公开(公告)号：NO823658L

公开(公告)日：1983-05-05

申请号：NO823658

申请日：1982-11-03

Applicant: MILES LAB

Inventor： ATKINSON DAVID C ,  CARRICO ROBERT JOSEPF ,  MORRIS DAVID L

IPC: G01N33/78 ,  G01N

Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

公开(公告)号：DK488682A

公开(公告)日：1983-05-05

申请号：DK488682

申请日：1982-11-03

Applicant: MILES LAB

Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

IPC: G01N33/78 ,  G01N

Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

公开(公告)号：AT22182T

公开(公告)日：1986-09-15

申请号：AT82109830

申请日：1982-10-25

Applicant: MILES LAB

Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

IPC: G01N33/78

公开(公告)号：CA1208547A

公开(公告)日：1986-07-29

申请号：CA414812

申请日：1982-11-03

Applicant: MILES LAB

Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

IPC: G01N33/78 ,  G01N33/566 ,  G01N33/573

Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or plasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.

公开(公告)号：NO823658A

公开(公告)日：1983-05-05

申请号：NO823658

申请日：1982-11-03

Applicant: MILES LAB

Inventor： ATKINSON DAVID C ,  CARRICO ROBERT JOSEPF ,  MORRIS DAVID L

IPC: G01N33/78 ,  G01N

CPC classification number: G01N33/78 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/826

公开(公告)号：DE3273222D1

公开(公告)日：1986-10-16

申请号：DE3273222

申请日：1982-10-25

Applicant: MILES LAB

Inventor： ATKINSON DAVID C ,  CARRICO ROBERT J ,  MORRIS DAVID L

IPC: G01N33/78

Abstract: An improved immunoassay method, reagent means, and test kit for determining an iodothyronine, e.g., thyroxine (T-4), in a biological fluid, usually serum or piasma, wherein fenclofenac and related phenylacetic acids, or salts thereof, are employed as novel blocking agents for the binding of iodothyronines to thyroxine binding protein (TBP). The present invention is particularly advantageous as applied to homogeneous competitive binding iodothyronine immunoassays wherein a spectrophotometric response is generated in the assay reaction mixture at a wavelength greater than about 300 nm, the blocking agents of the present invention having been found to have no substantial absorbance at wavelengths above 300 nm. Such homogeneous immunoassays include those which employ labels such as fluorescers, enzyme substrates, enzyme prosthetic groups, enzymes, and enzyme inhibitors.