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    55.
    发明专利
    未知

    公开(公告)号：IT1162487B

    公开(公告)日：1987-04-01

    申请号：IT4946979

    申请日：1979-06-20

    Applicant: MILES LAB

    Inventor： HORNBY WILLIAM E ,  MORRIS DAVID L

    IPC: C12N9/96 ,  G01N33/542 ,  G01N33/78

    Abstract: A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

    FENCLOFENAC AS TBP BLOCKING AGENT IN IODOTHYRONINE IMMUNOASSAYS
    56.
    发明专利
    FENCLOFENAC AS TBP BLOCKING AGENT IN IODOTHYRONINE IMMUNOASSAYS 未知

    公开(公告)号：DE3273222D1

    公开(公告)日：1986-10-16

    申请号：DE3273222

    申请日：1982-10-25

    Applicant: MILES LAB

    Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

    IPC: G01N33/78

    Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

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