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公开(公告)号:KR1019890004023B1
公开(公告)日:1989-10-16
申请号:KR1019870014288
申请日:1987-12-15
Applicant: 한국과학기술연구원
IPC: C12N1/38
Abstract: The method for preparing Rhizobium as inoculum comprises: (a) culturing Rhizobium japonium KCTC in the medium containing 100g of mannitol, 1.0 of yeast extract, 0.2 of MgSO4, 0.2 of NaCl, 0.2 of K2HPO4 and 1 liter distilled water at 28≦̸C for 4 days; (b) pulverizing 100g of perlite and sterilizing at 121 C for 2 hrs; (c) putting the pulverized perlite to the above culture broth in an aseptic condition and mixing; (d) aging the mixture at 25≦̸C for two weeks.
Abstract translation: 用于制备根瘤菌作为接种物的方法包括:(a)在含有100g甘露醇,1.0份酵母提取物,0.2份MgSO 4,0.2份NaCl,0.2份K 2 HPO 4和2升蒸馏水的培养基中培养根瘤菌KCTC,在28& 4天; (b)粉碎100g珍珠岩并在121℃下灭菌2小时; (c)在无菌条件下将粉碎的珍珠岩放入上述培养液中并混合; (d)将混合物在25℃下老化2周。
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