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51.发明公开트랜스포존과 Cre/loxP 부위 특이적 재조합 방법을 이용하는 염색체의 특정부위가 제거된 미생물 변이주 제조방법 失效使用TRANSPOSON和CRE / LOXP位点特异性重组来除去特异性染色体中的微生物突变菌株的方法
公开(公告)号：KR1020030069708A
公开(公告)日：2003-08-27
申请号：KR1020020009647
申请日：2002-02-22
Applicant: 한국과학기술원
IPC: C12N15/63
Abstract: PURPOSE: A method for preparing microorganism mutant strains in which a specific chromosome is removed using transposon and Cre/loxP site-specific recombination is provided, thereby effectively removing specific portions of chromosome from microorganisms. CONSTITUTION: A transposon TnKGloxP comprises a transposase recognition site having the nucleotide sequence of SEQ ID NO: 3 in one end, another transposase recognition site having the nucleotide sequence complementary with the SEQ ID NO: 3 in another end, loxp region having the nucleotide sequence of SEQ ID NO: 4, KmR gene having the nucleotide sequence of SEQ ID NO: 5 and GFP gene having the nucleotide sequence of SEQ ID NO: 6. A transposon TnCloxP comprises a transposase recognition site having the nucleotide sequence of SEQ ID NO: 3 in one end, another transposase recognition site having the nucleotide sequence complementary with the SEQ ID NO: 3 in another end, loxP region having the nucleotide sequence of SEQ ID NO: 4 and CmR gene having the nucleotide sequence of SEQ ID NO: 7. A method for preparing microorganism mutant strains in which a specific chromosome is removed using transposon and Cre/loxP site-specific recombination comprises the steps of: preparing two transposons having different selection markers; inserting two transposons into each chromosome of each microorganism; positioning transposons on a chromosome using P1 phage transduction; and inserting a Cre gene into the chromosome, and expressing the Cre gene to a portion of chromosome between two loxP regions.
Abstract translation: 目的：提供使用转座子和Cre / loxP位点特异性重组来除去特异性染色体的微生物突变菌株的方法，从而有效地从微生物中除去染色体的特定部分。 构成：转座子TnKGloxP包含在一端具有SEQ ID NO：3的核苷酸序列的转座酶识别位点，另一端具有与SEQ ID NO：3互补的核苷酸序列的另一转座识别位点，具有核苷酸序列的loxp区 具有SEQ ID NO：5的核苷酸序列的KmR基因和具有SEQ ID NO：6的核苷酸序列的GFP基因。转座子TnCloxP包含具有SEQ ID NO：4的核苷酸序列的转座子识别位点。 在另一端具有与SEQ ID NO：3互补的核苷酸序列的另一个转座酶识别位点，具有SEQ ID NO：4的核苷酸序列的loxP区域和具有SEQ ID NO：7的核苷酸序列的CmR基因 制备使用转座子和Cre / loxP位点特异性重组除去特异性染色体的微生物突变菌株的方法包括以下步骤：制备两个转座子havi 不同的选择标记; 将两个转座子插入每个微生物的每个染色体中; 使用P1噬菌体转导在染色体上定位转座子; 并将Cre基因插入染色体，并将Cre基因表达到两个loxP区域之间的一部分染色体。
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公开(公告)号：KR100330136B1
公开(公告)日：2002-03-27
申请号：KR1019990005078
申请日：1999-02-12
Applicant: 한국과학기술원
IPC: A61K38/10
Abstract: PURPOSE: Provided are novel antibacterial peptide parasin I separated from parasilurus asotus, showing strong antibacterial activity and the use thereof for treatment of wound, cleansing of the oral cavity. CONSTITUTION: A novel antibacterial peptide has the amino acid sequence of KGRGKGGKVRAKAKTRSS (I). The amino acid sequence is capable of being resynthesized to give novel antibacterial peptides. An antibacterial composition is characterized by containing one of the novel antibacterial peptides, as active ingredient.
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公开(公告)号：KR1020160135011A
公开(公告)日：2016-11-24
申请号：KR1020150067557
申请日：2015-05-14
Applicant: 한국과학기술원 , 재단법인 지능형 바이오 시스템 설계 및 합성 연구단
CPC classification number: C12P7/24 , C12N9/00 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/63 , C12N15/70 , C12Y102/01044 , C12Y403/01024 , C12Y602/01012
Abstract: 본발명은재조합미생물을이용한신남알데하이드의제조방법에관한것이다.
Abstract translation: 本发明涉及使用重组微生物制备肉桂醛的方法。
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公开(公告)号：KR1020160131825A
公开(公告)日：2016-11-16
申请号：KR1020150105764
申请日：2015-07-27
Applicant: 한양대학교 산학협력단 , 한국과학기술원
Abstract: 본발명의일실시예에따른다공성다중구조체는 3차원으로서로연결된제 1 공극을다수구비한제 1 다공성구조체; 및상기제 1 공극과다른직경으로 3차원으로서로연결된제 2 공극을다수구비하고, 상기제 1 다공성구조체를둘러싸접합된제 2 다공성구조체;를포함한다. 또한, 본발명의다른실시예에따른다공성다중구조체는마이크로직경을갖고 3차원으로서로연결된다수의제 1 공극및 상기제 1 공극주위에상기제 1 공극보다작은직경으로 3차원으로서로연결된제 2 공극을다수구비하는프레임을포함한다.
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公开(公告)号：KR101582114B1
公开(公告)日：2016-01-21
申请号：KR1020150063963
申请日：2015-05-07
Applicant: 한양대학교 산학협력단 , 한국과학기술원
CPC classification number: Y02P70/56
Abstract: 본발명의일실시예에따른다공성구조체는다수의연결통로를통해 3차원으로서로연결된공극을다수구비한프레임으로구성된다. 본발명의일실시예에따른다공성구조체는프레임에의해구현된다수의공극이최조밀분포상태를갖고, 대칭구조로형성된다수의연결통로에의해다수의공극이서로 3차원으로연결되므로공극률을극대화할수 있는효과가있다.
Abstract translation: 本发明涉及具有改善孔隙率的多孔结构及其制造方法。 根据本发明的一个实施例的多孔结构具有通过多个连接路径三维互连的多个孔的框架。 根据本发明的一个实施例的多孔结构具有能够最大化孔隙率的效果，因为由框架实现的孔具有最接近的分布状态，并且通过对称形成的连接路径三维地互连孔。
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Patent Agency Ranking
公开(公告)号：KR1020150132813A
公开(公告)日：2015-11-26
申请号：KR1020150155169
申请日：2015-11-05
Applicant: 한국과학기술원 , 재단법인 지능형 바이오 시스템 설계 및 합성 연구단
CPC classification number: C07K7/06 , C07K7/08 , C07K14/001 , C07K14/463 , C07K14/47 , C07K14/4723 , C07K2319/00 , C07K2319/21 , C12N9/20 , C12P7/649 , C12Y301/01003
Abstract: 본발명은리파아제활성이증대된양친매성펩타이드(amphipathic peptide) 및리파아제가연결된양친매성펩타이드-리파아제결합체, 상기결합체를코딩하는폴리뉴클레오티드, 상기폴리뉴클레오티드를포함하는발현벡터, 상기발현벡터가도입된형질전환체, 상기결합체의제조방법, 상기결합체를이용한지질의분해방법및 상기리파아제를이용한바이오디젤의생산방법에관한것이다.
Abstract translation: 本发明涉及具有改善的脂肪酶活性的两亲肽，与脂肪酶连接的两亲性肽 - 脂肪酶偶联物，编码缀合物的多核苷酸，包含多核苷酸的表达载体，引入表达载体的转化体， 缀合物，使用该缀合物的脂质分解方法，以及使用该脂肪酶的生物柴油的制备方法。
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公开(公告)号：KR1020140112612A
公开(公告)日：2014-09-24
申请号：KR1020130025814
申请日：2013-03-11
Applicant: 대한민국(농촌진흥청장) , 한국과학기술원
IPC: A61K36/21 , A61K36/258 , A61K31/70 , A61P17/00
CPC classification number: A61K36/258 , A61K8/04 , A61K8/63 , A61K8/97 , A61K31/704 , A61K36/185 , A61K2236/33 , A61K2300/00
Abstract: The present invention relates to a cosmetic composition for alleviating wrinkles and, more specifically, to a cosmetic composition comprising natural herbal extracts. Further, by comprising the herbal extract as a cosmetic base material, the present invention has effects of raising the skin moisture by promoting the synthesis of hyaluronic acid and alleviating wrinkles by inhibiting the synthesis of catabolic enzymes of skin tissues.
Abstract translation: 本发明涉及一种用于缓解皱纹的化妆品组合物，更具体地说，涉及包含天然草药提取物的化妆品组合物。 此外，通过将草药提取物作为化妆品基材，通过促进透明质酸的合成，抑制皮肤组织的分解代谢酶的合成来缓和皱纹，具有提高皮肤水分的效果。
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公开(公告)号：KR101433661B1
公开(公告)日：2014-08-26
申请号：KR1020120058838
申请日：2012-05-31
Applicant: 한국과학기술원 , 재단법인 지능형 바이오 시스템 설계 및 합성 연구단
Abstract: 본 발명은 신규한 진세노사이드 글리코시다제(Ginsenoside glycosidase) 단백질, 상기 단백질을 코딩하는 핵산을 포함하는 벡터가 도입된 형질전환체, 또는 상기 형질전환체의 배양물을 이용하여 PPD(protopanaxadiol) 타입의 사포닌을 전환시켜서 진세노사이드 F2를 제조하는 방법에 관한 것으로, 구체적으로 패니바실러스 무실라지노서스(
paenibacillus
mucilaginosus ) 유래의 단백질을 이용한 것으로, 상기 단백질, 형질전환체 또는 이의 배양물을 유효성분으로 포함하는, PPD 타입의 사포닌의 체내 흡수가 용이한 형태의 가용성 사포닌인 진세노사이드 F2로의 전환용 조성물, 신규한 진세노사이드 글리코시다제 단백질, 상기 단백질을 코딩하는 핵산, 상기 핵산을 포함하는 벡터 및 상기 벡터가 도입된 형질 전환체에 관한 것이다.-
公开(公告)号：KR1020140006683A
公开(公告)日：2014-01-16
申请号：KR1020120074221
申请日：2012-07-06
Applicant: 한국과학기술원 , 재단법인 지능형 바이오 시스템 설계 및 합성 연구단
Abstract: The present invention relates to a method for manufacturing an available minor saponin by converting a PPD-type or PPT-type saponin using a novel ginsenoside glycosidase protein, a transformant in which a vector including a nucleic acid encoding the protein is introduced, a cultured product of the transformant and, more particularly, using a novel ginsenoside glycosidase protein derived from Flavobacterium johnsoniae; a composition, containing the protein, the transformant, or the cultured product of the transformant as active ingredients, for being converted into an available saponin in a form of easily absorbing a PPD-type or PPT-type saponin; a novel ginsenoside glycosidase protein; a nucleic acid encoding the protein; a vector including the nucleic acid; and a transformant in which the vector is introduced. [Reference numerals] (A) PPD type ginsenosides (Substrate); (AA,CC,EE) Voltage [mV]; (B) After α-L-arabinofuranosidase treatment; (BB,DD,FF) Time [minute]; (C) After Bgl B � 10 treatment
Abstract translation: 本发明涉及使用新型人参皂苷糖苷酶蛋白转化PPD型或PPT型皂苷的方法，其中引入了包含编码该蛋白的核酸的载体的转化体，培养产物 更具体地，使用衍生自约氏黄杆菌的新型人参皂苷糖苷酶蛋白; 一种组合物，其含有作为活性成分的转化体的蛋白质，转化体或培养产物，以易于吸收PPD型或PPT型皂苷的形式转化成可用的皂角苷; 人参皂甙苷新蛋白; 编码该蛋白质的核酸; 包含核酸的载体; 和其中引入载体的转化体。 （A）PPD型人参皂苷（底物）; （AA，CC，EE）电压[mV]; （B）α-L-阿拉伯呋喃糖苷酶处理后; （BB，DD，FF）时间[分钟]; （C）Bgl B治疗后10次
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