公开(公告)号：KR1020090114897A

公开(公告)日：2009-11-04

申请号：KR1020080040773

申请日：2008-04-30

Applicant: 포항공과대학교 산학협력단

Inventor： 정규열 ,  박진현 ,  조양숙 ,  신기원 ,  구정모

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/686

Abstract: PURPOSE: A method for modifying a template for analysis multiple mRNA or 16S rRNA is provided to detect microorganism by shortening analysis time and enhance analysis sensitivity and accuracy. CONSTITUTION: A method for modifying a template for analysis of multiple mRNA or 16S rRNA comprises: a step of performing reverse transcriptase PCR with a primer containing template-specific sequence and overhang sequence of mRNA or 18S rRNA to prepare cDNA having universal base sequence; a step of constructing template switching primer; and a step of performing PCR with the primer to obtain double strand DNA template with common sequence at both terminal of the sequence.

Abstract translation: 目的：通过缩短分析时间，提高分析灵敏度和准确度，提供了一种修改模板以分析多个mRNA或16S rRNA的方法。 构成：修饰用于分析多个mRNA或16S rRNA的模板的方法包括：用含有模板特异性序列和mRNA或18S rRNA突出序列的引物进行逆转录酶PCR以制备具有通用碱基序列的cDNA的步骤; 构建模板切换引物的一个步骤; 以及用引物进行PCR的步骤，以获得序列两端的共同序列的双链DNA模板。