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51.
公开(公告)号:AR047152A1
公开(公告)日:2006-01-11
申请号:ARP040104737
申请日:2004-12-17
Applicant: BASF AG
Inventor: KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
IPC: C12N9/02 , C12N15/77 , C12N15/31 , C12N15/67 , C12N15/63 , C12P13/08 , C12P13/12 , C12R1/15 , C12N1/21 , C12R1/13
Abstract: Se refiere al uso de secuencias de ácidos nucleicos para la regulacion de la transcripcion y la expresion de genes, a los nuevos promotores y unidades de expresion propiamente dichos, a procedimientos para la modificacion o activacion del grado de transcripcion y/o grado de expresion de genes, a casetes de expresion que contienen las unidades de expresion, a microorganismos modificados genéticamente con grado de transcripcion y/o grado de expresion modificado o activado, así como también a procedimientos para la preparacion de productos biosintéticos por medio del cultivo de los microorganismos genéticamente modificados. Reivindicacion 1: Uso de un ácido nucleico con actividad promotora, que contiene: A) la secuencia de ácidos nucleicos SEQ. ID. NO. 1 o B) una secuencia derivada de esta secuencia por sustitucion, insercion o delecion de nucleotidos, la que presenta una identidad de por lo menos 90 % a nivel de ácidos nucleicos con la secuencia SEQ. ID. NO. 1, o C) una secuencia de ácidos nucleicos, que hibrida con la secuencia de ácidos nucleicos SEQ. ID. NO. 1 bajo condiciones severas o D) fragmentos funcionalmente equivalentes de las secuencias de A), B) o C), para la transcripcion de genes.
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公开(公告)号:AR047058A1
公开(公告)日:2006-01-04
申请号:ARP040104742
申请日:2004-12-17
Applicant: BASF AG
Inventor: ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN , KROEGER BURKHARD , KIEFER PATRICK , HEINZLE ELMAR , WITTMANN CHRISTOPH
IPC: C12P20060101 , C12P13/08 , C12N15/54 , C12N1/21 , C12R1/15
Abstract: Comprende métodos para aumentar la produccion de un compuesto químico fino, por ejemplo, lisina a partir de un microorganismo, por ejemplo, Corynebacterium mediante la desregulacion de un gen codificador de una enzima, es decir, glicerol quinasa. Se proveen métodos para incrementar la produccion de lisina en Corynebacterium glutamicum a través del incremento de la expresion de la actividad de glicerol quinasa. También se provee un proceso para la produccion de lisina a través de la regulacion del flujo de carbono oxalacetato (OAA).Se proveen métodos para la produccion de lisina a través de la utilizacion de fructosa o sacarosa como fuente de carbono. Reivindicacion 1: Un método para aumentar el flujo metabolico a través de la vía de pentosafosfatos en un microorganismo que comprenda el cultivo de dicho microorganismo que contenga un gen que se desregula bajo condiciones tales que se aumenta el flujo metabolico a través de la vía de pentosafosfatos. Reivindicacion 49: Un microorganismo recombinante que comprende un gen de biosíntesis de pentosafosfato desregulado.
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公开(公告)号:DE10359595A1
公开(公告)日:2005-07-28
申请号:DE10359595
申请日:2003-12-18
Applicant: BASF AG
Inventor: KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
Abstract: Use of a nucleic acid (I) with promoter activity for transcribing genes, where (I) is (a) a 164 bp sequence (SEQ ID No,:1); (b) a variant of (SEQ ID No.:1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; (c) a sequence that hybridizes to (SEQ ID No.:1) under stringent conditions; or (d) a functionally equivalent fragment of (a)-(c), is new. Independent claims are also included for : (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence ggaggga (53) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequence tagagt (52) as a -10 region for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (52) or (53).
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公开(公告)号:DE10359594A1
公开(公告)日:2005-07-28
申请号:DE10359594
申请日:2003-12-18
Applicant: BASF AG
Inventor: KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
IPC: C07K14/34 , C12N20060101 , C12N1/00 , C12N15/63 , C12N15/67 , C12N15/77 , C12P1/00 , C12P13/08 , C12P13/12
Abstract: Use, for transcribing genes, of a nucleic acid (I) with promoter activity, where (I) is a 186 base pair sequence (1), reproduced; a variant of (1) with at least 90% identity and derived by substitution, insertion or deletion of nucleotides; a sequence that hybridizes to (1) under stringent conditions; or a functionally equivalent fragment of them, is new. Independent claims are also included for the following: (1) use of an expression unit (EU), containing (I) and functionally linked to a sequence (X) that ensures translation of RNA, for expressing genes; (2) (I), other than sequence (1), i.e. (Ia), as new compounds; (3) EU that contain (Ia) linked to (X); (4) altering (or producing) the transcription rate of genes in a microorganism, relative to the wild type; (5) expression cassette (EC) comprising at least one EU of (1), at least one other nucleic acid sequence (to be expressed) and optionally additional gene control elements, where at least the first two are linked and the sequence being expressed is heterologous with respect to EU; (6) expression vector (EV) that contains EC; (7) genetically modified microorganisms (GMO) having, for at least one gene, an altered (or induced) transcription rate, relative to the wild type; (8) preparing biosynthetic products by culturing GMO of (7); (9) use of the sequence aggagga (42) as a ribosome-binding site in expression units that provide expression of genes in Corynebacterium or Brevibacterium; (10) use of the sequences tagttt (39), taggat (40) or tgcgct (41) as -10 regions for expression of genes in Corynebacterium or Brevibacterium; and (11) expression units that contain sequences (39)-(42).
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公开(公告)号:CA2547792A1
公开(公告)日:2005-06-30
申请号:CA2547792
申请日:2004-12-16
Applicant: BASF AG
Inventor: HAEFNER STEFAN , KROEGER BURKHARD , ZELDER OSKAR , SCHROEDER HARTWIG , HABERHAUER GREGOR , RUFFER UWE , KLOPPROGGE CORINNA , GRAEF CLAUDIA ISABELLA
Abstract: The invention relates to mutant nucleic acids and proteins from the metaboli c pathway of fine chemicals, methods for the production of genetically modifie d production organisms, methods for the production of fine chemicals by cultivating said genetically modified organisms, and said genetically modifi ed organisms.
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公开(公告)号:TR200500004T2
公开(公告)日:2005-03-21
申请号:TR200500004
申请日:2000-06-23
Applicant: BASF AG
Inventor: POMPEJUS MARKUS , KROEGER BURKHARD , SCHROEDER HARTWIG , ZELDER OSKAR , HABERHAUER GREGOR
IPC: G01N33/53 , C07K20060101 , C07K14/34 , C12N20060101 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/00 , C12N9/12 , C12N9/18 , C12N9/88 , C12N15/09 , C12N15/31 , C12N15/55 , C12P20060101 , C12P13/04 , C12P13/08 , C12P21/02 , C12Q20060101 , C12Q1/68 , C12R1/15 , G01N33/566 , G01N33/569
Abstract: Isolated nucleic acid molecules, designated SMP nucleic acid molecules, which encode novel SMP proteins from Corynebacterium glutamicum are described. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing SMP nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides isolated SMP proteins, mutated SMP proteins, fusion proteins, antigenic peptides and methods for the improvement of production of a desired compound from C. glutamicum based on genetic engineering of SMP genes in this organism.
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公开(公告)号:BR0213771A
公开(公告)日:2004-10-19
申请号:BR0213771
申请日:2002-10-31
Applicant: BASF AG
Inventor: ZELDER OSKAR , POMPEJUS MARKUS , SCHROEDER HARTWIG , KROEGER BURKHARD , KLOPPROGGE CORINNA , HABERHAUER GREGOR
Abstract: Isolated nucleic acid (I) that encodes any of 50 amino acid sequences (II) that have, at specific positions, an amino acid (aa) alteration. The specification includes (i) a table identifying the function of (II) and the nature/position of the amino acid exchange and (ii) sequences for (I) and (II). Independent claims are also included for the following: (1) vector that contains at least one (I); (2) host cell transfected with at least one vector of component (1); and (3) preparing fine chemicals by culturing cells of component (2).
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公开(公告)号:DE10239308A1
公开(公告)日:2004-03-11
申请号:DE10239308
申请日:2002-08-27
Applicant: BASF AG
Inventor: KROEGER BURKHARD , ZELDER OSKAR , KLOPPROGGE CORINNA , SCHROEDER HARTWIG , HAEFNER STEFAN
Abstract: Fermentative production of at least one sulfur-containing fine chemical (I) by fermenting a (I)-producing coryneform bacterium (A), concentrating (I) in medium or cells, then isolating it. (A) expresses at least one heterologous nucleotide sequence (II) that encodes a protein (III) with methionine synthase (metF) activity. An Independent claim is also included for preparation of a feed additive (B) that contains L-methionine by culturing/fermenting an L-Met producing microorganisms, removing water from the fermentation broth and optionally also up to 100 % of the biomass, then drying the treated broth to recover (B) in powdered or granular form.
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公开(公告)号:DE10154276A1
公开(公告)日:2003-05-15
申请号:DE10154276
申请日:2001-11-05
Applicant: BASF AG
Inventor: ZELDER OSKAR , POMPEJUS MARKUS , SCHROEDER HARTWIG , KROEGER BURKHARD , KLOPPROGGE CORINNA , HABERHAUER GREGOR
Abstract: The invention relates to novel nucleic acid molecules, the use thereof in the construction of bio-engineered improved microorganisms and to methods for the production of fine chemicals, especially amino acids with the aid of said bio-engineered improved microorganisms.
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公开(公告)号:DE10154270A1
公开(公告)日:2003-05-15
申请号:DE10154270
申请日:2001-11-05
Applicant: BASF AG
Inventor: ZELDER OSKAR , POMPEJUS MARKUS , SCHROEDER HARTWIG , KROEGER BURKHARD , KLOPPROGGE CORINNA , HABERHAUER GREGOR
Abstract: The invention relates to novel nucleic acid molecules, to the use thereof in the construction of genetically improved microorganisms and to a method for producing fine chemical products, in particular amino acids, by means of said genetically improved microorganisms.
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