公开(公告)号：KR1020030077363A

公开(公告)日：2003-10-01

申请号：KR1020020016445

申请日：2002-03-26

Applicant: 한국과학기술원

Inventor： 김선창 ,  박인엽 ,  조주현

IPC: C07K7/08

CPC classification number: C07K14/43527 ,  A61K38/00 ,  C07K7/08 ,  C07K14/001 ,  C07K14/4723 ,  Y02A50/481

Abstract: PURPOSE: Provided are antimicrobial peptide derivatives which involve powerful antimicrobial activity with respect to gram positive bacillus, gram negative bacillus and fungus. They powerfully inhibit microbial expression at the wound portion while not exhibiting any hemolysis, and are used as antimicrobial agent for the wound treatment facilitation, trauma treatment, mouthwash, and eyewash. CONSTITUTION: The antimicrobial peptide comprises an amino acid sequence expressed by formula 1(N-terminal-X1X2X3X4X5X6X7X8X9X10X11X12X13X14X15-C-terminal), wherein X1 is vacant or a basic amino acid, X2 is two hydrophobic amino acids identical with or different from each other, and X3 is a basic amino acid. Furthermore, in the formula 1, X4 is glutamine or asparagine, X5 is phenylalanine or tryptophan, X6 is proline, X7 is isoleucine or valine, X8 is glycine, X9 is a basic amino acid, X10, X12 and X14 are two hydrophobic amino acids identical with or different from each other, respectively, X11 and X13 are basic amino acids identical with or different from each other, respectively, and X15 is vacant or a basic amino acid.

Abstract translation: 目的：提供抗菌肽衍生物，其涉及革兰氏阳性杆菌，革兰氏阴性杆菌和真菌具有强大的抗微生物活性。 它们强力地抑制伤口部分的微生物表达，而不显示任何溶血作用，并且用作伤口治疗促进，创伤治疗，漱口水和洗眼剂的抗微生物剂。 构成：抗微生物肽包含由式1表示的氨基酸序列（N-末端-X 1 X 2 X 3 X 4 X 5 X 6 X 7 X 8 X 9 X 10 X 11 X 12 X 13 X 14 X 15 C-末端），其中X 1为空位或碱性氨基酸，X 2为彼此相同或不同的2个疏水性氨基酸， X3是碱性氨基酸。 此外，在式1中，X4为谷氨酰胺或天冬酰胺，X5为苯丙氨酸或色氨酸，X6为脯氨酸，X7为异亮氨酸或缬氨酸，X8为甘氨酸，X9为碱性氨基酸，X10为X12，X14为两个疏水性氨基酸 分别相同或不同，X 11和X 13分别是彼此相同或不同的碱性氨基酸，X15为空位或碱性氨基酸。

公开(公告)号：KR1020020051124A

公开(公告)日：2002-06-28

申请号：KR1020000080607

申请日：2000-12-22

Applicant: 한국과학기술원 ,  이한웅 ,  주식회사 바이오비젠

Inventor： 이한웅 ,  박찬배 ,  김선창 ,  박인엽

IPC: C07K14/46

CPC classification number: C07K14/463 ,  A61K38/1703

Abstract: PURPOSE: Provided is an anti-cancer agent having Buforin and their derivatives as its active ingredient. The Buforin derivative, BUF(5-13)(RLLR)3 has amino acid sequence of RAGLQFPVGRLLRRLLRRLLR and selectively acts on cancer cells in vivo and vitro. CONSTITUTION: Buforin is characterized by as follows: having broader antibacterial spectrum than conventional antibiotics; showing selective antibacterial effect on not a host cell but a pathogen; and being mass-produced by genetic manipulation by prokaryotic microorganism. The anti-cancer agent includes the Buforin derivative, BUF(5-13)(RLLR) as an active ingredient, and a pharmaceutically acceptable carrier.

Abstract translation: 目的：提供具有Buforin及其衍生物作为其活性成分的抗癌剂。 Buforin衍生物BUF（5-13）（RLLR）3具有RAGLQFPVGRLLRRLLRRLLR的氨基酸序列，并在体内和体外选择性地作用于癌细胞。 构成：Buforin的特征如下：具有比常规抗生素更广泛的抗菌谱; 对宿主细胞但病原体显示出选择性的抗菌作用; 并通过原核微生物的遗传操作大规模生产。 抗癌剂包括Buforin衍生物，作为活性成分的BUF（5-13）（RLLR）和药学上可接受的载体。

公开(公告)号：KR100441402B1

公开(公告)日：2004-07-23

申请号：KR1020020016445

申请日：2002-03-26

Applicant: 한국과학기술원

Inventor： 김선창 ,  박인엽 ,  조주현

IPC: C07K7/08

CPC classification number: C07K14/43527 ,  A61K38/00 ,  C07K7/08 ,  C07K14/001 ,  C07K14/4723 ,  Y02A50/481

Abstract: Disclosed is a novel antimicrobial peptide having excellent antimicrobial activities, its analogs and an antimicrobial composition comprising them. The antimicrobial peptide alternatively comprises basic amino acid residues and hydrophobic amino acid residues, and is able to penetrate into microbial cells and act against a wide variety of microorganisms.

Abstract translation: 公开了具有优异抗微生物活性的新型抗微生物肽，其类似物和包含它们的抗微生物组合物。 抗微生物肽可选地包含碱性氨基酸残基和疏水性氨基酸残基，并且能够穿透微生物细胞并对抗各种各样的微生物。

公开(公告)号：KR100330136B1

公开(公告)日：2002-03-27

申请号：KR1019990005078

申请日：1999-02-12

Applicant: 한국과학기술원

Inventor： 김선창 ,  박인엽 ,  박찬배

IPC: A61K38/10

Abstract: PURPOSE: Provided are novel antibacterial peptide parasin I separated from parasilurus asotus, showing strong antibacterial activity and the use thereof for treatment of wound, cleansing of the oral cavity. CONSTITUTION: A novel antibacterial peptide has the amino acid sequence of KGRGKGGKVRAKAKTRSS (I). The amino acid sequence is capable of being resynthesized to give novel antibacterial peptides. An antibacterial composition is characterized by containing one of the novel antibacterial peptides, as active ingredient.

公开(公告)号：KR100836596B1

公开(公告)日：2008-06-10

申请号：KR1020070006277

申请日：2007-01-19

Applicant: 한국과학기술원

Inventor： 김선창 ,  박인엽 ,  김정민

IPC: C07K14/00 ,  A61K38/10

Abstract: A novel antibacterial peptide is provided to show strong antibacterial activity on a wider range of microorganism species compared with a conventional antibacterial peptide and have less or no toxicity than the conventional antibacterial peptide. A peptide having the antibacterial activity in a highly concentrated salt is represented by a formula(1) of N-terminal-A P K A M X^1 X^2 X^3 X^4 X^5 L Q K K G I-C-terminal, wherein A is alanine; P is proline; K is lysine; M is methionine; X^1 is one basic amino acid; X^2 is two hydrophobic amino acids selected from the group consisting of alanine, valine and leucine; X^3 is two basic amino acids; X^4 is two hydrophobic amino acids selected from the group consisting of alanine, valine and leucine; X^5 is one basic amino acid; L is leucine; Q is glutamine; G is glycine; and I is iso-leucine. An antibacterial composition comprises the peptide as an effective ingredient.

Abstract translation: 提供了一种新型抗菌肽，与常规抗菌肽相比，在更广泛的微生物种类上显示出强的抗菌活性，并且具有比常规抗菌肽更少或没有毒性。 在高浓度盐中具有抗菌活性的肽由N末端-A P K A M X 1 X X 2 X 3 3 X 4 X 5 L Q K K G I-C-末端的式（1）表示，其中A是丙氨酸; P是脯氨酸; K为赖氨酸; M是甲硫氨酸; X ^ 1是一种碱性氨基酸; X ^ 2是选自丙氨酸，缬氨酸和亮氨酸的两种疏水性氨基酸; X ^ 3是两种碱性氨基酸; X ^ 4是选自丙氨酸，缬氨酸和亮氨酸的两种疏水性氨基酸; X ^ 5是一种碱性氨基酸; L是亮氨酸; Q是谷氨酰胺; G是甘氨酸; 我是异亮氨酸。 抗菌组合物包含该肽作为有效成分。

公开(公告)号：KR1019990077430A

公开(公告)日：1999-10-25

申请号：KR1019990005078

申请日：1999-02-12

Applicant: 한국과학기술원

Inventor： 김선창 ,  박인엽 ,  박찬배

IPC: A61K38/10

Abstract: 본발명은어류의일종인메기(Parasilurus asotus)의손상된점막층으로부터분리한신규한항균펩타이드와그 유도체및 이들의용도에관한것으로어류의일종인메기의표피에임의로상처를내고상처입은상피점막층으로부터순수한항균펩타이드파라신 I을분리, 정제한후 분자량및 아미노산염기서열을결정하고상기항균펩타이드파라신 I의유도체를제조한다음항균펩타이드파라신 I의구조를분석하고항균펩타이드파라신 I과그 유도체의항균활성과용혈활성을측정한결과, 항균펩타이드파라신 I은분자량이약 2,000.4Da인 19개의아미노산잔기를전체또는일부로함유하고있으며그람양성균, 그람음성균및 진균등에대하여강력한항균작용을나타내상처부위에서미생물의발현을강력하게억제하고용혈활성은거의나타내지않으므로상처치료촉진제, 외상치료제, 구강청정제및 안약등의항균제로서이용될수 있는뛰어난효과가있다.