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公开(公告)号：KR1020130101690A
公开(公告)日：2013-09-16
申请号：KR1020120022610
申请日：2012-03-06
Applicant: 고려대학교 산학협력단
IPC: C12P19/12 , C12N9/38 , A23L1/30 , A61K31/7016
CPC classification number: C12P19/12 , C12Y302/01023
Abstract: PURPOSE: A method for producing lactulose is provided to improve economic efficiency of a process, to recycle resources by utilizing a lactose-containing waste liquid, and to produce prebiotics. CONSTITUTION: A method for producing lactulose comprises the steps of: immobilizing beta-galactosidase on a carrier; filling the immobilized carrier in a packed bed reactor; and injecting a substrate containing whey and fructose to the packed bed reactor and inducing a reaction. A system for producing lactulose comprises: the packed bed reactor (11) filled with carrier-immobilized beta-galactosidase; a substrate injection unit for injecting the substrate containing whey and fructose into the packed bed reactor; and a temperature control unit which controls reaction temperature of the packed bed reactor.
Abstract translation: 目的：提供一种生产乳果糖的方法，以提高工艺的经济效率，利用含乳糖的废液回收资源，并产生益生元。 构成：生产乳果糖的方法包括以下步骤：将β-半乳糖苷酶固定在载体上; 在填充床反应器中填充固定化载体; 并将含有乳清和果糖的底物注射到填充床反应器中并诱导反应。 用于制备乳果糖的系统包括：填充有载体固定的β-半乳糖苷酶的填充床反应器（11） 用于将含有乳清和果糖的基质注入到填充床反应器中的基板注入单元; 以及控制填充床反应器的反应温度的温度控制单元。
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公开(公告)号：KR101231120B1
公开(公告)日：2013-02-07
申请号：KR1020100130759
申请日：2010-12-20
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 전처리를 통한 글루코스 이성화효소의 고정화 방법에 관한 것으로서, 보다 구체적으로는 글루코스 이성화효소를 단당류와 반응시키는 전처리 과정을 거침으로써 글루코스 이성화효소의 활성부위를 보호한 뒤 담체에 고정화 시키는 고정화 방법에 관한 것이다.
본 발명에 따라 전처리된 글루코스 이성화효소를 담체에 고정화하였을 경우, 고정화 후에도 높은 활성도를 유지할 수 있으며, 재사용시에도 활성도 감소가 적은 글루코스 이성화효소의 고정화 방법을 제공할 수 있다.-
公开(公告)号：KR1020120065878A
公开(公告)日：2012-06-21
申请号：KR1020100127213
申请日：2010-12-13
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A method for beta-glucosidase fixation through pre-treatment is provided to maintain high activity of the enzyme in case of fixing the enzyme on a carrier. CONSTITUTION: A method for beta-gludosidase fixation comprises a step of fixing the beta-glucosidase on a carrier and a step of pre-treating the beta-glucosidase with saccharides at 30°C-70°C and 50-200 rpm for 5-60 minutes. The saccharides include monosaccharides, disaccharides, or mixture thereof. The monosaccharides and disaccharides are glucose and cellobiose, respectively. The carrier is a silica carrier.
Abstract translation: 目的：提供通过预处理的β-葡糖苷酶固定方法，以在将酶固定在载体上的情况下保持酶的高活性。 构成：β-葡萄糖苷酶固定的方法包括将β-葡糖苷酶固定在载体上的步骤，以及在30℃-70℃和50-200rpm下用糖预处理β-葡糖苷酶的步骤， 60分钟。 糖类包括单糖，二糖或其混合物。 单糖和二糖分别是葡萄糖和纤维二糖。 载体是二氧化硅载体。
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公开(公告)号：KR101800469B1
公开(公告)日：2017-11-24
申请号：KR1020120135853
申请日：2012-11-28
Applicant: 고려대학교 산학협력단
Abstract: 본발명은, (a) 활성탄에산, 산화제또는그 혼합물을처리하여활성탄의표면을산화하는단계; (b) 상기표면이산화된활성탄을, -NH, -COOH, -NHS 및바이오틴(-Biotin)으로이루어진군부터선택되는치환기로개질하는단계; 및 (c) 상기개질된활성탄에, 리파아제(lipase), 글루코오즈옥시데이즈, 라카아제(laccase), 카탈라아제(catalase), 미생물, 항원및 항체로이루어진군으로부터선택되는하나이상의효소또는바이오물질을고정화하는단계를포함하는, 활성탄에효소또는바이오물질을고정화하는방법을제공한다. 본발명에따라활성탄을개질하여효소등을고정화하는경우, 고활성의효소촉매생산공정의개발이가능하여, 환경및 에너지문제에많은기여를할 것으로기대된다.
Abstract translation: （A）通过用酸，氧化剂或其混合物处理活性炭来氧化活性炭的表面; （b）使表面氧化的活性炭与-NH反应
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公开(公告)号：KR1020140094035A
公开(公告)日：2014-07-30
申请号：KR1020120144410
申请日：2012-12-12
Applicant: 고려대학교 산학협력단
IPC: G01N33/53 , C12Q1/68 , G01N33/551 , G01N33/52
CPC classification number: G01N33/5308 , C12Q1/6834 , C12Q2563/107 , C12Q2563/143 , G01N33/551
Abstract: The present invention relates to DNA labeled fluorescent magnetic core/shell nanoparticles with modified particle surfaces and a quantitative analysis method using the same by fluorescence and DNA detection, wherein particle surfaces are modified for utilizing properties and functions of fluorescent magnetic particles in a detection system through nanobio sensors, or the like, and DNA is grafted on the particles to be applied to an antigen-antibody reaction. The detection method of bioactive materials of the present invention uses fluorescent magnetic core/shell nanoparticles to enable detection and quantitative analysis of samples present in low concentrations in a short period of time, thereby reducing time and costs for analyzing bioactive materials such as pesticide residue in processed food, agricultural product, etc.
Abstract translation: 本发明涉及具有改性粒子表面的DNA标记的荧光磁性核/壳纳米颗粒和通过荧光和DNA检测使用其的定量分析方法，其中改变颗粒表面以利用检测系统中荧光磁性颗粒的性质和功能 纳米生物传感器等，并将DNA接枝到待应用于抗原 - 抗体反应的颗粒上。 本发明的生物活性物质的检测方法使用荧光磁芯/壳纳米粒子，能够在短时间内对低浓度的样品进行检测和定量分析，从而减少分析生物活性物质如农药残留的时间和成本 加工食品，农产品等
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Patent Agency Ranking
公开(公告)号：KR1020140068415A
公开(公告)日：2014-06-09
申请号：KR1020120135853
申请日：2012-11-28
Applicant: 고려대학교 산학협력단
Abstract: The present invention provides a method for immobilizing enzyme or biomaterial on active carbon which comprises the steps of: oxidizing surface of active carbon by processing acid, an oxidizing agent, or a mixture thereof with the active carbon; (b) reforming the active carbon in which the surface is oxidized with a substituent selected from a group including -NH2, -COOH, -NHS and -Biotin; and (c) immobilizing one or more enzymes or biomaterials selected from a group including lipase, glucose oxydase, laccase, catalase, microorganism, antigen, and antibody on the reformed active carbon. By immobilizing enzymes and the likes by reforming the active carbon according to the present invention, development of production progress for highly active enzyme catalysts can be possible, thereby high contribution to environmental and energy problems can be made.
Abstract translation: 本发明提供了一种将酶或生物材料固定在活性炭上的方法，包括以下步骤：通过加工酸，氧化剂或其与活性炭的混合物来氧化活性炭的表面; （b）用选自包括-NH 2，-COOH，-NHS和生物素的取代基重整表面被氧化的活性炭; 和（c）将一种或多种选自脂肪酶，葡萄糖氧化酶，漆酶，过氧化氢酶，微生物，抗原和抗体的酶或生物材料固定在重整的活性炭上。 通过根据本发明固化酶等来改变活性炭，可以开发高活性酶催化剂的生产进程，从而可以对环境和能源问题做出高度的贡献。
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公开(公告)号：KR101338524B1
公开(公告)日：2013-12-10
申请号：KR1020120021127
申请日：2012-02-29
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 면 부스러기로부터 바이오연료를 제조하는 방법에 관한 것으로, 더욱 상세하게는 기름에 튀겨진 면 생산 공정 시 생기는 부스러기의 음식물 쓰레기로의 처분이 아닌 바이오연료로 전환시키기 위한 것이다.
즉, 본 발명은 면에 남아있는 오일을 추출하여 화학적, 효소적 공정을 이용하여 바이오디젤로 생산함과 동시에 오일을 제거한 면을 사용하여 당화, 발효 공정을 거쳐 바이오에탄올을 생산한다.
따라서, 본 발명은 일반 공장에서 폐기 처분하는 면을 이용하여 현재 많이 대두되고 있는 바이오연료를 생성하므로 경제적으로나 환경적으로 뛰어난 효과가 있다.-
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公开(公告)号：KR101745079B1
公开(公告)日：2017-06-09
申请号：KR1020150053256
申请日：2015-04-15
Applicant: 고려대학교 산학협력단
Abstract: 본발명은 2,3-부탄다이올을생산하는균주가접종된배지에금속아세테이트또는암모늄아세테이트를첨가하는단계를포함하는 2,3-부탄다이올생산방법에관한것이다. 본발명의생산방법은금속아세테이트또는암모늄아세테이트를균주에첨가함에따라상기균주의생육조건에영향을미치지않으면서 2,3-부탄다이올의생산량과생산성을향상시키는것이특징이며, 이에따라지속적인 2,3-부탄다이올의생산이가능하다.
Abstract translation: 本发明涉及生产2,3-丁二醇的方法，该方法包括向接种有产生2,3-丁二醇的菌株的培养基中加入金属乙酸盐或乙酸铵的步骤。 本发明的制造方法的特征在于，向菌株中添加金属乙酸盐或乙酸铵可提高2,3-丁二醇的收率和生产率，而不影响菌株的生长条件， 可以生产3-丁二醇。
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公开(公告)号：KR1020140055566A
公开(公告)日：2014-05-09
申请号：KR1020120122616
申请日：2012-10-31
Applicant: 고려대학교 산학협력단
CPC classification number: B82B1/008 , B01J13/025 , B82B3/0095 , B82Y40/00 , C12N9/00
Abstract: The present invention comprises: (a) a core unit formed by a combination of a magnetic substance and boron; (b) a silica shell unit embracing the core; (c) a surface part of an ultimate outskirt shell where the surface of the ultimate outskirt shell is reformed to a substituent selected from the groups of -NH2, -COOH, -NHS and -Biotin; and a magnetic core/shell nanoparticle consisting of an enzyme or a biomaterial fixated onto the surface part of the shell, and encapsulated by chitosan. According to the present invention, the encapsulated magnetic core/shell nanoparticle maintains the vitality of the enzyme or the biomaterial at a high standard even when re-used a number of times, and the structure of the enzyme or the biomaterial is stably protected.
Abstract translation: 本发明包括：（a）由磁性物质和硼的组合形成的核心单元; （b）包围该芯的二氧化硅壳单元; （c）终极外壳的表面部分，其中最终外裙壳的表面被重整成选自-NH 2，-COOH，-NHS和生物素的取代基; 以及由酶或生物材料组成的磁性核/壳纳米颗粒固定在壳的表面部分上并被壳聚糖包封。 根据本发明，包封的磁性核/壳纳米颗粒即使重复使用多次也能保持酶或生物材料的活力，甚至可以稳定地保护酶或生物材料的结构。
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