公开(公告)号：KR101606929B1

公开(公告)日：2016-03-29

申请号：KR1020080040773

申请日：2008-04-30

Applicant: 포항공과대학교 산학협력단

Inventor： 정규열 ,  박진현 ,  조양숙 ,  신기원 ,  구정모

IPC: C12Q1/68

Abstract: 본발명은모세관전기영동에의한다중 mRNA 또는 16S rRNA 분석을위한주형변형방법및 이를이용한미생물검출방법에관한것으로, 보다상세하게는분석대상인 mRNA 또는 rRNA를동시에여러종 증폭하기위하여주형이되는서열의앞뒤에공통의염기서열을갖는변형된이중가닥 DNA 주형을제작하고, 공통프라이머를이용하여변형된이중가닥 DNA 주형을 PCR 증폭한다음, 증폭된산물을모세관전기영동-단일쇄형태변환다형성(single strand conformation polymorphism)을통해분리및 정량함으로써분석민감도및 정확도가높고분석시간이크게단축되는효과를가짐과동시에이를이용하여미생물을검출할수 있는모세관전기영동에의한다중 mRNA 또는 16S rRNA 분석을위한주형변형방법및 이를이용한미생물검출방법에관한것이다.

公开(公告)号：KR1020090114897A

公开(公告)日：2009-11-04

申请号：KR1020080040773

申请日：2008-04-30

Applicant: 포항공과대학교 산학협력단

Inventor： 정규열 ,  박진현 ,  조양숙 ,  신기원 ,  구정모

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/686

Abstract: PURPOSE: A method for modifying a template for analysis multiple mRNA or 16S rRNA is provided to detect microorganism by shortening analysis time and enhance analysis sensitivity and accuracy. CONSTITUTION: A method for modifying a template for analysis of multiple mRNA or 16S rRNA comprises: a step of performing reverse transcriptase PCR with a primer containing template-specific sequence and overhang sequence of mRNA or 18S rRNA to prepare cDNA having universal base sequence; a step of constructing template switching primer; and a step of performing PCR with the primer to obtain double strand DNA template with common sequence at both terminal of the sequence.

Abstract translation: 目的：通过缩短分析时间，提高分析灵敏度和准确度，提供了一种修改模板以分析多个mRNA或16S rRNA的方法。 构成：修饰用于分析多个mRNA或16S rRNA的模板的方法包括：用含有模板特异性序列和mRNA或18S rRNA突出序列的引物进行逆转录酶PCR以制备具有通用碱基序列的cDNA的步骤; 构建模板切换引物的一个步骤; 以及用引物进行PCR的步骤，以获得序列两端的共同序列的双链DNA模板。

公开(公告)号：KR1020080111353A

公开(公告)日：2008-12-23

申请号：KR1020070059649

申请日：2007-06-18

Applicant: 포항공과대학교 산학협력단

Inventor： 정규열 ,  박영섭 ,  박진현 ,  조양숙 ,  신기원 ,  구정모

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2537/143 ,  C12Q2565/125

Abstract: A method for detecting microorganism and an apparatus performing the same are provided to analyze the kind and amount of the microorganism included within a sample by omitting a step for cultivating the microorganism in detection. A method for detecting microorganism comprises the first step for amplifying the target gene by performing the multi-PCR having the genomic DNA separated from sample as the template DNA; and the second step for analyzing the kind and amount of the microorganism included within a sample by performing the single strand conformational polymorphism for the amplified target gene.

Abstract translation: 提供了检测微生物的方法和进行该微生物的装置，以通过省略在检测中培养微生物的步骤来分析样品中包含的微生物的种类和数量。 检测微生物的方法包括通过进行具有从样品分离出的基因组DNA作为模板DNA的多重PCR扩增靶基因的第一步骤; 以及通过对扩增的靶基因进行单链构象多态性来分析样品中包含的微生物的种类和量的第二步骤。