菌，Ankistrodesmus sp。，吸湿链霉菌subsp。 第一微生物在混合第二微生物之前进行预培养。
-
公开(公告)号：KR1020110076165A
公开(公告)日：2011-07-06
申请号：KR1020090132798
申请日：2009-12-29
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for culturing microorganisms is provided to promoted growth or differentiation of microorganisms and to improve productivity of metabolites. CONSTITUTION: A method for culturing microorganism comprises a step of adding solid materials having 0.9-2.5 specific gravity. The solid material is a silicon. The solid material is a spherical, cylindrical, cone, trapezoid cylindrical, or hexahedral shape. The average diameter of the solid material is 0.1-100 mm. The microorganism is mycelium microorganism. The mycelium microorganism is Acremonium chrysogenum. The microorganism is cultured by shaking or stirring at 150-500rpm and 20-35°C for 48-240 hours.
Abstract translation: 目的：提供培养微生物的方法，以促进微生物的生长或分化，提高代谢产物的生产力。 构成：培养微生物的方法包括添加具有0.9-2.5比重的固体材料的步骤。 固体材料是硅。 固体材料是球形，圆柱形，锥形，梯形圆柱形或六面体形状。 固体材料的平均直径为0.1-100mm。 微生物是菌丝体微生物。 菌丝体微生物是产黄绿葡萄孢。 通过在150-500rpm和20-35℃下摇动或搅拌培养微生物48-240小时。
-
公开(公告)号：KR1020100062167A
公开(公告)日：2010-06-10
申请号：KR1020080120624
申请日：2008-12-01
Applicant: 고려대학교 산학협력단
CPC classification number: Y02E60/527 , Y02P70/56
Abstract: PURPOSE: Buffer compositions for a cathode electrolyte of enzymatic fuel cells are provided to enhance enzyme activity and stability of the enzymatic fuel cell, to make a suitable electron transport environment, and to increase a voltage, a current, and a power density. CONSTITUTION: Buffer compositions for a cathode electrolyte of enzymatic fuel cells include a complex phosphate-MOPS buffer. The complex phosphate-MOPS buffer is manufactured by mixing the phosphate buffer and the MOPS buffer with a weight ratio of 4:6 ~ 6:4, respectively. The complex phosphate-MOPS buffer has Ph of 6.5-7.5. The cathode electrolyte of the enzymatic fuel cell includes hydrogen peroxide(H_2O_2) and ABTS(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt).
Abstract translation: 目的：提供酶燃料电池的阴极电解质的缓冲组合物，以提高酶燃料电池的酶活性和稳定性，从而形成合适的电子传输环境，并提高电压，电流和功率密度。 构成：用于酶燃料电池的阴极电解质的缓冲剂组合物包括复合磷酸盐-MPS缓冲剂。 通过以4:6〜6:4的重量比混合磷酸盐缓冲液和MOPS缓冲液来制备复合磷酸盐-MPS缓冲液。 复合磷酸盐-MPS缓冲液的Ph为6.5-7.5。 酶燃料电池的阴极电解质包括过氧化氢（H_2O_2）和ABTS（2,2'-氮杂双（3-乙基苯并噻唑啉-6-磺酸）二铵盐）。
-
-
-
-
-
-
-
-
-
Search Results
106
records
(took 0.029 seconds)
